11
A fiberglass filter sheet is included with every perifusion
system. A hole puncher (included) is used to make round
cut-outs of the filter sheet to place inside the perifusion
chamber during its set-up. The purpose of this filter is to
retain the chamber contents while allowing the flow of the
perfusate.
Figure 11: Hole Punch
5
Perifusion system overview
5.1
Basic Perifusion schematic
The following schematic shows the basic functional elements and stages of the Perifusion system. Potential users
should become familiar with all the components of the system before attempting to operate the machine.
Figure 12: Basic Perifusion Schematic
1.
SOURCES
: Up to eight different test solutions can be used as sources in a single experiment. These sources
can be administered either manually by the user or automatically by the machine through the valve manifold.
Sources are contained inside the enclosure’s temperature controlled environment.
2.
VALVE
MANIFOLD
: An automated valve manifold handles the source selection for each channel. The
manifold has 8 input
channels (1, 2, 3…8) and four output channels A, B, C and D. Each output channel is tripled,
for a total of 12 output connections. A detailed description can be found in section 5.2.
3. MAIN PUMP:
A high precision, 12- channel peristaltic pump moves the test compounds from the source,
through the manifold, to the Perifusion chambers and out to the well plate.
4. PERIFUSION CHAMBER:
The Perifusion chambers hold the cells to be stimulated. The input to each chamber
is the source selected by the manifold for its respective channel. The output will contain the secretions and
metabolites to be collected on the well plate. Up to eight Perifusion chambers can be used in parallel.
5. TEMPERATURE CONTROL:
All the test sources are housed in the same incubator enclosure as the
Perifusion chambers to provide a controlled temperature environment for the entire process. A multi-point
circulation design ensures temperature uniformity within 1ºC around the Perifusion chambers.
Summary of Contents for PERI-4.2
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