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Chapter 4 – Fluorescence Cell Counting
4.1 Sample Preparation
4.1.1 Prepare the following materials to count cells
Cell sample
Luna™ cell counting slides (cat# L12001, L12002, L12003 or L12004)
AO/PI cell viability kit (cat# F23001)
4.1.2 If desired, insert a USB drive to save data and results,
4.1.3 Transfer 2
l of the AO/PI staining solution to a new 1.5 ml microfuge tube.
4.1.4. Add 18
l of the cell sample to the tube and mix by pipetting up and down or flicking the
bottom of the tube while holding with the other hand.
4.2 Loading Samples into Slides
4.2.1 Holding the edge of the slide, load 10
l of the mixed cell sample into the inlet of one chamber
of the counting slide. For easy and accurate loading, tilt the pipette around 45~60 degrees as
shown below.
<Sample Loading>
