
4.1 Initial start-up
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850 Professional IC–Anion – MSM-HC – MCS – LP Gradient
4 Start-up
The chapter
Start-up
is divided into 2 sections:
Initial start-up
The
initial start-up
is carried out during the
ini-
tial installation
.
Conditioning
Conditioning is carried out as a final installation
step and each time after the system is started.
4.1
Initial start-up
The initial start-up is carried out during the initial installation. The entire
system is flushed before guard column and separation column are
installed.
CAUTION
The separation column and guard column may not be installed for the
initial start-up.
Make sure that the 6.2744.040 coupling is being used instead of the
columns.
Perform the following steps during the initial start-up:
1 Preparing the software
■
Start the PC program
MagIC Net
.
■
Open the
Equilibration
tab in
MagIC Net
.
■
Select (or create) a suitable method.
2 Preparing the instrument
■
Ensure that the eluent aspiration tubing is immersed in the eluent
and that there is enough eluent in the eluent bottle.
■
Switch on the instrument.
3 Deaerating the high pressure pump
■
Deaerate the high pressure pump(s) via the purge valve