27
Cooling
1. To set up the tray cooling, fill a container with coolant. The coolant
can be chilled water or a mixture of water and alcohol
2. Connect 1/8" I.D. silicone tubing as shown on the figure
3. Prime the diaphragm pump using a siphon or syringe
4. Press "TRAY PUMP" to turn the pump ON. Once the tray pump is
enabled, the button will turn blue.
Figure 35: Cooling
WARNING: Do not run pump dry!
7.10 Protocol execution
When you reach this screen the cells should be in the chambers, the manifold and should be primed with the
input solutions and the temperature of the incubator and tray should be at their set points. On this screen you can
start your Perifusion experiment by hitting the PLAY button. The flow rate, manifold state, and movement of the
tray will be automatically handled by the machine. The machine will only require your input in special cases (as
described in previous sections), or when the machine requires manual change of the sample tray. This screen is
primarily used, as its name suggests, to monitor experiment progress.
Summary of Contents for PERI-4.2
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