Mini MIRI
®
Dry and Mini MIRI
®
Humidity Multiroom IVF incubators User Manual
Rev. 3.0
64
Sample from the bottle near the bottom with the gas analyzer.
PASS: CO
2
concentration measured must be between 98.0% - 100%.
Use of CO
2
gas with moisture will damage the flow sensors. Moisture level
must be verified on the gas manufacturer's certificate: only 0.0 ppm v/v Max is per-
missible.
35.1.1 About CO
2
Carbon dioxide (CO
2
) is a colorless, odorless, non-combustible gas. Carbon dioxide above
the triple point temperature of -56.6 °C and below the critical point temperature of 31.1
°C can exist in both a gaseous and a liquid state.
Bulk liquid carbon dioxide is commonly maintained as a refrigerated liquid and vapor at
pressures between 1,230 kPa (approx. 12 bar) and 2,557 kPa (approx. 25 bar). Carbon
dioxide may also exist as a white opaque solid with a temperature of -78.5 °C under at-
mospheric pressure.
A high concentration of carbon dioxide (10.0% or more) can asphyxiate
quickly without warning with no possibility of self
–
rescue regardless of the oxygen
concentration.
The User should make sure the CO
2
used is safe and moisture-free. Below is a list of some
common component concentrations. Please note that the values given are NOT the
proper amounts, only an example:
•
Assay 99.9% v/v min.
•
Moisture 50 ppm v/v max. (20 ppm w/w max).
•
Ammonia 2.5 ppm v/v max.
•
Oxygen 30 ppm v/v max.
•
Oxides of Nitrogen (NO/NO
2
) 2.5 ppm v/v max each.
•
The non-volatile residue (particulates) 10 ppm w/w max.
•
The non-volatile organic residue (oil and grease) 5 ppm w/w max.
•
Phosphine 0.3 ppm v/v max.
•
Total volatile hydrocarbons (calculated as methane) 50 ppm v/v max. of which 20
ppm v/v.
•
Acetaldehyde 0.2 ppm v/v max.
•
Benzene 0.02 ppm v/v max.
•
Carbon Monoxide 10 ppm v/v max.
