Dionex ASE 150, Operator'S Manual

Page: 60 / 176

ASE 150 Operator’s Manual
52
Doc. 065207-02 9/08
5. To save the method:
a. Press an arrow button to move the cursor to
SAVE
and press
ENTER
.
b. To save the modified version of the method to the current method number,
press
ENTER
.
NOTE Saving to the current method number will
permanently overwrite the previous version of the
method.
c. To save to a different method number, press an arrow button to select the
desired number and press
ENTER
.
d. Press
MENU
to exit the
METHOD EDITOR
screen and return to the
MENU
screen.
3.7 Developing a New Method
Follow the procedure below to develop a method for a new sample type.
1. Select a solvent (see Section 3.1.1 ).
2. Prepare the sample (see Section 3.1.3 ).
3. Edit a custom method (see Section 3.6 ).
4. Run the new method three times (with the same cell and sample, into three
separate collection vessels) and analyze the extracts.
5. If target analytes are present in extract 2 or 3, make the adjustments listed
below (one at a time) to the method. After each adjustment, repeat the method
and analyze the extract.
a. Raise the temperature. In general, raising the temperature increases the
efficiency of the extraction process. However, because compounds can
degrade at high temperatures, it is advisable to raise the temperature in
small increments (10 to 20 °C). The maximum allowable temperature of
the ASE 150 is 200 °C. If oxidation is a concern, degas the solvent before
use.
b. Run two static/rinse cycles. Extending the static time enhances diffusion
of the analytes into the extraction fluid. Separating the static time into two
cycles, instead of using one longer cycle, allows the introduction of fresh