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Mini MIRI
®
Dry and Mini MIRI
®
Humidity Multiroom IVF incubators User Manual
Rev. 3.0
65
•
Methanol 10 ppm v/v max.
•
Hydrogen Cyanide 0.5 ppm v/v max.
•
Total Sulphur (as S) 0.1 ppm v/v max.
35.2 Gas supply N
2
For the regulation to maintain the correct O
2
concentration levels in the Mini MIRI
®
Dry
or Mini MIRI
®
Humidity multiroom IVF incubator compartments, the device must be con-
nected to a stable source of 100% N
2
at 0.4
–
0.6 bar (5.80
–
8.70 PSI) of pressure.
Measure the N
2
concentration in the gas supply by routing the gas line into a bottle with-
out a lid and a suitably large opening. Set the pressure/flow so that the bottle is flushed
continually with gas, without increasing pressure in the bottle (i.e., the amount of gas ex-
iting the bottle should be equal to the gas volume entering the bottle).
Sample from the bottle near the bottom with the gas analyzer.
A gas analyzer that can measure 0% O
2
accurately can be used. 100% N
2
= 0 %
O
2
.
PASS: N
2
concentration measured must be between 95.0%
–
100%.
The use of N
2
gas with moisture will damage the flow sensors. Moisture level
must be verified on the gas manufacturer's certificate: only 0.0 ppm v/v Max is per-
missible.
35.2.1 About N
2
Nitrogen makes up a significant portion of the earth's atmosphere with 78.08% by vol-
ume. Nitrogen is a colorless, odorless, tasteless, non-toxic, and almost inert gas. Nitrogen
is principally shipped and used in either gaseous or liquid form.
N
2
gas can act as a simple asphyxiant by displacing air.
The User should make sure the N
2
used is safe and moisture-free. Below is a list of some
standard component concentrations. Please note that the values given are NOT the
proper amounts, only an example:
•
Research Grade 99.9995%.
•
Contaminant.
•
Argon (Ar) 5.0 ppm.