2
CHAPTER 1
•
INTRODUCTION.......................................................................................................................6
O
VERVIEW
...........................................................................................................................................................6
W
ELCOME
! .........................................................................................................................................................7
L
IST OF
E
QUIPMENT
.............................................................................................................................................7
G
ETTING
S
TARTED
...............................................................................................................................................7
C
HLOROPHYLL
F
LUORESCENCE
...........................................................................................................................8
D
EFINITIONS
: .....................................................................................................................................................
11
Q
UANTUM PHOTOSYNTHETIC YIELD OF
PSII
–
AN IN DEPTH DISCUSSION OF ITS VALUE AND LIMITATIONS
. .......
16
Q
UENCHING
M
EASUREMENTS
,
AN
O
VERVIEW
...................................................................................................
21
Quenching equations: .................................................................................................................................. 24
Definitions - lake model parameters ............................................................................................................ 25
Definitions - puddle model parameters........................................................................................................ 26
Understanding the quenching mode trace ................................................................................................... 28
More helpful hints for setting test variable in quenching protocols. ........................................................... 33
D
ARK
A
DAPTATION
–
H
OW
L
ONG IS
L
ONG
E
NOUGH
? .......................................................................................
35
R
ELATIVE
E
LECTRON
T
RANSPORT
R
ATE
...........................................................................................................
37
B
IBLIOGRAPHY
..................................................................................................................................................
40
CHAPTER 2
•
THE OS1P..................................................................................................................................52
I
NTRODUCTION
..................................................................................................................................................
52
K
EY
F
EATURES
..................................................................................................................................................
53
P
HYSICAL
F
EATURES
P
ANEL
P
HOTOGRAPHS
...................................................................................................
54
H
ARDWARE
O
VERVIEW AND
M
EASUREMENT
P
RINCIPLES
.................................................................................
56
L
IGHT SOURCES
.................................................................................................................................................
57
Modulated light source ................................................................................................................................ 57
Saturation pulse light source ....................................................................................................................... 57
The LED actinic light source ....................................................................................................................... 58
The far-red light source .............................................................................................................................. 58
T
HE
F
IBER
O
PTIC
L
IGHT
G
UIDE
.........................................................................................................................
58
E
LECTRONICS
....................................................................................................................................................
59
H
OUSING AND
C
ARRYING
C
ASE
.........................................................................................................................
59
CHAPTER 3
•
OPERATING THE OS1P......................................................................................................... 60
I
NTRODUCTION
..................................................................................................................................................
60
I
NTRODUCTION TO RUNNING TESTS
....................................................................................................................
60
Set up menu .................................................................................................................................................. 61
Adjust screen touch sensitivity ..................................................................................................................... 62
Touch Panel Calibrate ................................................................................................................................. 62
Test menu ..................................................................................................................................................... 63
F
V
/F
M
P
ROTOCOL
(
OR
F
V
/
M ON THE SCREEN
)..................................................................................................
64
Cookbook check list before making Fv/Fm measurements .......................................................................... 65
Running the Fv/Fm test ................................................................................................................................ 67
Drill down view of Fv/Fm measuring screens ............................................................................................. 68
Changing Fv/Fm measuring parameters - ................................................................................................... 69
Setting the modulation light source intensity ............................................................................................... 69
New -automated modulation light set up ..................................................................................................... 69
Far red light ................................................................................................................................................. 70
Setting the saturation pulse intensity ........................................................................................................... 70
Setting saturation pulse duration ................................................................................................................. 70
Entering a note with a measurement............................................................................................................ 72
