Kurabo QuickGene-810, Operation Manual

Page: 61 / 78

QuickGene-810 Operation Manual
Rev. 1.7
37
5 How to Set Parameters
1
3
2
5
4
6
7
5
How to Set Parameters
This chapter explains how to set parameters.
"Set Parameters" means to change the values (such as buffer volume, number of discharges, etc.) for each
extraction mode.
The name of each extraction mode can be selected from 35 names.
The parameters set in the QuickGene-810 are stored in 7 separate memory areas. Therefore, even if an
extraction mode name is changed, the parameter values will not be changed.
•
→
"5.2 How to Set/Change Parameters" (page 39)
•
→
"5.4 Changing Extraction Mode Names" (page 43)
5.1 Parameters
Settings of each parameter can be changed from the setup mode.
Some parameters are changed from the normal setup mode (USER mode) and others are changed from the
EXPERT mode.
The following table shows the display sequence, setting range, default value, etc. for each parameter in
USER mode.
Important: • The setup is used to change the parameters, according to new protocol of
the kit, etc. Please check the parameters for each kit's Handbook.
• In the case of wrong value for parameters, it will cause buffer spill or
sample extraction failure.
It is the user's responsibility to change the parameters.
• In EXPERT mode, there are some crucial parameters. Therefore, you must
not change any parameters in EXPERT mode without our guidance.
Display
sequence
LCD message
Setting
range
Unit
Mode name (The value displays the default)
DNA
WHOLE
BLOOD
RNA
CELL
RNA
CELL
PLUS
DNA
TISSUE
RNA
TISSUE
RNA
TISSUE
PLUS
PLASMID
1 BIND PEAK 50 - 160 kpa 120 120 120 120 120 120 120
2 WASH COUNT 0 - 5 time(s) 3 3 1 3 3 1 2
3 WASH PEAK 50 - 160 kpa 110 110 110 110 110 110 110
4 WASH VOL1 250 - 750 µl 750 500 500 750 750 750 750
5 WASH VOL2 250 - 750 µl 750 500 500 750 750 750 750
6 WASH VOL3 250 - 750 µl 750 500 500 750 750 750 750
7 WASH VOL4 250 - 750 µl 750 500 500 750 750 750 750
8 WASH VOL5 250 - 750 µl 750 500 500 750 750 750 750
9 WASH DIP TM 0 - 510 sec 0 150 150 0 150 150 0
10 WAS2 WAIT T 0 - 255 min 0 0 5 0 0 5 0
11 WAS2 COUNT 0 - 5 time(s) 0 0 2 0 0 2 0
12 WAS2 PEAK 50 - 160 kpa 110 110 110 110 110 110 110
13 WAS2 VOL1 250 - 750 µl 750 500 500 750 750 750 750
14 WAS2 VOL2 250 - 750 µl 750 500 500 750 750 750 750
15 WAS2 VOL3 250 - 750 µl 750 500 500 750 750 750 750
16 WAS2 VOL4 250 - 750 µl 750 500 500 750 750 750 750
17 WAS2 VOL5 250 - 750 µl 750 500 500 750 750 750 750
18 ELUT VOL 50 - 400 µl 200 100 100 200 100 100 50
19 ELUT PEAK 50 - 160 kpa 100 100 100 100 100 100 100
20 ELUT DIP TM 0 - 510 time(s) 0 30 30 90 30 30 0