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SECTION 6
NOZZLE MAINTENANCE AND PERFORMANCE
6.5 Using the Line Flush
This procedure may be performed when troubleshooting a staining issue. It is also required when
switching from carbol fuchsin to fluorescence staining reagents. This procedure requires the
Nozzle Maintenance kit. You can flush the B or C line separately or both lines at the same time.
The B-line must be flushed at least once per month when using potassium permanganate.
NOTE:
This procedure requires a minimum of 1 hour and at least 250 mL of cleaning solution
(diluted SS-029C or SS-266).
NOTE:
A carousel must be in place during the procedure or the instrument will generate an error
and abort the procedure.
1
Remove the B or C nozzle, or both, depending on the desired line flush.
2
From the Maintenance menu, press
Line Flush
.
3
Select the desired line or lines to be flushed.
4
Load 500 mL of deionized or distilled water into the each line you are
flushing.
5
Insert an empty carousel and close the lid.
6
Press
Start
. Follow the prompts on the display to complete the line flush.
The instrument will pump approximately 400 mL of water through the
line(s). A status bar will indicate progress.
7
When the flush is complete, remove the remaining water.
8
Load 200 mL of the prepared cleaning solution (diluted SS-029C or SS-266)
into the line(s).
9
Press
Start
. The instrument will pump approximately 100 mL of the
prepared cleaning solution through the line(s) and will then start a 1-hour
countdown timer.
10 Wait for the timer to complete, or allow the instrument to remain idle up
to a maximum of 12 hours.
