Moxi V
™
User Guide
Page 32
Rescaling the axis results in reprocessing of the raw data to ensure that resolution is not lost
during the rescale process (1000 histogram bins). This allows (and is recommended for) users
to achieve finer resolution of smaller particle populations and potentially increased separation
between particle sub-populations.
However, once a test has been saved, rescaling the x
axis is not a possibility.
Note: Any events that are off-scale (i.e. larger than the maximum
displayed scale) are not included in the statistics.
Gating the data
Overview
Immediately following a test acquisition (excluding the “Size Only” test assay, which is described
below), there are three gating modes/categories: 1.) Noise Gating, 2.) Size Gating and 3.)
Fluorescence gating. The user can cycle through each of the modes by touching the red icon in
the upper right of the scatter plot (see images below). Each time the red gate icon is touched,
the system toggles through the gate modes, enabling a different set of adjustment markers.
The yellow gate/dot allows the user to adjust the noise gate location to define the range of data
to be saved (or more specifically, to demarcate data to be excluded from being saved). The
blue dots allow the user to identify the size range that will be used for generating the summary
information (statistics) on the sample. The red dots allow the user to adjust the fluorescent
threshold to determine which events will be included in the positive vs. negative designations for
the display fluorescence channel. Each gating mode will be discussed in further detail in the
corresponding sections below.
Please note that using “histogram overlays” of the scatter
plot can greatly facilitate the placement of the gates (refer to section on “Histogram
Overlays on Fluroescence vs. Size Scatter Plots”).
