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Without trypan blue
When samples do not contain trypan blue dye, turn on this button and
follow the directions in the message boxes. These instructions set the
“Dilution Factor”. For samples with trypan blue dye, the dilution factor must
be set to value “2”. For samples without trypan blue dye, the dilution factor
must be set to value “1”.
<Dilution Factor Check>
Custom staining
In this option, cell samples can be stained with other dyes (or even the
culture media) for counting. Before choosing this option, the instrument
must be re-calibrated with users’ own stains or culture media. For re-
calibration, please refer to Section 7.3 Calibrating the Counter. When
using this option, make sure that the dilution is 1:1 (cell sample vs.
staining solution) and the “Dilution Factor” must be set to value 2.
