Perkin-Elmer MultiScope System, User Reference, Page: 64 / 142

64 . MultiScope System Microscope User's Reference
Preparing Samples
Using a microscope for infrared spectroscopy can simplify sample preparation,
because you can examine the sample visually and select the best part to use.
Proper sample preparation is still important, however, if you are to collect good
quality spectra.
•
The sample must be thin enough (approximately 5 to 20 µm) to give good
detail and undistorted absorption bands.
• The area of the sample must be large enough to give an adequate signal;
otherwise, the scan time must be increased.
Preparing a sample, therefore, often involves flattening it; this both thins it and
increases its area. Flattening is accomplished by rolling, squeezing, or pressing the
sample.
This chapter tells you how to prepare samples for spectroscopy with the
microscope. It includes:
• A list of useful tools;
•
A list of window materials commonly used for mounting samples;
• Descriptions of special techniques used to prepare particular types of samples.
For hands-on experience in preparing samples of fibers and of coatings on reflective
surfaces, we suggest that you try the tutorial in Tutorial: Using the Microscope .