GeminiBio ORFLO Moxi V User Manual Download Page 37

Moxi V

 User Guide

 

 

Page 33 

 

Size Gating of Scatter (Dot) Plots 

The results of all fluorescent tests (all tests except for the “Size Histogram” test) are initially 
displayed as scatter plots with the size (blue) gates enabled (see image above, left).  Gates can 
be adjusted by touching and dragging the blue markers around the edges of the scatter plot 
(above).  Touching the marker at the base of the left gate toggles the marker from green 
(unlocked, images above/left and above/middle) to red (locked, image above/right).  Touching 
the marker again will switch it back to the prior state.  Moving the left cursor with base unlocked 
(green) results in the base moving synchronously/equivalently with the top left cursor (gate line 
is forced to be vertical).  Moving the left cursor with the base marker locked (red) anchors the 
base point allowing the user to pivot the top point around it (e.g. image above/right).  This top 

Summary of Contents for ORFLO Moxi V

Page 1: ...For Research Use Only Not For Use In Diagnostic or Therapeutic Procedures a subsidiary of USER GUIDE...

Page 2: ...ware in violation of any applicable laws orders or regulations Disclaimer The information in this document is subject to change without notice and should not be construed as a commitment by Orflo Neit...

Page 3: ...RUNNING A TEST 12 Instructions Cell Count Size Viability PBMC Check CAR T Expansion Tests 12 Inserting a Cassette 12 System Calibration 12 Loading a sample 13 Cell QC Test 14 Cell QC Single Run 15 Cel...

Page 4: ...ws 39 FILE TEST OPERATIONS 41 Test Naming and Renaming Tests 41 Saving and Deleting Data Tests 41 Exporting Data Screenshots 42 Editing Saved Tests 42 Compare Overlay Saved Tests 44 EXPORTING DATA CON...

Page 5: ...tended for research use only Not for use in diagnostic procedures It is not intended for use in medical diagnostic or therapeutic procedures and it has not been approved or cleared by the United State...

Page 6: ...ICATED THROUGHOUT THE USER GUIDE BEFORE OPERATING THE SYSTEM Electrical Safety WARNING To avoid the danger of electric shock including the possibility of permanent injury or death Prior to use verify...

Page 7: ...mble or service the Moxi V to service or adjust the laser The instrument has no user serviceable parts and attempts to adjust or service the laser may result in hazardous radiation exposure All servic...

Page 8: ...ter one pack of 25 Type S MXC030 Cassettes a spare cassette tray insert and a Quick Start Reference Guide The following figure shows the main visible components of the Moxi V and the table provides a...

Page 9: ...each end thereby allowing for two tests to be run per cassette Moxi VTM Accessories The Moxi V uses a USB Cable and Power Adapter for power source The Moxi V Kit will come with the power adapter requ...

Page 10: ...V is used with Type S or MF M Cassettes Refer to the Specifications section for information on the operating size and concentration ranges for the system The sample volume for a test should be 60 L Ce...

Page 11: ...ditional Materials Required Cell or bead sample diluted and dissociated if necessary 60 L minimum Type S MXC030 recommended for almost all assay or MF M MXC010 Cassettes Pipette typically P100 or P200...

Page 12: ...he Home Screen Home Start Screen NOTE The home page appearance may change as additional applications are launched and new GUIs graphical user interface buttons are added to the Moxi V software program...

Page 13: ...ain menu of the instrument Then touch the Date or Time field Use the up down arrow keys to change the values Passcode Lock The Moxi V can be setup to require a password login to use the instrument and...

Page 14: ...to the top of the scatter plot and compressing or piling up there then the fluorescent gain should be lowered Alternatively if the fluorescence signal is compressed towards the bottom of the plot a hi...

Page 15: ...ging of cassettes Clusters aggregates can be broken apart with mechanical trituration and or protease dissociation e g Accutase or Accumax Larger particles or stubbornly aggregated clusters can be rem...

Page 16: ...ay and slide a test cassette with ORFLO lettering side up into the cassette tray either end 1 or 2 Close the Door Note If the cassette had been previously used the system will generate a Used or Inval...

Page 17: ...f the cassette either test 1 or test 2 depending on which end of the cassette was inserted into the instrument and close the Door Note Open the door only to the first stop first point of resistance wh...

Page 18: ...es to Counting and the results are dynamically populated on the scatter dot plot Results for most samples will be complete in approximately 10 seconds but may be longer for higher concentration sticky...

Page 19: ...an be changed by touching in the box and using the keyboard below to type a new name Notes 1 Capital letters can be typed by hitting the CAPS key to change the keyboard to capital letters 2 Additional...

Page 20: ...older with the naming format of QC XXX fcs where XXX is a sequential number starting at 000 and incrementing with each test run in the batch The Sample ID field can be used as general text description...

Page 21: ...to identify the core cell population by size and determine the viability by PI fluorescence The user is provided the opportunity to review the system gate placement by touching the Back button Touchin...

Page 22: ...ing more samples as described below in the Cell QC Test Output Continuing Batch Testing section Cell QC Test Output Continuing Batch Testing Touching the Save button for a Batch Run will save the late...

Page 23: ...screenshot files and the batch summary file Batch_summary csv file An example screenshot of a Batch_summary csv file opened in Microsoft Excel in this case is shown above The summary file contains the...

Page 24: ...the keyboard to capital letters 2 Additional punctuation symbols are available by touching Punc Touching enter will start the test beginning with the laser cassette alignment zone see laser cassette a...

Page 25: ...PIN Diode fluorescence Values for each event are binned across a 1000 point histogram and displayed 2 Fluorescence vs Size scatter plots all fluorescent assays For each event the recorded PIN diode m...

Page 26: ...Histogram overlay toggle Upper gated region Above red line statistics results Next button to progress through screen workflow Store recall gate locations in memory pre sets Select axis Channel displa...

Page 27: ...red Line defined in PMT vs Size view pop results Dead Cells Lower Upper overlap Region yellow Fluorescence scale PMT output in mV with logicle scaling Lower gated red text shows below red line compen...

Page 28: ...particle diameter Note This is an effective diameter that is based on a precise volumetric measurement Coulter Principle combined with a spherical assumption particle volume Coulter Principle measured...

Page 29: ...luded in the histogram binning Simplified Output and Auto Gating Overview The two main outputs listed above are the main way raw data is viewed and analyzed Ultimately the data needs to be gated to pr...

Page 30: ...the degree of red blood cell RBC contamination red box image above This value is calculated as the cell counts in the range of 5 m to 6 2 m corresponding to the region where RBC s would be expected Th...

Page 31: ...itions dashed red arrows image above right are displayed The range of the RBC gate can be adjusted by touching and dragging the blue icon more detail in the Manual Gating Fluorescence vs Size Gating s...

Page 32: ...is display above middle The placement of the size gates blue markers vertical lines two top dashed red arrows in image below right and fluorescence gate horizontal red line two lower dashed red arrows...

Page 33: ...y that gate for the initial test run with any new sample cell types Once verified the user can simply refer to the simplified test output screen The user can return to the simplified output summary sc...

Page 34: ...ts are presented to the user immediately following a test in the form of a scatter dot plot below left for fluorescent tests or as a size only histogram below right Size Histogram test only Note Detai...

Page 35: ...This is accomplished by selecting the re scale button below the scatter plot magnifying glass with arrow see image sequence for a Moxi V Type S cassette below With each button press the text under th...

Page 36: ...des by touching the red icon in the upper right of the scatter plot see images below Each time the red gate icon is touched the system toggles through the gate modes enabling a different set of adjust...

Page 37: ...Touching the marker at the base of the left gate toggles the marker from green unlocked images above left and above middle to red locked image above right Touching the marker again will switch it bac...

Page 38: ...uorescent Gating of Scatter Dot Plots Touching the red gate toggle marker when the size blue markers are currently enabled correspondingly disables the blue size gate markers and enables the red fluor...

Page 39: ...ine the points that will be identified as noise These noise points will be grayed out image below middle and will not be saved with the test Note DATA GATED AS NOISE WILL NOT BE SAVED WITH YOUR FILE A...

Page 40: ...image above right Selecting one of the buttons will automatically apply the gate parameters that were saved at that current location A full gating sequence is illustrated in the image above In the abo...

Page 41: ...ased histogram is displayed as opposed to the traditional fluorescence vs size scatter dot plot Upon completion of the test counts for the Size histogram can be obtained by moving the blue gating mark...

Page 42: ...evitably present To help identify this noise the counts in this region are automatically grayed out by the system In size only mode an MPI value is also provided in the yellow box at the top right of...

Page 43: ...splay axis the X Y button image below frame at the top right can be selected When X Y is pressed the available channels for each axis are displayed in the gray boxes at the top and to the right of the...

Page 44: ...rescent gate defined in the corresponding fluorescence vs size histogram image above frame in the top middle for this example Pin Diode 561nm LP fluorescence vs size scatter plot image above bottom le...

Page 45: ...istogram see image below left The default name is presented and can be deleted by hitting the Clear key or successive Backspace keys Capital letters can be achieved by hitting the Caps button Symbols...

Page 46: ...bmp extension is used so that it does not overwrite any screenshot of the simplified view The screenshot will be an exact pixel by pixel representation of the current screen display without the butto...

Page 47: ...cending vs descending Test files and screenshot files can be opened by simply touching the green or dark gray row An example is shown in the image above frames from top middle to bottom left on touchi...

Page 48: ...ton will turn from black to green indicating the compare option is viable The criteria for comparing two tests is as follows The tests need to be of the same assay type e g Viability PBMC etc Only two...

Page 49: ...Moxi V User Guide Page 45...

Page 50: ...for each test and the files are named with the test name followed by a fcs extension e g Via 001 fcs Quantitative fluorescence values fluorescent assays and size values all tests are saved within the...

Page 51: ...rer as a flash drive e g E If the Moxi V is not physically connected to the computer you will receive a message USB Connection Please connect a USB cable to a PC host first i Once connected the comput...

Page 52: ...he lost connection The Connected light will go dark images above left to above middle iii Touch the I have Ejected or Safe Disconnected at the host PC button The system will return to the Home Screen...

Page 53: ...S being run on the unit the build date as well as the Bluetooth ID number see image to the right This information is required by ORFLO Technical Support when assisting users with troubleshooting issue...

Page 54: ...ssing when the text appears The loader mode is shown in the image to the right top 5 The Moxi V will appear as an external drive on the computer look for it in the Windows Explorer as External Disk Dr...

Page 55: ...Moxi V User Guide Page 51...

Page 56: ...rt that has the adhesive build up replacing it with a new insert or an old one that has had the adhesive cleaned off Ordering Information Complimentary replacement inserts up to 5 per instrument per y...

Page 57: ...elow Installing an insert IMPORTANT Make sure to unplug Moxi V USB Power cable from system before starting this procedure Leaving it in place can result in damage to both the cable and the system when...

Page 58: ...Moxi V User Guide Page 54 3 Push the cassette insert into the system Tray Guides Insert Rails...

Page 59: ...instrument pump Contact ORFLO Technical Support Instrument stops responding Internal firmware issue due to instrument malfunction or high level of external interference Reset instrument by pressing a...

Page 60: ...diluent Cells should be suspended in 0 9 salt solution e g PBS or equivalent to ensure proper conductivity for unit operation and to ensure proper particle sizing Water hypotonic or hypertonic solutio...

Page 61: ...adapter NOT A COMPUTER USB PORT and re try Make sure the battery is fully charged If the battery is not fully charged keep the system plugged into the wall outlet make sure it is powered on and charge...

Page 62: ...other side of the cassette or a new cassette Used or Invalid cassette Previously used sensor detected Do not reuse sensors User System Defaults loaded New Firmware has been loaded No problem simply t...

Page 63: ...Cassettes at room temperature in a dry environment For best results cassettes should be used within one year of purchase Avoid prolonged exposure to ultraviolet light as it may degrade the touch scre...

Page 64: ...lugged the level of fill of the battery icon will be proportional to level of charge of the battery e g a 50 charged system will show a half filled battery icon when unplugged The battery may be charg...

Page 65: ...ine maintenance required for the Moxi VTM In addition there are no user serviceable parts with the exception of the ability to swap out the tray insert see instructions above Instrument repairs must b...

Page 66: ...cassette Sample Volume Required Particle Cell Size Range Type S cassette MF M cassette Concentration range Type S cassette Counts Optimal fluorescence MF M cassette Counts Optimal fluorescence Measur...

Page 67: ...when handled stored properly no cigarette smoke no exposure to extreme temperature cycles general storage temperature of 15 30C The cassettes can be handled touched anywhere on the cassette with the...

Page 68: ...e found on our website at www orflo com How Long is Your Warranty Your ORFLO products are covered for a period of one 1 year from the original date of purchase from ORFLO or an approved sales distribu...

Page 69: ...ss or Implied Warranties and Other Legal Stuff ORFLO Technologies makes no other warranty express or implied There is no warranty or merchantability or fitness for a particular purpose The warranty pr...

Page 70: ...Moxi V User Guide Page 66 A subsidiary of...

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