14
5.3
Peristaltic pump
The sources are pumped from their containers through the open valves of the manifold by the main pump. The
main pump is a 12 roller, high precision peristaltic pump, capable of handling up to 12 channels simultaneously,
providing accurate and identical dispensing across channels.
Figure 14: Peristaltic Pump (Only 8 channels shown)
The main body of the pump (1), is fixed to the machine chassis. The special tubing (3) setup is facilitated by
individual cartridges (2). Cartridges snap in and out of the pump by pressing on the release lever (4). A detailed
description of tubing setup can be found in section 7.5.
5.4
Perifusion chamber
Sources delivered by the pump will react with the cells inside the perifusion chamber. The cells are contained
inside the chamber within a gel bead matrix. A fiberglass filter is used as a bottom layer before the output.
Secretions will come out the outlet tube for collection in the well-plate. The internal volume is 275 µL. The
chamber is translucent to allow visualization of its contents. It is designed to fit a micro-centrifuge tube for easy
cell recovery. This is important at the time of data normalization. A detailed description of the chamber setup for
an experiment can be found in section 7.4.
1. Top cap
2. O-ring
3. Cell chamber
4. Fiberglass filter
5. Bottom cap
6. Micro-centrifuge tube
Summary of Contents for PERI-4.2
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