Malvern Zetasizer Nano Series, Accessories Manual

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Advanced protein features Chapter 6
Zetasizer Nano accessories guide Page 6-11
second calculation calculates protein charge from the electrophoretic mobility
and Stokes radius.

Virial Diameter
A tool to calculate the virial diameter, also called the thermodynamic diameter,
from the measured molecular weight and 2
nd
virial coefficient (A2).

DLS Debye plot
A 'dynamic Debye plot' of measured hydrodynamic size as a function of sample
concentration to calculate the true hydrodynamic radius and the dynamic virial
coefficient.

Interparticle distance
A tool to calculate the distance between the particles based on their
concentration and molecular weight. Also estimates the thickness of the
electrostatic layer based on protein charge and ionic strength.

Mixture Viscosity
A simple tool that calculates the overall viscosity of a mixture of solutions based
on a volume weighted mean viscosity.

Oligomer ratios
A tool that estimates the ratio between monomer and dimer in a peak that
contains both where the size of each is known. This is based on work published
by Malvern Instruments entitled "Dynamic light scattering as a relative tool for
assessing the molecular integrity and stability of monoclonal antibodies" by
Nobbmann U et al. The reference is published in Biotechnology and Genetic
engineering Reviews, 2007, Vol 24 pp117-128.
In each case the effect of changing any of the input parameters can be seen instantly
in all of the derived parameters.