35
Bead Suspension Preparation
Add beads to 5ml mark of a 50ml conical
Add 40ml of buffer solution.
Prepare bead-buffer suspension at least one day before the experiment to
allow beads to absorb buffer.
Keep the bead-buffer suspension refrigerated
(4
℃
)
Bead suspension may be kept refrigerated for up to 4 weeks
Bead Suspension Use
Follow these instructions before using the bead suspension in a perifusion chamber preparation:
Warm bead suspension to 37
℃
using an incubator or water bath.
Do not add cold bead
suspension to cells, since this may adversely affect your experiment
.
Agitate conical thoroughly before pipetting bead suspension to add into perifusion columns. This
ensures that approximately even number of beads are present in each column.
Summary of Contents for PERI-4.2
Page 30: ...30 AUC Area Under Curve...
