26
SECTION 2
SETUP AND PREPARATION FOR OPERATION
2.1 Instrument Setup
Installing Standard 500 mL Reagent Bottles
WARNING!
Reagents used in the instrument contain moderately hazardous chemicals that require
care in handling. Always handle reagents using appropriate safety measures, including
gloves and eye protection.
NOTE:
Reagents should be stored according to the conditions specified on their label.
After opening, reagents are stable until the expiration date specified on the label unless
otherwise indicated by the symbol shown at left.
1 Place the reagent bottles front to back according to the particular staining
option desired. Be sure reagents are in the correct position.
Switching
between staining options may require instrument programming changes
(Section 3), specific combinations of reagents (Appendix D), and cleaning
certain reagent lines.
CAUTION:
When switching between carbol fuchsin and fluorescence staining the user
must perform a B-Line Flush (Section 6.5) before installing potassium
permanganate.
CAUTION:
Users must adjust the stain programming, depending upon the type of staining
(carbol fuchsin or fluorescence) desired, which reagents are being used and
user preferences. Refer to Appendix D for complete information.
CAUTION:
The stainer cannot detect which reagents are loaded on the instrument. The
user must take care to load the correct reagents in the correct positions, based
upon the options given here and user preference.
WARNING!
Each new stainer ships with a pink label attached to the B reagent line. This
label indicates that the B-line must be flushed with 100 mL of DI water before
installing potassium permanganate.
2
Place each 500 mL reagent bottle in the correct position.
(A) Decolorizer
(B) Counterstain
(C) Primary Stain
(D) Deionized or Distilled Water
(E) Approved Alcohol
A B C D E
