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Mass spectrometer

Instructions

Document No. V6695

HPLC

4000 MiD®

Summary of Contents for 4000 MiD

Page 1: ...Mass spectrometer Instructions Document No V6695 HPLC 4000 MiD...

Page 2: ...14163 Berlin Phone 49 30 809727 0 Fax 49 30 8015010 Internet www knauer net E Mail info knauer net Version information Article number V6695 Version number 1 0 Last update 2018 08 21 Copyright This doc...

Page 3: ...anel 12 4 Scope of delivery 13 5 Unpacking and setup 14 5 1 Site requirements and storage conditions 14 5 2 Power supply 14 5 3 Power cords 14 5 4 The MiD and associated parts 15 5 5 Setting up the Mi...

Page 4: ...Removing and replacing the spraychip 33 9 3 Cleaning the spraychip 34 9 3 1 Cleaning the Spraychip with DFI 34 9 3 2 Cleaning the Spraychip with SFI 34 9 4 Liquid flow rate measurement 35 9 5 Spraychi...

Page 5: ...ng operation and maintenance of the MiD Failure to comply with the warning instructions violates safety standards associated with the proper use of the system and its design KNAUER assumes no liabilit...

Page 6: ...emical This symbol with the Warning notice denotes corrosive chemicals A corrosive chemical may be present Exposure may result in serious injury Use appropriate skin protection Note A Note indicates i...

Page 7: ...s notices d avertissement sont aussi associ es avec des pictogrammes repr sentant des dangers sp cifiques Attention possibilit de choc electriqu Ce pictogramme d note la possibilit de choc lec trique...

Page 8: ...quipement est utilis d une mani re non sp cifi e par le fabricant la protection fournie par l quipement peut tre alt r e Caution The MiD should only be used with accessories that meet the manufacture...

Page 9: ...n analysing hazardous chemicals Avertissement Les gaz d chappement de la pompe du MiD doit tre connect un syst me d extraction appropri Une extraction ad quate doit galement tre fournie la zone micros...

Page 10: ...ges voltage fluctuations and flicker in public low voltage supply systems for equipment with rated current 16 A per phase and not subject to conditional connection ICES 003 2004 Spectrum Management an...

Page 11: ...ifies the original product or installs additional compo nents Avertissement La d claration du fabricant est annul e si l uti lisateur modifie l origine du produit ou installe des composants suppl ment...

Page 12: ...ribed in these Instructions Avertissement NE PAS essayer de faire fonctionner l appareil avec les couvercles enlev s Des hautes tensions sont pr sentes lorsque l appareil est en fonctionnement NE PAS...

Page 13: ...mall sample volumes for example nanolitres over long periods of time Very low solvent consumption and minimum waste when coupled to a nano LC Lower voltages are required to provide efficient electrosp...

Page 14: ...asses through an emitter or capillary tip at a flow rate of 300 1000 nL min The voltages and flow rates used in microspray are lower than for convention al electrospray ionisation This means that lowe...

Page 15: ...1 They are used to indicate the current operational status of the instrument the LEDs are shown in detail in Fig 2 Fig 2 LED status indicators The LEDs indicate the following statuses Red Indicates t...

Page 16: ...panel of the MiD These ports include terminals and connectors for the gas supply vacuum exhaust system and computer peripherals Refer to the labels listed in the table below when connecting the MiD an...

Page 17: ...Only use original parts and accessories made by KNAUER or a com pany authorized by KNAUER 4000 MiD MiDas Starter kit Mains leads 2 in total PTFE tubing form Nitrogen input Exhaust Silencer Split flow...

Page 18: ...behind the instrument to un plug the power cord Attention Lors de l installation ou l utilisation de l instrument vous devez vous assurer qu il ya suffisamment d espace derri re l appa reil pour d bra...

Page 19: ...o change Caution All items listed must only be supplied by the manufac turer or an agent thereof Use of alternative parts may lead to improper operation of the MiD or failure to comply with safety or...

Page 20: ...ate and dust cap 5 5 Setting up the MiD Fig 4 shows a typical configuration of the 4000 MiD Fig 4 The 4000 MiD Caution Allow sufficient spacing around the MiD for proper cooling and for the connection...

Page 21: ...s les r gles de sant et les r gles de s curit du pays Caution The maximum weight that can be supported by the MiD is 60 kg Attention Le poids maximum qui peut tre pris en charge par le MiD est de 60 k...

Page 22: ...itable length of clean PTFE tubing from the gas supply to the Nebuliser Gas Port A 6 mm tubing can be used Note If replacing or refitting the gas supply tubing it is recommended that the end of the li...

Page 23: ...r and the ion guide chamber housing the ion guide Fig 6 The SFD assembly 5 6 The spraychip flange The spraychip flange is used to connect the MiD s microspray source to the SFD and provides the nebuli...

Page 24: ...vac chip needs to be cleaned or replaced see 8 7 Removing and replacing the vac chip on page 42 for further details To fit or remove the vac chip assembly follow the subsequent steps The vac chip ass...

Page 25: ...Fig 9 The vac chip assembly being installed on the MiD 3 If excessive resistance is felt when fitting the assembly to the SFD stop immediately remove the assembly and retry step 2 4 Once positioned on...

Page 26: ...ntained within the spraychip assembly 5 8 The spraychip assembly The spraychip assembly consists of the spraychip and a mounting inter face there are three available components The spraychip 200 Split...

Page 27: ...rt Fig 11 The Split Flow Interface SFI To assemble the spraychip to the main body of the SFI use the following procedure 1 Place the PEEK screw over the metal collar as shown in Fig 11 2 Unscrew the g...

Page 28: ...ut flow rate and liquid composition changes during a gradient Flow rates can be measured using the flow meter assembly see sec tion 8 4 Liquid flow rate measurement on page 35 For a fixed length decre...

Page 29: ...itting a spraychip assembly to the MiD a nitrogen flush of the interface must be initialised Open the software and click the nitro gen flush button after a few seconds a flow of nitrogen gas should be...

Page 30: ...e door in the front cover The door has two positions as shown in Fig 16 When the door is shut the safety interlock is off and high voltages can be applied his is indicated in the Status bar When the d...

Page 31: ...from the rear of the instrument of 70dB Attention Une fois install et en fonctionnement normal ce produit a une mission de pression acoustique la position de l op rateur et de 1 m de l arri re de l in...

Page 32: ...firm before the vent cycle starts 6 2 Shutting down the MiD The following section details the basic procedures for putting the MiD into standby venting the system and powering down the system 6 2 1 En...

Page 33: ...nctionality of the device 7 2 Operation Qualification OQ The Operation Qualification includes an extensive functionality test accor ding to KNAUER standard OQ documents The Operation Qualification is...

Page 34: ...using the appropriate calibration solution directly from a syringe pump 1 If the MiD is turned off switch it on and pump the instrument down as described in the section MiD system pump down 2 Fill the...

Page 35: ...maintenance procedures the door has to be ope ned with the safety interlock engaged If in any doubt power down the instrument see the section 6 2 3 Shutting down the MiD on page 28 and perform the ma...

Page 36: ...dures detailed in this chapter Warning Handling toxic flammable and hazardous chemicals can lead to health and safety risks Avertissement La manipulation de produits chimiques toxiques inflammables et...

Page 37: ...assembly see Fig 32 for reference Fig 18 The Split Flow Interface SFI 7 Carefully unscrew and remove the spraychip from the DFI SFI 8 Carefully screw a new or cleaned spraychip into the assembly Note...

Page 38: ...for use Note If an increased delay is observed on the split flow inter face SFI when using the cleaned spraychip then attempt to clean again In the event that this delay continues to be unac ceptable...

Page 39: ...chip assembly is measured This can be performed on both the DFI and SFI To measure the liquid flow rate the flow meter assembly Fig 33 will be required Fig 19 Flow meter assembly 1 Remove the spraychi...

Page 40: ...w when a custom split ratio is created for an SFI see 5 8 1 The Split Flow Interface SFI on page 23 If the measured flow is incorrect the user can simply change the length or diameter of the waste lin...

Page 41: ...replace the low flow tubing located inside the DFI see 8 6 Spraychip assembly maintenance on page 38 9 5 2 Cleaning the Split Flow Interface The following procedure should be used to clean the SFI 1 F...

Page 42: ...flow tubing should be replaced The following sections detail the procedures for replacing the tubing in both the DFI and SFI assemblies The SFI DFI tubing kit A66919 will be re quired for the followin...

Page 43: ...removed 7 Unscrew the fingertight nuts from the input and reducing unions and remove the low flow tube 8 To fit a new low flow tube first slide the tube through one of the fingertight nuts 9 Fit the n...

Page 44: ...e initial problem persists and or you have any queries please contact KNAUER 9 6 2 Replacing the Split Flow Interface tubing The following procedure should be used to replace the tubing inside the SFI...

Page 45: ...e the screw retaining the tee Fig 42 Fig 28 SFI splitting tee and retaining screw 8 Remove the tee reducing union and tubing from the SFI using the plastic tweezers A66911 Fig 43 Fig 29 SFI with split...

Page 46: ...ig 41 18 Refit the metal collar and PEEK screw by reversing steps 3 6 The SFI low flow tube has been successfully changed Fit either a spray chip 200 or measure the flow rate Liquid flow rate measurem...

Page 47: ...he vac chip flange with five vacuum screws The vac chip is located under this assembly Fig 30 The vac chip and tube lens assembly 12 Using the Phillips head screwdriver partially unscrew the five vacu...

Page 48: ...e Do not over tighten the vacuum screws and do not continue to tighten screws if any resistance is felt in this case remove the assembly and retry Caution The vacuum screws have been specially made fo...

Page 49: ...th of the tube 5 Slowly turn the test tube until vertical allowing the vac chip to slide slowly to the bottom of the tube 6 Pour enough HPLC grade water into the test tube to cover the vac chip 7 Plac...

Page 50: ...auf qu elles ont t fournies par le fabricant Caution The vac chip is fragile Always handle it with extreme caution Attention Le vac chip est fragile Toujours manipuler avec une extr me prudence 1 Clea...

Page 51: ...owing table details some basic symptoms possible causes and potential solutions for some basic issues with the 4000 MiD Symptom Possible Cause Remedy Cannot apply any voltages to the spraychip assembl...

Page 52: ...flow from the syringe pump LC to the spraychip assembly Capillary tip may be clogged Change the spraychip 200 see chapter 8 2 on page 33 A voltage cannot be applied to the spraychip assembly because t...

Page 53: ...uations increase under fixed condi tions Capillary tip may be dull or clogged Replace the spraychip 200 see chapter 8 2 on page 33 A leak in the tubing Check all fluidic connec tions Ions are in the w...

Page 54: ...to 2000 m Temperature Operation 15 30 C Storage 10 50 C Relative humidity Operation 40 80 Pollution Degree 2 Normally only non conductive pollution occurs Occasionally however a temporary conductivit...

Page 55: ...aste line increases the spraychip flow rate For a fixed diameter increasing the length of the waste line increases the spraychip flow rate Changing the diameter of the waste line has a greater effect...

Page 56: ...tion 1 If the inner diameter of your desired waste tube is 254 m then Note It is always better to start with a longer piece of tubing at least 10 cm longer so that if the low flow rate is too high the...

Page 57: ...ay D type male socket and is shown in more detail in Fig 49 Fig 35 Auxiliary I O port The port contains two optically isolated digital inputs and two optically isolated digital outputs The digital inp...

Page 58: ...h an open ended cable for development purposes Wires will either be coloured or numbered as appropriate identifiers Auxiliary I O port pin assignments Pin Number Signal Name Description Comments 1 5 V...

Page 59: ...n supplied with an open ended cable examples include triggering from active and passive sources All terminations not made to the MiD should be made in accordance with the relevant manufacturer s instr...

Page 60: ...MiD The manufacturer is not responsible for any damage to external instrumentation Attention Lors de la fixation du MiD la source de d clenche ment passif l utilisateur doit s assurer que l instrument...

Page 61: ...57 Appendices 4000 MiD Instructions V6695...

Page 62: ...tions online https www knauer net en Support User manuals KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Phone Fax E Mail Internet 49 30 809727 0 49 30 8015010 info knauer net www kn...

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