Moxi V
™
User Guide
Page 26
“PBMC Check" Output
The initial, simplified output of the PMBC test is shown above. This output is based on the
system’s auto-gating of the PIN Diode fluorescence (Propidium Iodide, Viability) vs. Size scatter
plot (more detail below) to identify the core PBMC population (by size) and determine the
viability (by PI fluorescence). The core information is provided at the top with total PBMC count
(e.g. 243,451 cells/ml in the image above) and Viability (e.g. 51%, image above). Further detail
on the statistics (concentration, cell diameter, cell volume, and median fluorescence intensity)
are provided below for both viable (green box, image above) and dead cell (orange box, image
above) populations. Finally, the system calculates the degree of red blood cell (RBC)
contamination (red box, image above). This value is calculated as the cell counts in the range
of 5µm to 6.2µm, corresponding to the region where RBC’s would be expected. The user has
the option to adjust that gate/region depending on the profile of the RBC contamination is
presented as the (total RBC counts / total PBMC counts) * 100%. As the RBC contamination
are presented as a ratio/percentage relative to the PBMC counts, the number can exceed
100%.
The data scatter plots/histograms and associated system-set gates can be viewed and adjusted
by the user. It is recommended, particularly for the first test in a sequence of experiments, that
these gates be checked by the user. The scatter plot is accessed by touching the “Next” icon
(image below/right).
The user can save, print a screenshot (bitmap), or delete the test by using the “File button at the
bottom left. Touching the button brings up the three options.
