5 - 4
(11) Set the pump flow rate to 0.2mL/min L-2100 .
Set the pump flow rate to 1.0mL/min L-2130 .
(12) Set the detector wavelength to 250 nm. Set the detector
time constant to 0.1 s.
(13) Set the following conditions in the data processing unit.
SAMPLE PERIOD : 100 ms
STOP TIME
: 3 min
Check the reproducibility of the injection volume in the following
procedure.
(1) Press the
PROG
key and set the following conditions.
TIME
FLOW EVENT [--]
0.0
1.000
1
Setting can be completed by pressing the
ESC
key.
(2) Prepare 60% methanol for the eluent and detergent.
Wait until the pump, detector, etc. are warmed up and the
baseline stabilizes.
(3) Place a vial containing at least 1 mL of standard sample in
rack position No. 1 and set the rack in the autosampler.
(5) Press the
START
key of autosampler to start analysis.
The analysis is carried out 6 times. The operating
conditions during analysis are indicated on the display.
Ensure beforehand that the room temperature will not
change suddenly during analysis.
(6) Data Check
Determine the coefficient of variation (RSD) of peak area
by the following calculations.
Mean value
n
xi
x
n
1
i
∑
=
=
Standard deviation
1
n
)
x
xi
(
SD
n
1
i
2
−
−
=
∑
=
5.1.3 Checking the
Flow rate
stability
