Biorep Technologies PERI-4.2, User Manual

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4 Accessories
A complete set of accessories is included with the machine. For ordering additional or replacement accessories
please consult our webpage
www.biorep.com or contact one of our sales representatives at (305) 330-4449.
4.1 Tubing Set
The Perifusion tubing set delivers the stimulus to the cell chamber while minimizing dead volume. The perifusion
tubing set is made out of 2 types of tubing: A- Connection tubing, which is for the perifusion fluid handling system
(source-manifold, manifold-pump, pump-chamber); and B- the peristaltic pump tubing, which provides the precise
resolution needed in perifusion experiments. A set of each type is included with this machine.
Item Description Reference
A Silicone Tubing; 0.040in ID, 0.085in OD 89068-462
B Two-Stop Tubing (Tygon R-3603) (orange-green);
0.015in ID, 0.035in WT;
PERI-TUB-12
Table 2: Items Included in the Perifusion Tubing Set
4.2 Perifusion Chamber
The Perifusion chamber is where all cell reactions will take place. Substances will be delivered through the inlet
tube, cells contained in the chamber will react to the substances, and secretions will come out the outlet tube for
collection. A new system includes 16 perifusion chambers.
1- Inlet tube
2- Top cap
3- Top O-Ring
4- Cell chamber
5- Bottom O-Ring + Filter
6- Bottom cap
7- Outlet tube
Figure 6: Perifusion Chamber