Moxi V
™
User Guide
Page 6
Getting Started
The Moxi V™ is shipped in a condition ready for initial use; though we recommend checking for
software updates and updating the Moxi V with the most current software version upon receipt.
To begin, plug the USB Cable into the instrument at one end (mini-USB). Plug the opposite end
of the USB Cable (standard USB) into the Power Adapter. Plug the Power Adapter into an AC
outlet that matches the rating of the Power Adapter.
CAUTION
Use only an ORFLO supplied USB Cable and Power Adapter. Use of
other products may result in inaccurate test results or damage to the
instrument, and will void warranty.
NOTES
Tests should only be performed with the instrument powered by an AC
source.
The device will power on and users can access files without attaching to a
power outlet but for data collection the device must be plugged into an
electrical outlet.
General Guidelines
The Moxi V is used with Type S+ or MF-M Cassettes. Refer to the Specifications section for
information on the operating size and concentration ranges for the system.
The sample volume for a test should be 60µL.
Cells should be suspended in 0.9% salt solution (e.g. PBS or equivalent) to ensure proper
conductivity for unit operation and to ensure proper particle sizing. Water, hypotonic, or
hypertonic solutions are
not
acceptable diluents.
Cells need to be prepared as single-cell suspensions. Clusters/aggregates should be broken
apart with mechanical trituration (e.g. pipetting vigorously) and/or protease dissociation (e.g.,
Accutase
®
or Accumax
®
). For stubbornly aggregated samples, pre-filtration with a 40µm cell
strainer is recommended.
As the Moxi V™ uses a 532nm laser with a 561nm/LP filtered PIN diode for detection.
Dye/fluorophore selection should be based accordingly. As the system is intended as a cell
viability system, the main recommended fluophore is Propidium Iodide (PI, recommended
2µg/ml final PI concentration). Please refer to the
Orflo Viability Staining Protocol
for the
staining protocol for viability assessments.
