Gambro COBE 2991, Essentials Manual

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1-24 COBE 2991 Cell Processor • Essentials Guide
Introduction
Agitate/Wash-In Operation
The Agitate/Wash-In operation (Figure 1-18) follows the Supernatant-Out
operation. The centrifuge decelerates to a near stop. The hydraulic pump reverses to
its home (starting) position, removing hydraulic fluid from underneath the flexible
diaphragm. The selected valve opens and the centrifuge begins to agitate.
Processing/wash solution flows by gravity into the cell processing bag as the
oscillating motion of the centrifuge causes the cell product and the processing/wash
solution to mix. The COBE 2991 advances from the Agitate/Wash-In operation to
the next cycle when
• The hydraulic pump has returned to its home (starting) position.
-and-
• The selected MINIMUM AGITATE TIME has elapsed.
-or-
• The START SPIN button is pressed.
This concludes the first cycle.
See Table 1-6 on page 1-25 for a summary of the Agitate/Wash-In operation.