Gambro COBE 2991, Essentials Manual

Page: 221 / 273

Loading the Starting Cell Product
COBE 2991 Cell Processor • Essentials Guide 6-23
P
rocessing
and C
o
mmon
Operations
6
18
If desired, rinse the cell product bag (to avoid cell loss) by doing the following:
a Place a clamp on the clear tubing between the junction manifold and the
RCD.
b Place the cell product bag on the tabletop (Figure 6-17 on page 6-19).
c Press the PREDILUTE button and allow the desired volume of
processing/wash solution to fill the cell product bag.
d Press the STOP RESET button and manually agitate the cell product
bag.
e Hang the cell product bag on the left hanger bar.
f Press the BLOOD IN button, let the cell product bag empty, then press
the STOP RESET button to close valve V1.
19
Reset the CENTRIFUGE SPEED control to the appropriate setting,
according to the protocol.
20
Proceed to “Processing” on page 6-26.
Loading Cell Product Using the Blue-Striped Tubing
For protocols requiring the addition of processing/wash solutions through valves
V1, V2, and V3, the starting cell product can be loaded through the blue-striped
tubing (see Figure 6-14 on page 6-15) using the following procedure:
1
Ensure that the blue-striped tubing is not loaded into the collect valve (if
applicable). To open the collect valve, press the TUBE LOAD button, set the
VALVE SELECTOR to V1, and place the COLLECT/SOV button (toggle
switch) in the COLLECT position.
2
Ensure that the additional processing/wash solution is connected to the red-
striped tubing.
3
Close the slide clamp on the blue-striped tubing.
4
Remove the cap from the spike on the blue-striped tubing. Insert the spike
into the cell product bag.