10
4.3
Perifusion chamber auxiliary rack
The auxiliary rack is included with every Perifusion system
to assist in the preparation of the Perifusion chambers. The
rack can hold up to 8 chambers at a time for cell loading
outside the machine. This allows the scientist to do the
preparation away from the machine in a more comfortable
position.
Figure 7: Perifusion Chamber Rack
4.4
Perifusion Nozzles
The Biorep® Perifusion Nozzle reduces the droplet size dispensed into the 96-well
plates. Small droplets (~4µL) ensure optimal resolution of the dynamic insulin
response.
Figure 8: Perifusion Nozzles
4.5
Collection well-plates
The collection well-plate is where the cell secretions and
the perfusate are collected for analysis. The Perifusion
system was designed to work with standard 96 well-plates,
and now has the ability to use high capacity, deep well
plates for longer experiments.
Figure 9: Collection Well-plate
4.6
Bio-Gel P-4
The Bio-Gel P-4 polyacrylamide beads are used at the time
of Perifusion chamber setup. They are used contain the
cells and prevent clogging of the filter.
See appendix A for beads solution preparation.
Figure 10: Bio-Rad polyacrylamide beads
4.7
Fiberglass pre-filters
Summary of Contents for PERI-4.2
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