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Kurabo QuickGene-810, Operation Manual

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Kurabo QuickGene-810 Operation Manual Download Page 9

QuickGene-810 Operation Manual 

Rev. 1.7

vii

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Einführung

Diese Anleitung richtet sich an Wissenschaftler.
Wir danken Ihnen für den Kauf des Nukleinsäureisolierungssystems QuickGene-810 (im Folgenden als 
„QuickGene-810“ bezeichnet). Mit diesem Gerät kann durch einfache Bedienung eine DNA- und RNA-
Extraktion durchgeführt werden, unter anderem aus Vollblut-, Gewebe-, Zell-, Pflanzen-, Virus- und 
anderen Proben.
Diese Anleitung enthält wichtige Informationen zur sicheren Verwendung von QuickGene-810. Lesen Sie 
die Anleitung vor der Verwendung sorgfältig durch. 
Bewahren Sie diese Anleitung nach dem Lesen zum späteren Nachschlagen auf.

■ 

Ausschlussklauseln

• Der Inhalt dieser Anleitung kann jederzeit ohne Vorankündigung geändert werden.

• FUJIFILM Corporation übernimmt keine Haftung für die Verletzung von Copyright- oder anderen 

Rechten Dritter, die aus der Verwendung jeglicher Informationen dieser Anleitung entstehen können.

• FUJIFILM Corporation übernimmt keine Haftung für Geräte-Ausfälle oder -Beschädigungen, 

die durch Installation, Standortveränderung, Veränderungen, Wartung oder Instandsetzung 

entstehen, sofern diese nicht durch FUJIFILM Corporation oder von FUJIFILM Corporation 

genannte Auftragnehmer durchgeführt wurden.

• FUJIFILM Corporation übernimmt keine Haftung für Ausfälle und Beschädigungen unserer 

Produkte, die durch Produkte von Drittherstellern verursacht wurden, sofern diese Produkte 

nicht von FUJIFILM Corporation geliefert wurden.

• FUJIFILM Corporation übernimmt keine Haftung für Geräte-Ausfälle und -Beschädigungen, 

die durch Veränderungen, Wartung, Instandsetzung usw. unter Verwendung von Ersatzteilen 

verursacht wurden, die nicht von FUJIFILM Corporation angegebene Originalteile sind.

• FUJIFILM Corporation übernimmt keine Haftung für Geräte-Ausfälle und -Beschädigungen, 

die auf die Nichtbeachtung der in dieser Anleitung beschriebenen Vorsichtsmaßregeln und 

Betriebsverfahren zurückzuführen sind.

• FUJIFILM Corporation übernimmt keine Haftung für Geräte-Ausfälle und -Beschädigungen, die auf 

den Betrieb des Geräts unter Bedingungen zurückzuführen ist, die den in dieser Anleitung 

beschriebenen Betriebsanforderungen (Stromversorgung, Betriebsumgebung etc.) nicht entsprechen.

• FUJIFILM Corporation übernimmt keine Haftung für Geräte-Ausfälle und -Beschädigungen, 

die auf Naturkatastrophen wie Feuer, Erdbeben, Überschwemmung oder Blitzschlag 

zurückzuführen sind.

• Kein Teil dieser Anleitung darf ohne Erlaubnis der FUJIFILM Corporation reproduziert werden.

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Warenzeichen

Firmen- und Produktnamen sind Warenzeichen oder eingetragene Warenzeichen der jeweiligen Inhaber. 

Alle Rechte vorbehalten, Copyright © 2005-2010 FUJIFILM Corporation

Umgang mit infektiösen Stoffen

Befolgen Sie bei der Handhabung oder Entsorgung von infektiösen Abfällen die 
Laborrichtlinien sowie die geltenden Gesetze und Vorschriften, und führen Sie die 
Verbrennung, Einschmelzung, Sterilisation und/oder Desinfektion entsprechend durch.
Wenn Sie die Entsorgung durch Dritte ausführen lassen, beauftragen Sie 
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die relevanten Informationen zur Sondermüll-Abfallbehandlung zur Verfügung.

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KURABO INDUSTRIES

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Summary of Contents for QuickGene-810

Page 1: ...Rev 1 7 Original Instructions Operation Manual Rev 2 1 ...

Page 2: ......

Page 3: ...ual may be reproduced without permission from FUJIFILM Corporation Trademarks Company names and product names are trademarks or registered trademarks of their respective owners All Rights Reserved Copyright FUJIFILM Corporation 2005 2010 About Infectious Materials When handling or disposing of infectious materials follow the laboratory guideline or the law regarding infectious wastes to perform th...

Page 4: ... BIOHAZARD indicates exposure to a biological agent or condition that may be infectious or harmful PINCHING OF FINGERS indicates the possibility of danger that may lead to death or serious injury CAUTION CAUTION indicates the possibility of danger that may lead to minor or moderate personal injury CAUTION may also indicate an accident of property damage Important Important shows the important note...

Page 5: ...iii 1 3 2 5 4 6 7 2005 2010 这是一本用于调查研究的指南手册 INDUSTRIES KURABO KURABO INDUSTRIES KURABO INDUSTRIES KURABO INDUSTRIES KURABO INDUSTRIES KURABO INDUSTRIES KURABO INDUSTRIES KURABO INDUSTRIES KURABO INDUSTRIES INDUSTRIES KURABO KURABO INDUSTRIES ...

Page 6: ...1 2 6 3 4 5 7 iv QuickGene 810 Operation Manual Rev 1 7 ...

Page 7: ...èces d origine spécifiées par FUJIFILM Corporation FUJIFILM Corporation ne peut être tenu pour responsable des défaillances ou des dommages de l appareil dus à la non observation des procédures de fonctionnement et des précautions décrites dans ce manuel FUJIFILM Corporation ne peut être tenu pour responsable des défaillances ou des dommages de l appareil en cas où il est utilisé dans un environne...

Page 8: ...D indique l exposition à un agent biologique ou à une situation qui peut être infectieuse ou nocive PINCHING OF FINGERS signale un danger possible qui s il n était pas évité pourrait entraîner la mort ou des blessures graves CAUTION CAUTION signale un danger possible qui s il n était pas évité pourrait entraîner des blessures corporelles mineures ou légères CAUTION pourrait aussi indiquer un risqu...

Page 9: ...endung von Ersatzteilen verursacht wurden die nicht von FUJIFILM Corporation angegebene Originalteile sind FUJIFILM Corporation übernimmt keine Haftung für Geräte Ausfälle und Beschädigungen die auf die Nichtbeachtung der in dieser Anleitung beschriebenen Vorsichtsmaßregeln und Betriebsverfahren zurückzuführen sind FUJIFILM Corporation übernimmt keine Haftung für Geräte Ausfälle und Beschädigungen...

Page 10: ...t die Gefährdung durch biologische Agenzien oder Verhältnisse die infektiös oder gesundheitsschädlich sein können PINCHING OF FINGERS weist auf die Möglichkeit einer Gefahr hin die zu ernsthaften Verletzungen oder Verletzungen mit Todesfolge führen kann CAUTION CAUTION weist auf gefährliche Betriebsbedingungen hin die zu leichten bis mittleren Verletzungen führen können CAUTION kann darüber hinaus...

Page 11: ...ning liquid on the QuickGene 810 Doing so may cause a device failure fire or electric shock In the event the device overheats starts to smoke or smells strange immediately unplug the power cable Do not insert fingers while the carrier is travelling to the right or left end or the table unit is travelling horizontally CAUTION Never attempt to remodel the QuickGene 810 without the manufacturer s per...

Page 12: ...tc Bringing the QuickGene 810 close to any type of magnetic field may cause a malfunction Product Use Limitations FUJIFILM Corporation assumes no liability for results regardless of the intended use It is the user s responsibility to validate the performance of QuickGene kits and QuickGene 810 for any case of use The QuickGene 810 is a device for research and study only When you use the QuickGene ...

Page 13: ...QuickGene 810 Operation Manual Rev 1 7 xi 1 3 2 5 4 6 7 搬运操作人员行走于右边或左边或者操作台装置水平移动过程中 切勿插入手指 KURABO INDUSTRIES KURABO INDUSTRIES ...

Page 14: ...1 2 6 3 4 5 7 xii QuickGene 810 Operation Manual Rev 1 7 KURABO INDUSTRIES ...

Page 15: ...aisant de provoquer une panne un incendie ou une décharge électrique En cas de surchauffe de l appareil ou s il dégage de la fumée ou une odeur bizarre débranchez immédiatement le câble d alimentation N introduisez pas de doigt en cours de déplacement du chariot vers l extrémité droite ou gauche ou de déplacement horizontal du bloc table CAUTION N essayez en aucun cas de remodeler ou modifier le Q...

Page 16: ... quelconque de champ magnétique il risque d y avoir un dysfonctionnement Limitations d utilisation du produit FUJIFILM Corporation n assume aucune responsabilité de résultats quel que soit l usage voulu Il est de la responsabilité de l utilisateur de valider la performance des kits QuickGene et du QuickGene 810 dans tous les cas d utilisation Le QuickGene 810 est un appareil uniquement destiné à l...

Page 17: ...einem Brand oder Stromschlag kommen Wenn sich das Gerät überhitzt oder wenn Rauch oder ungewöhnliche Gerüche auftreten ziehen Sie sofort das Netzkabel ab Stecken Sie Ihre Finger nicht in das Gerät während der Träger an den Rechts oder Linksanschlag fährt oder die Tischeinheit horizontal verfährt CAUTION Versuchen Sie nicht das QuickGene 810 ohne die Erlaubnis des Herstellers zu verändern Dadurch k...

Page 18: ...dung FUJIFILM Corporation übernimmt keine Haftung für die Ergebnisse unabhängig vom Verwendungszweck Es liegt in der Verantwortung des Anwenders die Eignung der QuickGene Kits und des QuickGene 810 für die jeweils vorgesehene Anwendung zu überprüfen Das QuickGene 810 ist nur für den Einsatz in Forschung und Lehre vorgesehen Wenn Sie das QuickGene 810 einsetzen lesen Sie diese Anleitung sorgfältig ...

Page 19: ...QuickGene 810 Operation Manual Rev 1 7 xvii 1 3 2 5 4 6 7 ...

Page 20: ...1 2 6 3 4 5 7 xviii QuickGene 810 Operation Manual Rev 1 7 ...

Page 21: ...in Specifications 18 3 Operation 3 1 Turn the Power ON and OFF 19 3 2 Preparation 20 3 3 Preparation of Lysate Samples 21 3 4 Extraction Operations 21 3 5 Operations to Restart Program from Pause 28 3 6 Operations to Stop Extraction Process 29 3 7 Quick Guide 29 4 Maintenance 4 1 Before Using the QuickGene 810 31 4 2 When the QuickGene 810 is left for a Week or More 31 4 3 Cleaning the QuickGene 8...

Page 22: ...on Failure 45 6 2 Typical Failure 45 6 3 Error Messages 46 6 4 When the Buffer is Contaminated 46 Appendix A A 1 Usable Power Supply Cords 47 A 2 Spare Parts 48 A 3 About Services 49 A 4 Repair Service Period of Product 49 A 5 Notes for Transfer QuickGene 810 49 A 6 Other Information 49 ...

Page 23: ...cedures This chapter describes how to maintain the QuickGene 810 This chapter explains how to set parameters This chapter describes the procedures that should be taken when you suspect device failures and the messages shown on the LCD This appendix describes after sales services and points to note for transfer 1 Installation 2 Basic Functions 3 Operation 4 Maintenance 5 How to Set Parameters 6 Tro...

Page 24: ...1 2 6 3 4 5 7 xxii QuickGene 810 Operation Manual Rev 1 7 ...

Page 25: ... is flat and stable with no vibration WARNING Do not hold front cover of QuickGene 810 for transportation Do not install the QuickGene 810 to a location where it can be splashed with water It may cause a device failure fire or electric shock When relocating the QuickGene 810 disconnect the plug from the outlet first If the power cable is damaged this may cause a device failure fire injury or elect...

Page 26: ...height Use conditions The following shows the operating environment of the QuickGene 810 Items Specifications Temperature C During operation 15 30 During downtime 0 55 Humidity RH During operation 30 80 During downtime 10 80 Max wet bulb temperature C During operation 29 non condensing During downtime 29 non condensing Temperature gradient C Hr 12 or less non condensing Humidity gradient RH day 30...

Page 27: ...any missing part s or order parts For the spare parts refer to A 2 Spare Parts page 48 QuickGene 810 QuickGene 810 Operation Manual Holder Stand Holder Carriage Cartridge Holder Tube Holder Buffer Stand Adapter Discharge Tray Tube Rack Pump Tube for wash buffer Pump Tube for elution buffer Pump Tube spare tube Tube Cap Large Blue Tube Cap Small Blue Tube Cap Large Purple Collection Tube CT Waste T...

Page 28: ...ront cover and remove the following items Clamps for fixing the carrier both side of the Carrier Remove the tape that holds the tubes Tape Important Keep the shipping box and 2 clamps They will be necessary for transporting QuickGene 810 CAUTION The front cover will not stay on the way to the upper limit If you release the front cover while opening it it will drop suddenly and may cause injury or ...

Page 29: ... 1 Installation 1 3 2 5 4 6 7 1 4 Overview This section explains the name and function of each part in the QuickGene 810 Front and side view Rear view Power Swtich Operation Panel Handle Front Cover Inlet Ventilation Holes Breaker Checkpoint ...

Page 30: ...e conical tube to the Carrier Elution Buffer Pump Sends the elution buffer in the conical tube to the Carrier Carrier Moves automatically during extraction operation Pressure Head Presses out the air and uses the pressure to flush out solutions Wash Buffer Nozzle Provides the wash buffer into Cartridges Elution Buffer Nozzle Provides the elution buffer into Cartridges Sensor Checks that the carrie...

Page 31: ...process is completed The orange light blinks when an error is detected The light is off in other cases 6 ELUTION The green light is on during washing process starting from when the START button is pressed The green light blinks during collection process or when the extraction process is completed The orange light blinks when an error is detected The light is off in other cases Button Note The butt...

Page 32: ...WHOLE BLOOD RNA CELL RNA CELL PLUS DNA TISSUE RNA TISSUE RNA TISSUE PLUS PLASMID Extraction mode names can be changed 12 CR MOVE Moves the carrier from side to side Each time the switch is pressed the carrier moves from side to side Can be used while the front cover is closed 13 Can only be used at setup mode Increments the setting value of the item displayed in the LCD at setup mode Pressing and ...

Page 33: ...conical tube Large Large conical tube for wash buffer BD Falcon 175ml conical tube Small conical tube for elution buffer BD Falcon 50ml conical tube WARNING Ignoring the following notations may lead to fire or electric shock Use a power cable that meets your country s standard or contact your local distributor Si les recommandations suivantes ne sont pas prises en compte un incendie ou une décharg...

Page 34: ... Turn off the power Press the O side of the switch 4 Open the front cover Hold the handle and open the front cover until the upper limit is reached 5 Attach the Pump Tube to the pump Attach the Pump Tube to both the Wash Buffer Pump and the Elution Buffer Pump Note When setting the Pump Tube wear gloves CAUTION The front cover will not stay on the way to the upper limit If you release the front co...

Page 35: ...N Pump Tube or WASH Pump Tube for each pump Spare pump tube is available for either pump c Attach the pump cover Turn the rotor vertical position then re attach the pump cover to the retainer making sure that the drive head and drive socket are correctly mated Secure the pump cover in place with the original 2 screws Important Check the both end of the Pump Tube are placed the grooves Important Ch...

Page 36: ... tabs of the Buffer Stand in the slits on the QuickGene 810 2 Set empty conical tubes into the Buffer Stand Set two conical tubes for elution buffer and wash buffer When using standard conical tubes attach the adapter to the Buffer Stand 3 Set the Discharge Tray to the Buffer Stand To Wash Buffer Pump To Elution Buffer Pump Note When the carrier is on the left and it is difficult to attach the Buf...

Page 37: ...le Tube Cap Connect the Pump Tube for the Elution Buffer Pump lower blue and the blue Tube Cap Setup the Holder Carriage The following explains how to install the Holder Carriage 1 Attach the Holder Stand to the QuickGene 810 Insert the tabs of the Holder Stand in the slits on the QuickGene 810 2 Set Tube Holder and Cartridge Holder in the Holder Carriage Tube Cap Blue Tube Cap Purple Tube Cap Blu...

Page 38: ...e front cover 1 6 Checking QuickGene 810 Operation This section explains how to check the operation of QuickGene 810 After all the parts are installed check the operation of QuickGene 810 To check the operation use DNase RNase free water instead of buffers and dissolved samples and perform the same operation as the normal extraction operation For the extraction operation refer to 3 Operation page ...

Page 39: ...tting unit The buffers wash buffer elution buffer are set here The buffers set are sent to the discharge unit through the pump unit Pump unit Sends the buffers wash buffer elution buffer to the pressure unit according to the extraction program Carrier unit Puts pressurized air and buffers wash buffer elution buffer into the cartridge and performs the extraction process binding washing elution Samp...

Page 40: ...f the QuickGene 810 The QuickGene 810 provides the following safety mechanisms Front cover Earth leakage breaker LCD Lamp LED and buzzer Front cover When the front cover is open operations such as extraction processes cannot be performed Also if the front cover is opened during the extraction process it will be temporarily interrupted However when the buffer pump is working the pump will continue ...

Page 41: ...as not started yet A total of seven modes can be memorized PROCESSING Working the extraction program COMPLETED The extraction operation is completed The extraction result will be displayed after showing this message for one second START SW RESTART Temporarily stops This message is displayed in the following cases While waiting for the additional process When opening the front cover during the extr...

Page 42: ... 2 081 EN61010 2 081 CE LV directive 2006 95 EC EMC directive 2004 108 EC FCC WARNING Changes or modifications not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide...

Page 43: ...st after the power is turned on the LCD displays the software version for a few seconds then changes the display to WAIT A MOMENT LCD displays extraction mode name when the initialization has been completed It takes about a minute for the initialize Turning off the power 1 Press the O side of the switch Wear latex gloves and a mask during any operation CAUTION Do not place any objects containing l...

Page 44: ...run use DNase RNase free water Lysate Samples For a test run use DNase RNase free water For the preparation of samples refer to each kit s handbook Conical tubes are used for elution buffer and wash buffer Refer to 1 5 Setup Parts page 9 Discharge 1 Check the QuickGene 810 has been cleaned For clean up procedure refer to 4 3 Cleaning the QuickGene 810 page 32 and 4 4 Cleaning the Parts page 33 2 T...

Page 45: ... the specified kit for each sample For details refer to the kit s handbook 3 4 Extraction Operations This section explains each operation before the start of a program and after the completion of a program Operations before the start Important Buffers can be used for a day in the conical tube inside the QuickGene 810 Note Do not touch the inner tube of Tube Cap In the case of contamination please ...

Page 46: ...ge 4 Set collection tubes and waste tubes in the Tube Holder 5 Set the Tube Holder into the Holder Carriage Align the tab and notch Note When the carrier is in the center and it is difficult to remove the Holder Carriage close the front cover and press the CR MOVE button Important Put the tubes into the Tube Holder from the left without space Grip handles and lift to take out Holder Carriage Tube ...

Page 47: ... rests firmly on the metal shelf and two pins are placed in the holes Setup the Holder Carriage page 13 10 Close the front cover 11 Select an extraction mode Press the MODE button several times until the target extraction mode appears Important Insert the cartridges into the cartridge holder to the bottom of the holes Set cartridges in the order of the number on the Tube Holder If they are not in ...

Page 48: ...layed in the LCD If the front cover is opened the extraction process will be halted To continue the process refer to 3 5 Operations to Restart Program from Pause page 28 To quit the process refer to 3 6 Operations to Stop Extraction Process page 29 In some extraction modes operations are required during processing For details refer to TBD Display Meaning check Successfully extracted hyphen Extract...

Page 49: ... dispense a reagent into the cartridges 4 Set the Holder Carriage into the QuickGene 810 After setting it check the following Check that the Cartridge Holder is firmly in place Check that the Tube Holder rests firmly on the metal shelf and two pins are placed in the holes Setup the Holder Carriage page 13 5 Close the front cover 6 Press the START button QuickGene 810 re starts the program from the...

Page 50: ...ay to the upper limit If you release the front cover while opening it it may cause injury or device damage Note Take the handle of the Tube Holder After taking off the Holder Carriage from the QuickGene 810 the Tube Holder cannot be taken out of the the Holder Carriage Therefore take only the Tube Holder out of the QuickGene 810 If you took off the Holder Carriage from the QuickGene 810 take off t...

Page 51: ... Turn off the power of QuickGene 810 Dispose of the remaining buffer Clean the dirty parts Refer to 4 Maintenance page 31 for maintenance details Note When the carrier is in the center and it is difficult to remove the Holder Carriage close the front cover and press the CR MOVE button Note When the carrier is on the left and it is difficult to remove the Discharge Tray close the front cover and pr...

Page 52: ...restarts from the position where the process was paused Operations such as discharge may be performed even without cartridges or tubes which may result in liquid spill Process status Operations when opening the front cover Operations at restart During initialization All operations stop The pressure head returns to the original position then the operations restart from the paused position During bi...

Page 53: ...action operation check and follow the following Execute discharge first Please execute discharge first This operation discharges the old wash buffer elution buffer and air remaining in the tubes and refills new wash buffer and elution buffer After the discharge operation dispose of the liquid collected in the Discharge Tray Discharge page 20 Follow the setting order and set them correctly When set...

Page 54: ...in the correct positions Insert the cartridges and tubes starting from the left in order If they are not in order a spill of buffer and or dissolved sample may occur This results in an extraction failure and buffer and or dissolved sample waste 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8 Cartridge Holder Tube Holder OK OK OK NG ...

Page 55: ...ickGene 810 and clean the inside with ethanol Tasks required when the device is not used for a week or more If the QuickGene 810 will not be used for a week or more after the operation perform the following procedures 1 Put DNase RNase free water to both new conical tubes for wash buffer and elution buffer and perform the discharge operation For the discharge operation refer to Discharge page 20 2...

Page 56: ...disconnect the power plug from the outlet Otherwise it may cause an electric shock Parts to clean Cleaning methods Pressure Head Wipe with a Kimwipe or paper tissue soaked with ethanol QuickGene 810 outside Remove the dust lightly with a dry soft cloth QuickGene 810 inside Wipe with a Kimwipe or paper tissue soaked with 0 5 sodium hypochlorite solution Remove the dust lightly with a dry soft cloth...

Page 57: ...s needed When purchasing them contact the Customer Center Parts Cleaning methods Holder Carriage Clean the parts using one of the following methods 1 Clean with neutral detergent and then wash out with distilled water Next dip into 3 hydrogen peroxide solution at room temperature for 10 minutes Lastly wash the solution out with DEPC water and dry the parts 2 Soak in a 0 1N sodium hydroxide 0 1 EDT...

Page 58: ...ress the O side of the switch 4 Open the front cover Hold the handle and open the front cover until the upper limit is reached 5 Take off both the tube on the device side and the Tube Cap from the Pump Tube Take off both tube caps purple and blue by turning the connection points as shown in the figure 6 Take off the Buffer Stand from the QuickGene 810 7 Detach the pump cover Loosen and remove the ...

Page 59: ... Pump Cover by rotating the rotor Adjust the rotor to its original vertical position Use the Pump Tube as labeled ELUTION Pump Tube or WASH Pump Tube for each pump Spare pump tube is available for either pump After this the procedure is the same as in How to install the Pump Tube For details refer to Setup the Pump Tube page 10 Important Check the both end of the Pump Tube are placed the grooves N...

Page 60: ...1 2 6 3 4 5 7 4 Maintenance 36 QuickGene 810 Operation Manual Rev 1 7 ...

Page 61: ...e user s responsibility to change the parameters In EXPERT mode there are some crucial parameters Therefore you must not change any parameters in EXPERT mode without our guidance Display sequence LCD message Setting range Unit Mode name The value displays the default DNA WHOLE BLOOD RNA CELL RNA CELL PLUS DNA TISSUE RNA TISSUE RNA TISSUE PLUS PLASMID 1 BIND PEAK 50 160 kpa 120 120 120 120 120 120 ...

Page 62: ...BDR Sets the fifth wash buffer discharge rate Can be specified between 250 750µl in increments of 50l 9 WASH DIP TM 0 WASH DIP TM 510 Wash buffer immersion management timer Sets the wash buffer immersion management time Can be specified between 0 510 sec in increments of 2 seconds 10 WAS2 WAIT T 0 WAS2 WAIT T 255 Waiting time Sets the time to wait for shifting to wash buffer discharges in the seco...

Page 63: ...ely When changing other items repeat steps 3 and 4 18 ELUT VOL 50 ELUT VOL 400 elution buffer discharge rate Sets the elution buffer discharge rate Can be specified between 50 400l in increments of 50l 19 ELUT PEAK 50 ELUT PEAK 160 elution buffer peak pressure Sets the peak pressure during pressure application Judgment criteria is the pressure before pressure application the pressure set in this i...

Page 64: ...st even if the power is turned off When the LCD displays as shown in the figure at the left the changed settings are saved and the setup mode ends Important To cancel the saving parameters press the STOP or RESET button The setup mode ends without saving the changed parameters SETUP WRITING Display on operation panel Save the settings SETUP COMPLETED DNA WHOLE BLOOD Exit setup mode and display cur...

Page 65: ... 7 12 WASH PEAK 50 160 kpa 110 110 110 110 110 110 110 13 WASH UP TIME 1 50 sec 6 6 6 6 6 6 6 14 WASH RETRY 50 160 kpa 140 140 140 140 140 140 140 15 WASH LOWER 3 100 kpa 70 70 70 70 70 70 70 16 WASH DOWN TM 1 99 sec 15 15 15 15 15 15 15 17 WASH R DN T 1 100 sec 50 50 50 50 50 50 50 18 WASH FALL 5 60 kpa 60 60 60 60 60 60 60 19 WASH VOL1 250 750 µl 750 500 500 750 750 750 750 20 WASH VOL2 250 750 ...

Page 66: ...50 750 750 750 39 WAS2 VOL5 250 750 µl 750 500 500 750 750 750 750 40 ELUT VOL 50 400 µl 200 100 100 200 100 100 50 41 ELUT COUNT 0 5 time s 1 1 1 1 1 1 1 42 ELUT SPEED 1 12 7 7 7 7 7 7 7 43 ELUT PEAK 50 160 kpa 100 100 100 100 100 100 100 44 ELUT UP TIME 1 50 sec 4 4 4 4 4 4 4 45 ELUT RETRY 50 160 kpa 140 140 140 140 140 140 140 46 ELUT LOWER 3 100 kpa 65 65 65 65 65 65 65 47 ELUT DOWN TM 1 99 se...

Page 67: ...RENAME mode starts 5 Press the or button several times until the extraction mode name to be applied is displayed on the LCD Important In RENAME mode only extraction mode names are changed without changing parameter values of the corresponding modes Display sequence Extraction mode name Display sequence Extraction mode name 1 DNA WHOLE BLOOD Default 19 DNA PLANT 2 RNA CELL Default 20 RNA PLANT 3 RN...

Page 68: ... name is saved and the mode returns to the USER mode Important When not saving the new name press the button The LCD display changes to the RENAME MODE Y without saving the new name To cancel the new name press the STOP or RESET button The setup mode ends without saving the new name and the current extraction mode will be displayed Display on operation panel Extraction mode name before change RENA...

Page 69: ...stopped in the middle of the operation Did you open the front cover during the operation It sometimes stops for time adjustment during extraction work During extraction work While the operation panel displays PROCESSING do not open the front cover If you opened the front cover during the operation and it stopped the extraction work refer to 3 5 Operations to Restart Program from Pause page 28 The ...

Page 70: ... pump become unfastened from the device Contact your local representative Phenomenon Cause Appropriate action Message Contents Appropriate action MT ER CR Carrier motor or air pump has failed Contact your local representative The message CR HD TB and WS may appear at the same time depending on failure type MT ER HD The nozzle head motor has failed MT ER TB Table motor or lift motor has failed MT E...

Page 71: ...rent carrying conductors ground UL CSA Approved NEMA 5 15P parallel blade Rated 10A 125Vac UL CSA Approved 200 240 Vac IEC60320 C 22 Rated 10A 250Vac UL CSA Approved Type SJT No 18 AWG Min 3 Conductors Single phase 2 current carrying conductors ground UL CSA Approved NEMA 6 15P tandem blade Rated 10 A 250 Vac UL CSA Approved Europe 2 100 240 Vac IEC60320 C 22 Rated 10A 250Vac 1 CENELEC 0C 3X1 O sq...

Page 72: ...arts please contact to local representative Name Holder Carriage Name Cartridge Holder Name Tube Holder Name Buffer Stand Name Adapter Name Discharge Tray Name Holder Stand Name Pump Tube Name Tube Cap Large Blue Name Tube Cap Large Purple Name Tube Cap Small Blue Name Tube Rack 4pcs Name QG810 Carriage Set ...

Page 73: ...quivalent box A 6 Other Information Information for traceability in Europe If you would like to order spare parts please contact to local representative This is a class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures WARNING When carrying the QuickGene 810 disconnect the plug from the outlet first Watch ...

Page 74: ...rand FUJIFILM Compiler Dr Michael Kaling Authorized representative in Europe FUJIFILM Europe GmbH Heesenstrasse 31 40549 Dusseldorf GERMANY Type of Equipment Nucleic Acid Isolation System Model No QuickGene 810 From S N H0901001 Parts No PA03384 B021 We herewith declare that the above mentioned products meet the provisions of the following EC Council Directives and Standards Place JAPAN Yoshihiro ...

Page 75: ...㩷㪝㪊㪎㪉㪞㪎㪎㪇㪇㪇㪈 ᇐ ᛆⲬ 㬍 㬍 㬍 㬍 㬍 㬍 㪧㪆㪥㩷㪑㩷㪝㪊㪍㪉㪞㪎㪎㪇㪇㪇㪈 ᜬౕ 䂾 䂾 䂾 䂾 䂾 䂾 㪧㪆㪥㩷㪑㩷㪝㪏㪇㪉㪞㪎㪎㪇㪇㪇㪊 䂾 䂾 䂾 䂾 䂾 䂾 㪧㪆㪥㩷㪑㩷㪝㪊㪏㪌㪞㪎㪎㪇㪇㪇㪈 䖰ᴉ 䋨ᄢ 㪱 䋩 䂾 䂾 䂾 䂾 䂾 䂾 㪧㪆㪥㩷㪑㩷㪝㪊㪈㪎㪞㪎㪎㪇㪇㪇㪈 䖰ᴉ 䋨ᄢ 䋩 䂾 䂾 䂾 䂾 䂾 䂾 㪧㪆㪥㩷㪑㩷㪝㪊㪈㪎㪞㪎㪎㪇㪇㪇㪉 䖰ᴉ 䋨ዊ 㪱 䋩 䂾 䂾 䂾 䂾 䂾 䂾 㪧㪆㪥㩷㪑㩷㪝㪊㪈㪎㪞㪎㪎㪇㪇㪇㪊 䆩 ᛆⲬ 䂾 䂾 䂾 䂾 䂾 䂾 㪧㪆㪥㩷㪑㩷㪝㪏㪇㪉㪞㪎㪎㪇㪇㪇㪋 Ḯ 㓚䋨ᣣᧄ䋩 㬍 䂾 䂾 䂾 䂾 䂾 㪧㪆㪥㩷㪑㩷㪝㪈㪊㪍㪞㪎㪎㪇㪇㪇㪈 Ḯ 㓚䋨ർ ᵮ䋩 㬍 䂾 䂾 䂾 䂾 䂾 㪧㪆㪥㩷㪑㩷㪝㪈㪊㪍㪞㪎㪎㪇㪇㪇㪉 Ḯ 㓚䋨 ᵮ䋩 㬍 䂾 䂾 䂾 䂾 䂾 㪧㪆㪥㩷㪑㩷㪝㪈㪊㪍㪞㪎㪎㪇㪇㪇㪊 QG810ಽᨆḋຠ ᅃ 䂾 䂾 䂾 䂾 䂾...

Page 76: ......

Page 77: ...information in this manual Contents of this manual are not to be reproduced without permission from FUJIFILM Corporation Manuals with missing or wrongly collated pages will be replaced free of charge Nucleic Acid Isolation System QuickGene 810 Operation Manual Date of issuance July 2010 FUJIFILM Corporation Printed in Japan December 2012 KURABO INDUSTRIES LTD KURABO INDUSTRIES KURABO INDUSTRIES ...

Page 78: ...0 E Y10060557 LS 1007 1 KURABO INDUSTRIES LTD Bio Medical Department Kurabo Neyagawa Techno Center 3F 14 5 Shimokida Cho Neyagawa Osaka 572 0823 Japan TEL 81 72 820 3079 FAX 81 72 820 3095 URL http www kurabo co jp bio English ...

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