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SECTION 4
OPERATING THE STAINER
4.1 Operating Instructions
Using Blocking Slides
If the carousel is not full, blank slides should be used as blocking slides. Blocking slides
prevent overspray of reagents onto the specimen slides. Overspray can cause slides to
become over-decolorized.
•
Place a blocking slide in front of position 1 and 2.
Figure 12: Using Blocking Slides
B
–
Blocking Slide
1
–
Slide Position 1
2
–
Slide Position 2
3
–
Slide Position 3
4
–
Slide Position 4
Performing a Stain Cycle
NOTE:
Using this stainer requires specific sample pre-treatment and fixation steps
(Appendix E). It also requires specific combinations of reagents to be loaded on the
stainer (Appendix D) in combination with programming adjustments (Section 3).
1
Insert a carousel with specimen slides and close the instrument lid.
2
If you have not enabled Slide Tracking, select the number of slides to be
stained. Slide selection defaults to full carousel at the end of the run, after
pressing Stop, or selecting a number greater than the full carousel default.
NOTE:
To stain an odd number of specimen slides, select the next higher
number listed on the display. For example: to stain 3 slides, select 4. To stain 7
slides select 8, etc.
If you are using the optional barcode reader, the number of slides is
programmed automatically. See Enable Stain Slide Tracking in Section 3.1.
NOTE:
Do not count blocking slides as part of the total number of slides.
B
B
2
1
3
4
