Malvern Zetasizer Nano Series, Accessories Manual

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Advanced protein features Chapter 6
Zetasizer Nano accessories guide Page 6-15
The parameters are described below.
Data
This indicates the data entries that have been entered into the Debye plot table, see
below.
Sample

Sample dn/dc (ml/g)
This is the specific refractive index increment; the change in refractive index as
a function of the change in concentration.

Experiment notes
Used to record specific details about the experiment or calculation performed.

Hydrodynamic radius (nm)
The radius as measured using dynamic light scattering.

Solvent Ref. Index (Refractive index)
The refractive index of the solvent used.

Solvent count rate (kcounts)
The count rate used to calculate the Rayleigh ratio (R

) is the ‘residual’ count
rate, which is derived by subtracting the solvent count rate from the sample
count rate.

Shape Model
The shape model that is used to estimate the radius of gyration from the
hydrodynamic radius, and therefore calculate the angle dependent effects on
KC/RoP for particles of sizes outside the Rayleigh region (Diameter >
~50nm).
System

Wavelength (nm)
The wavelength of the laser used in the Zetasizer Nano instruments, or for the
measurement. Either 632.8nm ‘red’ or 532nm ‘green’ laser wavelengths are
available.

Scattering angle (degrees)
The scattering angle of the optics unit - either 173°, or 90° for the ‘classical’
optics arrangement.

Toluene count rate (kcounts)
The scattering count rate of the toluene reference.
Saving the Debye plot
The parameters and data inputted to produce the plot can be saved by pressing the
Save button, and then reviewed at a later stage by pressing the Load button.