Hitachi L-2100 Instruction Manual Download Page 68

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4.3.4

Flow Calibration

Set a parameter of flow rate calibration.

Monitor screen

UTLTY

UTILITY CONDITION=1 UNIT=2 FLUSH=

1 EVENT 4 LINK= OTHERS=6

Select CONDITION.

CONDITION SET Z-ADJ=1 MOTOR-CTRL=2

PURGE=3, CALIBRATION=4

Select CALIBRATION.

CALIBRATION FLOW=1 PRESS=2

Select FLOW.

FLOW CALIB. 0.97 – 1.03

1.000

Enter a parameter o flow rate calibration

Monitor screen

Summary of Contents for L-2100

Page 2: ...nces adverse to safety Carefully read this instruction manual before attempting operation For proper use of the pump please acquaint yourself with it ABOUT THIS MANUAL The operating procedures and maintenance checkup instructions for the L 2100 2130 pump are described in this manual First of all read IMPORTANT and SAFETY SUMMARY at the beginning of this manual for ensuring safety in operation of t...

Page 3: ... 2 ...

Page 4: ...a of the radio television receiver Increase separation between the instrument and the radio TV receiver Connect the instrument to an outlet on a circuit different from that to which the radio TV receiver is connected 2 Possible Electromagnetic Wave Interference Affecting This Instrument Where this instrument is used near an intense electromagnetic source interference noise may be given to the inst...

Page 5: ... Warranty Any parts which prove to be defective in design or workmanship during the warranty period will be repaired adjusted or replaced without charge A substitute part may be used for repair or replacement with an equivalent product may be made instead of repair Such system components as a personal computer and printer to be updated frequently for improvement may not be available in original ve...

Page 6: ...to improper handling or maintenance by the user g Failure due to maintenance or repair by a service agent not approved or authorized by Hitachi High Technologies Corporation h After disposal of this instrument or after its resale without Hitachi High Technologies Corporation approval i Failure due to relocation or transport after initial installation j Failure due to disassembly modification or re...

Page 7: ... THE MANUFACTURER BE LIABLE FOR ANY DIRECT INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY NATURE OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT NO ORAL OR WRITTEN INFORMATION OR ADVICE GIVEN BY THE MANUFACTURER ITS DEALERS DISTRIBUTORS AGENTS OR EMPLOYEES SHALL CREATE A WARRANTY OR IN ANY WAY INCREASE THE SCOPE OF THIS WARRANTY 6 Warranty Card The warranty card wi...

Page 8: ...onsible for following relevant legal standards and regulations in the handling storage and discarding of chemicals and samples used in analytical operations of the instrument 2 Reagents standard solutions and accuracy control samples shall be handled stored and discarded as instructed by the respective suppliers 2 Notice on This Instruction Manual 1 The information contained in this manual is subj...

Page 9: ...for detecting a signal of a separated component of the sample In addition a degasser for deaerating an eluent a gradient device for varying a mixing ratio of eluents with time and other optional devices are incorporated in the system 1 1 Isocratic System The isocratic system has a simple configuration in which single solvent analysis is performed 1 1 Model L 2100 2130 pump Model L 2300 column oven...

Page 10: ...ple is injected from the autosampler 3 The injected sample is separated through the column which is maintained at a constant temperature by the column oven 4 Each component separated from the sample is then detected as a signal by the detector 1 1 Isocratic System ...

Page 11: ...pressure unit the eluents are mixed at a pre specified ratio 3 Through the mixer the mixture eluent is delivered 4 A sample is injected by the autosampler 5 The injected sample is separated through the column which is maintained at a constant temperature by the column oven 6 Each component separated from the sample is detected as a signal by the detector 1 2 Model L 2100 2130 pump Model L 2300 col...

Page 12: ...re mixed in the high pressure gradient section Through the mixer the mixture eluent is delivered 4 A sample is injected by the autosampler 5 The injected sample is separated through the column which is maintained at a constant temperature by the column oven 6 Each component separated from the sample is detected as a signal by the detector 1 3 High Pressure Gradient System Eluent Drain Model L 2100...

Page 13: ...d place them on a sturdy level desk or bench NOTICE Installation of this instrument shall be carried out by or under supervision of qualified service personnel of Hitachi High Technologies Corporation or its authorized service agent Before installation of this instrument the customer shall make preparations for satisfying the installation requirements in accordance with this manual When relocation...

Page 14: ...y c Free from vapors of solvents e g benzene thinner that could affect the paint coating on the instrument 4 Other Requirements a Not exposed to direct sunlight Exposure to direct sunlight could result in deteriorated performance or discoloration of the instrument Avoid installing the instrument near a window of the laboratory room b Not exposed to drafts directly c Free from intense vibrations an...

Page 15: ...the performance of the instrument g No abrupt fluctuations in power voltage Fluctuations in power voltage could cause noise in analytical data h Do not connect the instrument to a power line used by an electric device that is not equipped with a noise suppressor circuit e g stirrer vibrator 2 2 ...

Page 16: ... 3 or higher should be provided WARNING Ground Properly to Prevent Electric Shock Hazard Be sure to use the power cable supplied with the instrument Use of a different power cable could result in an electric shock hazard This instrument is of a plug connected type specified in European Norm EN61010 1 so connect the power cable to a grounded three wire outlet If a grounded three wire outlet is not ...

Page 17: ...llation of the instrument the customer is requested to prepare necessary utilities referring to this manual When relocation of the instrument becomes necessary after initial installation delivery please notify your local Hitachi High Technologies sales representative or service office 2 4 Checking the Contents After unpacking check the contents of each case against the packing list attached to it ...

Page 18: ...em power supply unit is insufficient for the use of two detectors use an AC adapter suitable for each instrument 50 W AC adapter for the pump autosampler 100 W AC adapter for the detector The power cable from the system power supply unit and the AC adapter power cable should be connected to the power connector equipped on the left rear side of the instrument 2 5 1 Power Supply Unit 2 5 Assembly Re...

Page 19: ...0 pump connect the 50 W cable from the system power supply unit to the instrument power connector For the L 2400 UV detector connect the 100 W cable from the system power supply unit to the instrument power connector Model L 2100 2130 pump Model L 2100 pump Model L 2300 column oven Model L 2400 UV detector To AC power source System power supply nit 100 W cable 50 W cable 2 5 2 ...

Page 20: ... W cable from the system power supply unit to the instrument power connector In the L 2000 Series connect the e Line cable to the e Line connector equipped on the left rear of each instrument for instrument to instrument wiring connection 2 5 3 Wiring Arrangement Model L 2100 2130 pump Model L 2100 pump L 2300 column oven Model L 2400 UV detector To AC power source System power supply unit 100 W c...

Page 21: ...100 2130 pump Model L 2100 pump Model L 2300 column oven Model L 2400 UV detector System power supply unit e Line cable e Line cable e Line cable Model L 2100 2130 pump Model L 2100 pump Model L 2300 column oven Model L 2400 UV detector System power supply unit e Line cable e Line cable e Line cable ...

Page 22: ...r Provide tubing from the pump to the autosampler column and detector through the center piping section of the drain reservoir 2 5 4 Piping Arrangement Center Piping Section Run tubes from the pump to the autosampler column and detector Right Piping Section Run inlet tubing to the pump and waste solution tubing Drain reservoir 2 5 Assembly ...

Page 23: ...nnect port 2 of the injection valve to the column inlet Connect the column outlet to the inlet tube of the detector cell For washing solution provide connection to port 1 of the syringe valve of the autosampler through the degasser Inlet tube Eluent Column Injection valve Drain valve Detector cell Drain Model L 2100 pump Model L 2300 column oven Model L 2400 UV detector Model L 2100 2130 pump Wash...

Page 24: ...utosampler Connect port 2 of the injection valve to the column inlet Connect the column outlet to the inlet tube of the detector cell For washing solution provide connection to port 1 of the syringe valve of the autosampler through the degasser Inlet tube Eluent Column Injection valve Drain valve Detector cell Model L 2100 pump Model L 2300 column oven Model L 2400 UV detector system power supply ...

Page 25: ...e valve of the autosampler through the degasser Where the chromato data station is not used for controlling operations each module can be operated using the optional UI pad This section describes how to use the UI pad for operations The UI pad is provided with common control keys available for different modules and unique control keys for particular modules The functions of the common control keys...

Page 26: ...ned by pressing the ENT key Autosampler sequential program When the DEL key is pressed the relevant step number is deleted and is indicated This deletion can then be defined by pressing the ENT key CL Clears an input numeric value during input after a numeric value or the decimal point is entered before the ENT key is pressed The cursor is indicated at the top of the input part The previous input ...

Page 27: ...ues 3 Selection of a numeric value 4 Program setup window PROGRAM No 1 9 1 Parameter name Input range Input part LAMP CHANGE MM DD YYYY 1 12 Parameter name Input range Input part Input item TIME CONSTANT 0 1s 1 0 5s 2 2 0s 3 3 4 0s 4 8 0s 5 Parameter name Input part Selectable items NO R NO VIALS INJ VOL INJ VIAL S TIME 1 1 80 50 1 10 0 Input part Input item 2 5 5 ...

Page 28: ...re prompted to enter a correct numeric value Pressing the ESC key brings you back to the initial window monitor window If the ESC key is pressed during numeric key input the parameter being entered is discarded the previous numeric value is set NOTICE At power on a default number is indicated in each item selection part Pressing the CL key in the input part clears its contents The cursor is then b...

Page 29: ...2 17 2 Mounting the UI Pad a Remove the front panel from the instrument b Remove the metal lid from the instrument 2 5 5 Front panel Meal cover ...

Page 30: ...ible tube of the UI pad through the metal lid d Turn up the lid of the connector and insert the flexible tube of the UI pad as far as it will go UI pad Flexible tube Metal cover Connector Flexible tube UI pad 2 5 Assembly ...

Page 31: ...2 19 e Turn down the connector lid and mount the UI pad f Attach the metal lid dedicated for the UI pad 2 5 5 Flexible tube Metal cover UI pad Connector UI pad Flexible tube ...

Page 32: ...2 20 g Set the of the UI pad to the of the instrument h Mount the front panel dedicated for the UI pad on the instrument UI pad Tab Front panel 2 5 Assembly ...

Page 33: ...ument fastening can be made by shifting each wiring cover as shown below 1 Remove the retaining screws and detach the wiring cover 2 Shift the mounting position of each wiring cover 2 5 6 Instrument to Instrument Fastening 2 5 6 Wiring cover Retaining screw Wiring cover Retaining screw ...

Page 34: ...mp independently available at option LED There are 4 LEDs and each LED lights in a specific color for the determined indication Name color LED Status A green orange Green POWER ON as the pump A Orange Pump A error B green orange Green POWER ON as the pump B Orange Pump B error PURGE green Green Under purge PROG RUN green Green Under the time program running 3 1 1 Name and Function of Each Part Dra...

Page 35: ...or other units of the L 2000 series Ground terminals Three terminals for grounding the instrument I F board mounting IF board space Space for mounting the I F board required for connecting the PC data station and HPLC system 3 1 1 Wiring covers I F board mounting space Ground terminals Wiring covers Pressure value is output to the exterior Approx 49 MPa 1 V Pump can start by an external signal Pum...

Page 36: ...ed time of a time program TIMER No and Event code Pump can be stopped by specifying 00 for EVENT and buzzer can be rung by specifying 92 1 Becomes OFF when the pulse is output in the ON status 2 OFF has priority over ON 3 Contact output specification of each terminal Voltage 30 V DC max Current 100 mA max Output PRESS OUT Pressure value is output to the exterior 0 to 1 V DC 1 V Approx 49 MPa Appro...

Page 37: ...eed mode are available SET UP GLP Used for confidence level reporting PROG Selects generates edits or deletes a pump program Scroll up and down display START Starts the time program You can activate the start key only when the pump is at the initial mode STOP Stops the time program MANUAL Used for manual operation selection display and setting mobile phase mixing ratio FLOW P MAX P MIN B C D ESC L...

Page 38: ...reen Operation method of the monitor screen and example indications on it are shown below a 1st monitor screen This screen mainly indicates information about the pump main frame Indication Description TIME A B C D FLOW PRESS Elapsed time of analytical program execution Mixing ratio of liquid A Mixing ratio of liquid B Mixing ratio of liquid C Mixing ratio of liquid D Pump flow rate Pump pressure P...

Page 39: ... exceeds the specified upper limit the pump is forced to stop P MIN Indicates a specified lower limit of pressure If pressure decreases below the specified lower limit the pump is forced to stop c 3rd monitor screen Indication Description EVENT Indicates a state of the EVENT terminals 1 to 4 ON represents that the contact is closed and OFF represents that the contact is open PUMP Indicates a pump ...

Page 40: ...s OFF the 4th monitor screen will not appear 1 1st MONITOR screen TIME A B C D FLOW PRESS 0 0 100 0 0 0 0 000 0 0 1 2 2nd MONITOR screen P UNIT P MAX P MIN MPa 20 0 0 0 1 3 3rd MONITOR screen EVENT 1 2 3 4 PUMP FLUSH OFF OFF OFF OFF A Micro OFF 1 4 4th MONITOR screen LINK PROG STEP CYCLE ON 1 1 99 1 or ESCAPE ...

Page 41: ...IAL Programming mode status in which system waits for start MANUAL Operation is made under constant conditions without using program RUN Gradient mode is executed Fig 3 3 Operational Status Transition does not function unless the pump is on INITIAL START STOP MANUAL SET RUN MANUAL MANUAL SET START STOP INIT 3 2 Operation Status ...

Page 42: ...etention time using L 2200 autosampler Flow 0 2 mL min at constant room temperature of 20 for water h Pressure display accuracy 5 i Constant pressure liquid feed 2 0 to 39 2 MPa Flow range 0 to 1 25 mL min j Material of wetted parts SUS 316 ruby sapphire ceramic PTFE carbon impregnated PTFE k Display data using UI Pad Option Parameters Gradient monitor Elapsed time mixing ratio flow rate pressure ...

Page 43: ... temperature of 20 for water 2 uL min 0 01 0 10 mL min 1 0 39 2 MPa 2 0 101 5 000 mL min 1 0 39 2 MPa 2 5 001 8 000 mL min 1 0 19 6 MPa 4 8 001 9 999 mL min 2 0 19 6 MPa g Flow precision 0 075 RSD or 0 02min SD whichever is greater based on retention time using L 2200 autosampler Flow 1 0mL min at constant room temperature of 20 for water h Pressure display accuracy 5 i Constant pressure liquid fe...

Page 44: ...t input output terminals Event timer 4 channels n Communication e Line OUTPUT BUSY INPUT 1 GRADIENT START IN 2 ERROR IN o Pressure zero adjustment Possible p Ambient temperature 4 to 35 C condensation unallowable q Humidity 45 to 85 RH r Power supply 24V DC 21 A s Dimensions 260 W 500 D 235 H mm t Weight About 15 kg ...

Page 45: ... in the single liquid delivery mode FLOW mode NOTICE Once this setting is made it is memorized and need not be made each time POWER ON SETUP UTILITY When pressing the POWER and SETUP UTILITY keys simultaneously the pump mode setting screen appears It may be necessary to continue pressing SETUP UTILITY until the setting screen appears ENTER 1 ENTER 1 ENTER SETUP PUMP MODE CONTRAST S NO A LOW 4 02AB...

Page 46: ...dient mode V HIGH High pressure gradient with solenoid valves mode FLOW Isocratic mode POWER OFF Turn the power off once POWER ON Turn the power on again GR MODE LOW HIGH V HIGH 4 FLOW 4 SETUP PUMP MODE CONTRAST S NO A FLOW 4 02AB 111 TIME FLOW PRESS 0 0 1 000 0 0 1 ...

Page 47: ...rate is 1 0 mL min PROGRAM No 1 PRESSURE VALUE MAX MIN MPa 20 0 0 0 TIME FLOW EVENT min ml min 1 2 3 4 0 0 1 000 12 22 32 42 Monitor screen If manual setting screen is displayed press INITIAL to obtain the programming mode PROG Program generation is started SET PROGRAM No 1 9 1 Input program No Valid program numbers are from 1 to 9 MODIFY 0 NEW 1 0 Generates a new program or modifies an existing p...

Page 48: ... Input an event to be executed within the entered time TIME FLOW EVENT 1 A new input screen will appear so enter time flow rate and event ESCAPE Monitor screen NOTICE A maximum of 99 steps can be entered for program Nos 1 to 9 NOTICE The entered program is saved When power is turned on next time the initial screen TIME 0 of the program used just before power was turned off will appear ...

Page 49: ...pressure limit at 15 0 MPa and lower pressure limit at 0 MPa Monitor screen MANUAL MANUAL FLOW 1 PRESS 2 MN 1 Select the constant flow rate mode CONSTANT FLOW FLOW MAX MIN MN 1 000 15 0 0 0 Input the flow rate and the upper and lower limit pressure values CONSTANT FLOW FLOW PRESS MN 0 000 0 0 The monitor screen will apper Monitor screen NOTICE The set conditions for manual operation will be erased...

Page 50: ...ssure mode CONSTANT PRESS INITIAL FLOW PRESS MN 1 000 15 0 Input the flow rate and the pressure values CONSTANT FLOW FLOW PRESS MN 0 000 0 0 The monitor screen will apper Monitor screen Before stating the pump check that the pressure level is 0 Mpa NOTICE The liquid delivery will not start unless the starting pressure is 0 MPa NOTICE The set conditions for manual operation will be erased when powe...

Page 51: ... organic solvents having an ignition point below 70 C When using flammable chemicals be careful about possible ignition due to static electricity Particularly when using non conductive chemicals employ a conductive vessel made of metal or the like and provide grounding connection correctly WARNING Explosion of Vapor from Flammable Chemicals If a flammable chemical such as organic solvent leaks fro...

Page 52: ...p the pump column and detector and wait until the baseline stabilizes 5 Inject a sample 6 When analysis is finished turn the power off and dispose of the waste solution NOTICE Before connecting a new column make sure the flow path is filled with mobile phase so that air won t enter the column NOTICE The whirring sound of the pump during operation varies depending on such operating conditions as fl...

Page 53: ...ROGRAM enter the upper limit pressure level lower limit pressure level mixing ratio flow rate and event PROG No 1 PRESSURE MAIN MAX MIN MPa 20 0 0 a TIME FLOW EVENT 0 0 1 000 10 0 1 000 1121 31 41 10 1 1 000 20 0 1 000 b 25 0 10 20 30 40 30 0 2 000 30 1 1 000 40 0 1 000 00 92 Fig 3 4 Example of Program a For EVENT up to 4 event codes are settable at each time point It is possible to generate conta...

Page 54: ...kip all the entries you do not want until you reach event column In the SET PROGRAM mode the interpolation of mixing ratio flow rate etc that occurs in a time program is as follows Parameter Flow Rate Interpolation Linear TN TN 1 TN TN 1 Preset time NOTICE For generating a stepwise gradient program utilize TN 1 TN 0 1 3 9 ...

Page 55: ... NEW 1 1 ENTER MAX ENTER MIN ENTER Upper limit pressure level Lower limit pressure level TIME Set time Ti ENTER FLOW Set flow rate Fi ENTER EVENT Event code E1i ENTER Event code E2i ENTER Event code E3i ENTER Event code E4i ENTER 8 Repeat steps 5 to 7 until you define all programs lines 9 To terminate the program generation press ESC 1 2 3 4 5 6 7 7 7 7 ...

Page 56: ...w rate valid entries are from 0 00 to 2 499 L 2100 Flow rate valid entries are from 0 00 to 9 999 L 2130 7 Input the event code At the set time point you can stop the pump generate an audible signal markers timers etc When properly interfaced these signals can be output to an external system No display will show on the screen unless an event is set 8 Repeat steps 5 to 7 until you complete the prog...

Page 57: ...m ENTER MODIFY 0 NEW 1 1 ENTER ESCAPE 3 Deletion of Step How to delete one step in a program is shown next SET PROG SET PROG No ENTER MODIFY 0 NEW 1 No of program containing step to be deleted m 0 ENTER MAX ENTER MIN Upper limit pressure level ENTER DELETE ENTER ESCAPE Lower limit pressure level 1 2 3 1 2 3 4 5 6 7 ...

Page 58: ...pressure rises beyond the level set here the pump automatically stops 4 If pump pressure falls below the level set here the pump automatically stops 5 Press the key until the step you want to delete appears on the screen If display advances beyond that step press the key 6 Press the DEL key when the cursor is on the TIME line want to delete 7 To finish editing press the ESC key The display should ...

Page 59: ...step m MODIFY 0 NEW 1 0 ENTER MAX ENTER MIN ENTER Upper limit pressure level Lower limit pressure level TIME Time point of step added Ti ENTER FLOW Set flow rate Fi ENTER EVENT Event code E1i ENTER Event code E2i ENTER Event code E3i ENTER Event code E4i ENTER 1 2 3 4 5 6 7 7 7 7 ...

Page 60: ... pump automatically stops The 5 Input the time point of the step you want to add 6 Flow rate valid entries are from 0 00 to 2 499 L 2100 Flow rate valid entries are from 0 00 to 9 999 L 2130 7 Input the event code At the set time point you can stop the pump generate an audible signal markers times etc When properly interfaced these signals can be output to an external system No display will show o...

Page 61: ... containing step to be deleted m MODIFY 0 NEW 1 0 ENTER MAX ENTER MIN ENTER Upper limit pressure level Lower limit pressure level TIME Time point of step added Ti ENTER FLOW Set flow rate Fi ENTER EVENT Event code E1i ENTER Event code E2i ENTER Event code E3i ENTER Event code E4i ENTER 2 3 4 5 7 8 1 8 8 8 6 ...

Page 62: ...ically stops 5 Press the key until the step to be corrected appears on the screen If the display advances beyond that step press the key 6 When a correction is unnecessary press the ENTER key only The allowable input range is 0 00 to 2 499 7 When a correction is unnecessary press the ENTER key Valid flow is from 0 00 to 2 499 L 2100 Valid flow is from 0 00 to 9 999 L 2130 8 Enter the event code Wh...

Page 63: ...ter deletion method is shown next SET PROG SET PROG No ENTER No of program containing parameter to be deleted m MODIFY 0 NEW 1 0 ENTER MAX ENTER MIN ENTER Upper limit pressure level Lower limit pressure level ENTER DELETE ENTER 2 3 4 5 1 6 7 ...

Page 64: ... the pump automatically stops 4 If pump pressure falls below the level set here the pump automatically stops 5 Press the key until the step to be corrected appears on the screen If display advances beyond that step press the key 6 Move the cursor to the parameter to be deleted 7 To delete the parameter press the DELETE key and then press ENTER key ...

Page 65: ... the pressure sensor etc Check the pressure zero point once a month If it has drifted then stop the pump open the drain valve and carry out the following operation Monitor screen UTLTY UTILITY CONDITION 1 UNIT 2 FLUSH 1 EVENT 4 LINK OTHERS 6 Select CONDITION CONDITION SET Z ADJ 1 MOTOR CTRL 2 1 PURGE 3 CALIBRATION 4 Select Z ADJ PRESS ZERO ADJUST PRESS ZERO ADJUST YES 1 NO 0 OPEN DRAIN VALVE 0 Ope...

Page 66: ...CONDITION 1 UNIT 2 FLUSH EVENT 4 LINK OTHERS 6 Select CONDITION CONDITION SET Z ADJ 1 MOTOR CTRL 2 2 PURGE 3 CALIBRATION 4 Select MOTOR CTRL MOTOR CONTROL MOTOR CONTROL STANDARD 1 FIX 2 1 STANDARD Monitor screen MOTOR CONTROL is set to STANDARD FIX FIX STEP AREA 0 30 300 100 Enter a FIX STEP AREA Input range is 0 and from 30 to 300 Monitor screen NOTICE Select STANDARD for Model L 2100 2130 pump ...

Page 67: ...OR CTRL 2 2 PURGE 3 CALIBRATION 4 Select PURGE PURGE FLOW FLOW 0 001 2 499 2 499 Enter a purge flow rate Monitor screen Pressing the Purge key in PUMP ON status activates purging at the set flow rate Pressing the Purge key again during purge will reset the purge function During purge the upper pressure limit is set at 2 9 MPa 30 kgf cm2 and the lower pressure limit at 0 MPa 0 kgf cm2 ...

Page 68: ...en UTLTY UTILITY CONDITION 1 UNIT 2 FLUSH 1 EVENT 4 LINK OTHERS 6 Select CONDITION CONDITION SET Z ADJ 1 MOTOR CTRL 2 4 PURGE 3 CALIBRATION 4 Select CALIBRATION CALIBRATION FLOW 1 PRESS 2 Select FLOW FLOW CALIB 0 97 1 03 1 000 Enter a parameter o flow rate calibration Monitor screen ...

Page 69: ...n UTLTY UTILITY CONDITION 1 UNIT 2 FLUSH EVENT 4 LINK 5 OTHERS 6 Select CONDITION CONDITION SET Z ADJ 1 MOTOR CTRL 2 4 PURGE 3 CALIBRATION 4 Select CALIBRATION CALIBRATION FLOW 1 PRESS 2 2 Select PRESS FLOW CALIB 0 900 1 100 1 000 Enter a parameter of pressure calibration Monitor screen ...

Page 70: ...VENT 4 LINK OTHERS 6 Select UNIT PRESS UNIT bar 1 psi 2 kgf cm2 3 1 4 1 MPa 4 Select pressure unit Monitor screen Pressure indication unit is set to selected one The set pressure unit is retained in memory even after turning off power supply The relationship among selectable pressure units is listed below bar psi kgf cm2 MPa 10 0 145 10 2 1 00 ...

Page 71: ...the salt component can be prevented by flushing the column with water An example is given where the column is flushed for 30 minutes with 100 liquid C 15 minutes after the end of analysis Monitor screen UTLTY UTILITY CONDITION 1 UNIT 2 FLUSH 4 EVENT 4 LINK OTHERS 6 Select FLUSH PUMP AUTO STOP YES 1 NO 0 1 Select YES PUMP AUTO STOP COLUMN FLUSH YES 1 NO 0 0 YES SET DELAY TIME TIME min 1 0 600 0 600...

Page 72: ...HING TIME TIME min 1 0 600 0 30 0 Column flushing time can be input within 1 0 to 600 0 min Monitor screen NO Monitor screen When the time for column flushing arrives the flushing is executed according to No 9 program NOTICE The program for column flushing is fixed at No 9 Upon selecting column flushing program No 9 is automatically set for flushing When column flushing is set program No 9 is not ...

Page 73: ...specified with autosampler etc Stabilizing time is the time from the end of one analysis until the start of the next analysis The pump stops when delay time has ended 2 With AUTO STOP at NO n indicates number of analyses specified with autosampler etc The pump remains in liquid delivery status Analysis 1 Analysis 2 Analysis 3 Analysis n Delay time Stabilizing time Pump stop Delay time Column flush...

Page 74: ...sure limiter error occurs a signal can be output at event code No 4 Monitor screen UTLTY UTILITY CONDITION 1 UNIT 2 FLUSH 4 EVENT 4 LINK OTHERS 6 Select EVENT EVENT SET PRESS LIMIT 1 EVENT CODE 2 1 PRESS LIMIT PRESS LIMIT NONE 0 PULSE 1 LEVEL 2 0 2 1 EVENT NO 4 Enter the desired code If a pressure limit error is detected an event signal is output at EVENT No 4 Monitor screen SET EVENT CODE 0 92 In...

Page 75: ...rks 1 2 3 4 10 20 30 40 11 21 31 41 12 22 32 42 1st digit Timer no 2nd digit Action pattern Timer Action Contact closes for 1 sec Pump 00 Pump stops Buzzer 92 Buzzer sounds for 5 sec Start point Contact opens Contact closes Signal turns off Close marker Open Signal turns on Start point 1 s ...

Page 76: ...EVENT 4 LINK OTHERS 6 Select OTHERS OTHERS ERROR IN 1 LEAK SENSOR 2 1 Select ERROR IN ERROR IN SIGNAL DISABLE 0 PUMP OFF 1 1 DISABLE The pump is not used ERROR signal to stop the unit in e Line PUMP OFF The pump stops the flow when it received the ERROR signal in e Line Monitor screen ...

Page 77: ...een UTLTY UTILITY CONDITION 1 UNIT 2 FLUSH 6 EVENT 4 LINK OTHERS 6 Select OTHERS OTHERS ERROR IN 1 LEAK SENSOR 2 2 Select LEAK SENSOR LEAK SENSOR OFF 0 ON 1 0 Select ON for using the leak sensor or OFF for avoiding its use Monitor screen ...

Page 78: ...ayed in the following procedure Monitor screen GLP GLP LOGBOOK 1 PRESS M 2 1 KEY LOCK 3 Select LOGBOOK LOG BOOK REPORT 1 RESET 2 1 Select REPORT for displaying logbook or RESET for resetting the logbook LOGBOOK REPORT SOLVENT 99999 1 2 25 2002 Total flow after replacement of the pump seal will be displayed Pump seal replacement date month day year is indicated in parentheses ESC Monitor screen REO...

Page 79: ... month M day D and year Y Upon resetting total flow value is reduced to 0 and the integration of flow starts Unless the ENTER key is pressed after entry of all date values resetting does not become effective Even when the year is the same as the displayed one you should enter all values and then press the ENTER key Monitor screen RESET ...

Page 80: ...or a pressure drop This may be caused by formation of air bubbles in the flow path Monitor screen GLP GLP LOGBOOK 1 PRESSURE M 2 2 KEY LOCK Select PRESSURE M PRESSURE MONITOR 9999 TIMES Display the number of double speed section errors ESC Monitor screen Resetting occurs on the following occasions On input of PUMP ON or PUMP OFF On activation of contact On start of gradient elution On reception of...

Page 81: ... GLP GLP LOGBOOK 1 PRESSURE M 2 3 KEY LOCK 3 Select KEY LOCK KEY LOCK YES 1 NO 0 1 Locks the keys on the operation panel in order to prevent undesired key entries Monitor screen LOCK OFF ESC Key lock can be released by the ESCAPE LOCK OFF key Monitor screen 3 8 3 ...

Page 82: ...ergent sufficiently of connect it through a degasser 2 Items for Performance Check Prepare the following items for performance check Load resistance coil A coiled stainless tube with an inner diameter of 0 25 mm and a length of 10 m 60 methanol in about 1000 mL A mixture of methanol 60 and distilled water 40 in volume This should be prepared separately Distilled water in about 500 mL 3 Performance...

Page 83: ...ctor and pump 3 Check if the drain tube is properly connected to the waste liquid bottle The waste liquid bottle must be at a lower position than the autosampler 5 1 2 Preparing the System for Operation Detector wavelength 250 nm time constant 0 1 s Autosampler Pump Load resistance coil stainless pipe 0 1 mm i d 10 m long L 2100 stainless pipe 0 25 mm i d 10 m long L 2130 Eluent Detergent Injectio...

Page 84: ...ove steps connect the eluent to the pump and the detergent to the autosampler Be sure to use the eluent and detergent of the same production lot 7 Start the pump to fill each flow path in the pump and autosampler with liquid 8 After the eluent has reached the injection valve of autosampler turn on the autosampler power supply If the injection valve is switched over when no liquid is INITIAL the va...

Page 85: ...epare 60 methanol for the eluent and detergent Wait until the pump detector etc are warmed up and the baseline stabilizes 3 Place a vial containing at least 1 mL of standard sample in rack position No 1 and set the rack in the autosampler 5 Press the START key of autosampler to start analysis The analysis is carried out 6 times The operating conditions during analysis are indicated on the display ...

Page 86: ...ake sure the RSD value is 0 075 or less However this value represents the reproducibility of not the pump alone but the entire chromatographic system Therefore the value varies with each stability of the autosampler and detector accuracy of the data processing unit and its processing parameters ...

Page 87: ...t stop the pump just after completion of an analysis The pump must be kept running for 30 minutes after completion of an analysis 2 Do not introduce a sample containing particles Ensure that the sample contains no particles or impurities 3 Periodically clean the column Components left in the column will contaminate it Part of a complex sample is apt to remain in the column This not only causes poo...

Page 88: ...ept clean Clean the pump every month For cleaning just pass isopropanol through the pump for 30 minutes 3 The high pressure pump does not function properly when there are bubbles Before exchanging the solvent bottle or adding solvent into the bottle be sure to turn off the pump Deterioration of the seal causes leaks Leaks lead to poor baseline stability and data reproducibility The net result of l...

Page 89: ...eware of Electric Shock Before removing the pump cover for parts replacement etc make sure to turn OFF the power switch and disconnect the power cord from the receptacle 1 If an abnormality occurs on the Model L 2130 pump an error message appears on the LCD Liquid Crystal Display Error displays result from either the detection of a fault during the self diagnostics at power up upon INITIAL ializat...

Page 90: ...M ERROR RAM ERROR EPROM ERROR MOTOR INITIAL IALIZE ERROR E LINE ERROR Error generating condition Power ON Pump operation PRESSURE MAX LIMIT ERROR PRESSURE MIN LIMIT ERROR EXTERNAL STOP Error generating condition Fig 5 1 Error Generation and Error Messages YES YES NO NO ...

Page 91: ...y the service personnel 3 RAM ERROR The result of RAM check is abnormal Turn the power off and then on again If the same error occurs notify the service personnel 4 EPROM ERROR Contents of EPROM are abnormal Turn the power off and then on again If the same error occurs notify the service personnel 5 PRESSURE MAX LIMIT ERROR Upper pressure limit exceeded 1 Check the flow for leaks or clogging 6 PRE...

Page 92: ...ESCAPE key 2 Set the programming mode by pressing the INITIAL key 4 Purging cannot be set or canceled by pressing the PURGE key Display is other than the monitor screen Numerical input is awaited Call the monitor screen by pressing the ESCAPE key 5 After appearance of the Initialization screen when the power switch has been turned on the E Line connection wait screen remains on the screen The disp...

Page 93: ...thanol at a rate of 1 0 mL min and pressure of 19 6 MPa Part No Name Shape and Specifications Position Used Normal Life 890 1130 Seal for L 2100 Pump head 640h 655 1080 Seal for L 2130 Pump head 640h 885 2920 Seal Drain valve 1920h 890 1128 Plunger for L 2100 Pump head 890 1125 Plunger for L 2130 Pump head 890 1206 Check valve on discharge valve for L 2100 890 1205 Check valve on suction valve for...

Page 94: ...w Path Tubing Parts Part No Name Shape and Specifications Position Used L329235 SUS push screw M6 1 16 inch 1 57 mm OD SUS pipe 655 1458 SUS push screw S 1 16 inch 1 57 mm OD SUS pipe Rheodyne injector 655 1459 SUS push screw M 1 16 inch 1 57 mm OD SUS pipe Rheodyne injector 11 M6 9 6 No 10 32 UNF 13 5 No 10 32 UNF Filter 638 1423 Packing 638 1422 Packing B 810 1344 Suction valve Discharge valve 2...

Page 95: ...mm OD PTFE tube 810 1312 PCTFE push screw 2 1 ID 2 mm OD PTFE tube 638 1099 SUS ferrule SUS 316 1 16 inch 1 16 inch OD SUS pipe L369053 PCTFE ferrule 1 16 inch 1 16 inch PTFE tube F275108 PTFE tube 0 25 mm ID 1 57 mm OD 10 m Inlet tube for UV detector 13 5 M8 14 M6 10 5 No 10 32 UNF 3 9 3 9 ...

Page 96: ... mm OD 10 m Connecting tube between proportioning valve and pump 638 1140 PTFE tube 1 5 mm ID 3 0 mm OD 10 m Solvent suction tube for pump 634 1976 Stainless steel pipe 0 25 mm ID 1 57 mm OD 5 m Pipe for high pressure gradient elution Pipe for between injector and column 634 1977 Stainless steel pipe 0 5 mm ID 1 57 mm OD 10 m Inlet outlet pipe for dynamic mixer 638 2185 Stainless steel pipe 0 8 mm...

Page 97: ...6 5 cont d Part No Name Shape and Specifications Position Used 885 1601 Nipple made of SUS Same as above 885 2179 Nipple made of Daiflon Same as above 24 No 10 32 UNF M6 21 5 No 10 32 UNF No 10 32 UNF ...

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Hitachi L-2100, Instruction Manual

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Summary of Contents for L-2100

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