Moxi V
™
User Guide
Page 16
Cell QC – Start Batch
Selecting the “Start Batch” file option will prompt a warning that this mode is only meant for
samples of the same cell type (see image above/middle). The reason for this is that, following
the first test (which will be auto-gated by the system, with the option to manually adjust the
gates), the subsequent tests will be run with the same gate settings as the first. This is
intentionally implemented for consistency in running sample batches. With batch testing, there
are three test parameters that the user can set: 1.) “Batch Name” 2.) “Sample ID” and 3.)
“Dilution Factor”. The system defaults the batch name to have the format of “QC-YYYYMMDD-
HHMMSS” (where YYYY is the year, MM is the numeric month, DD is the day, and HHMMSS is
the time). This naming is implemented to capture both the acquisition time and date, as well as
to ensure uniqueness in the naming. When running a batch, a sub-folder with the “Batch Name”
is created in the “Test-Files” data directory on disk. The individual tests in the batch will be
saved to that sub-folder with the naming format of “QC-XXX.fcs” where XXX is a sequential
number starting at “000” and incrementing with each test run in the batch. The “Sample ID” field
can be used as general text description of the sample/test being run. This field can be changed
by touching in the box and using the keyboard below to type a description. The Sample ID field
can be changed for each/any test in the batch.
Notes: 1.) Capital letters can be typed by hitting
the “CAPS” key to change the keyboard to capital letters. 2.) Additional punctuation/symbols
are available by touching “Punc”.
The “Dilution Factor” field is a drop-down list with the options
of entering “1:1” (no dilution), “1:2” (2x dilution), “1:5” (5x dilution), and “1:10” (10x dilution).
Note: the value selected here will automatically be applied to all tests’ count outputs to
adjust the measured counts for the dilution level, providing the true (dilution adjusted)
initial starting concentration.
The value is set by touching the box (image below/right) and
touching one of the dropdown entries. The “Enter” button can then be touched to start the
test/batch. For the first test after the system is powered-up, the system will perform the laser-
homing routine (as described in the laser/cassette alignment section). For every subsequent
