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SECTION 6
NOZZLE MAINTENANCE AND PERFORMANCE
6.3 Manual Priming
1
Remove the carousel from the bowl.
2
Remove the nozzle connected to the reagent line to be manually primed.
3
Insert the priming tool nozzle adapter (included in the Nozzle Maintenance
Kit) into the nozzle holder.
4
Turn the nozzle adapter clockwise to install the adapter into the holder.
5
Withdraw the priming tool plunger halfway to create a vacuum. Hold the
plunger in position.
6
Press
Maintenance
from the Main menu.
7
Press
Volume Test
.
8
Press the desired prime button to start the reagent pump.
9
Run the reagent into the tube until the fluid is free of bubbles, then press
Stop
.
WARNING!
Do not pull the plunger completely out of the priming tool. Pulling the
plunger out of the tool may result in splashing or spraying of reagents. Do not
push the plunger in while it is connected to the nozzle holder.
10 Turn the nozzle adapter counterclockwise to remove it from the nozzle
holder.
11 Discard the collected fluid into the stainer bowl.
12 Perform the 60 Second Prime function on the affected reagent line to
remove any remaining air in the pump or reagent line.
NOTE:
This requires at least 250 mL of reagent. Make sure there is enough
reagent in the selected bottle before starting this function.
13 Reinstall the nozzle.
14 Perform the Hub Pattern Test and record results.
15 Perform the Volume Test and record results.
