6
2
Introduction
Congratulations on your purchase of the Biorep Perifusion system. This is the newest generation of a product that
has received countless hours of development to include all the features necessary to perform the most controlled
and precise stimulated cell secretion experiments in the field. You will not be disappointed with the value and
efficiency this machine will bring to your research.
The Perifusion system accurately stimulates and collects the secretions of pancreatic islets in-vitro. Its use has
been extended to include the measurement of secretions of differentiated cells derived from pancreatic progenitor
stem cells. Coupled to a tandem mass spectrometer, the Perifusion system can help identify molecules secreted
during the stimulation of pancreatic islets.
The machine accurately controls temperature, flow, stimulus source and sample collection in up to 8 channels in
parallel. The Perifusion system is designed to execute pre-programmed protocols that can last for hours and
require several sample trays.
This User Manual is intended to be used by scientists, researchers, and technicians who have received training in
cell perifusion methods and technologies. This document contains the necessary information for installing and
operating the machine. However, it is not meant to provide scientific guidance on perifusion protocols. Any
perifusion protocols encountered in this manual will be solely for the purpose of illustration of machine operation
and should not be interpreted otherwise.
3 Perfusion
5 Purification
6 Perifusion
7 Cell Transport
8 Transplant
1 Donnor
2 Organ Transportation
4 Isolation
Figure 2 Perifusion System in the islet isolation process
Summary of Contents for PERI-4.2
Page 30: ...30 AUC Area Under Curve...