29
SECTION 2
SETUP AND PREPARATION FOR OPERATION
2.2 Preparing the Stainer for Operation
Priming Procedures
NOTE:
The instrument is shipped with alcohol in the reagent lines. For proper
performance, this alcohol must be replaced with the correct reagent for each reagent
line prior to use.
Thoroughly purge and prime each reagent delivery line using the following instructions.
1
Remove each spray nozzle with the provided nozzle tool by turning
counterclockwise.
2
Note the location of each nozzle so you can return it to the original
position during reassembly.
3 Place a carousel on the stainer hub to prevent stain from entering the
motor shaft.
CAUTION:
Fluid from priming can flood and damage the motor if the drain tube is not
properly installed.
4
Press
Maintenance
from the Main menu.
5
Press the
A
prime button. Stain should appear within 10 seconds. When
properly primed, a steady stream of reagent (no sputtering or breaks)
flows from the nozzle receptacle.
•
If stain appears, proceed to the next step.
•
If stain does not appear within 10 seconds, perform the manual
priming procedure in Section 6.3.
CAUTION:
Never operate a dry pump for more than 10 seconds. Operating a dry pump
may cause damage to the instrument.
6 Repeat the previous steps for each nozzle (B, C, D, and E). When the D
prime button is pressed, most of the reagent will come out of the D
R
position. With nozzles installed, reagent should spray equally from D
R
and
D
F
.
D
R
* E C B A D
F
**
* D
R
= D Rear
**D
F
= D Front