bioMerieux PREVI Color Gram User Manual Download Page 34 | Manualshive

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Operating the PREVI™ Color Gram

Other settings

4-14

PREVI™ Color Gram

User’s Manual

Press

Press

Enter the required
DECOLORIZER level.

Example: for a very
thick specimen press

See "Starting a
staining cycle",
page 4-8.

DECOLORIZER 3

FULL CAROUSEL

PROGRAM

1 = DECOLORIZER
2 = FIXATION
3 = ADJUST STAIN

DECOLORIZER 3

SELECT 1 - 9

DECOLORIZER 8

FULL CAROUSEL

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Summary of Contents for PREVI Color Gram

Page 1: ...501 1689 en IVD bioM rieux S A 69280 Marcy l Etoile France Tel 33 0 4 78 87 20 00 Fax 33 0 4 78 87 20 90 http www biomerieux com Printed in France 673 620 399 RCS Lyon REF 29675 version B 03 2009 2008...

Page 2: ...re subject to a proper and skilled use of the product in strict conformance with the User s Manual Photo and illustration credit bioM rieux bioM rieux the blue logo and PREVI are used pending and or r...

Page 3: ...he list of revisions below summarizes replacements or additional pages in your User s Manual Version Date of printing Modifications Pages modified A 09 2008 Creation All B 03 2009 Addendum 4501 1842 i...

Page 4: ......

Page 5: ...on 2 6 Other parts 2 6 3 Installing the PREVI Color Gram 3 1 Unpacking procedure 3 1 Installation procedure 3 2 Installing waste tubing 3 2 Connecting power 3 3 Installing reagent bottles 3 4 Preparat...

Page 6: ...0 B line flushing procedure 5 13 Priming all lines simultaneously 5 16 Long term storage 5 16 Cleaning 5 18 Cleaning the instrument and the carousel 5 18 Liquid spills 5 18 Preventing injury 5 18 Disi...

Page 7: ...ighly flammable A break or malfunction in the reagent delivery system has the potential of releasing up to 500 ml of alcohol in and around the instrument If this occurs carefully shut off the power to...

Page 8: ...ntrained personnel should not attempt to dismantle the instrument Only spare parts supplied or specified by bioM rieux should be used Fitting of non approved parts may affect the performance and safet...

Page 9: ...taining productivity 12 or 30 slides at a time CLEAN cycle to purge each spray nozzle with ethanol or methanol 9 operator selected decolorizer cycles separate reservoir delivery tube pump and spray no...

Page 10: ...00 m Pollution degree 2 Over voltage category II Performance PARAMETERS CHARACTERISTICS Reagent spray nozzles Each reagent has a separate spray nozzle to dispense the correct amount of reagent Reagent...

Page 11: ...hout alcohol fixation 237 4 min 69 320 5 3 min 113 3 Total with alcohol fixation 307 5 1 min 144 402 6 7 min 116 8 Option for ethanol or methanol fixation R e f e r e n c e s PRODUCTS REFERENCES PREVI...

Page 12: ...for 5 reagent channels Fuchsin or safranin with or without acetone Iodine Crystal Violet Distilled water Ethanol or methanol Front panel and keypad description Indicators Power Rotation Cytocentrifuge...

Page 13: ...Waste tubing Power cord Bowl Spray nozzle orifices DR Distilled water Rear E Ethanol or methanol C Crystal Violet B Iodine A Fuchsin or Safranin with without acetone DF Distilled water Front Drive hub...

Page 14: ...anol or methanol can be hazardous and should be handled with the same care The concentrated nozzle cleaning solution must be stored at 15 25 C until the expiry date indicated on the bottle label After...

Page 15: ...he instrument with at least 30 cm 12 inches clearance from obstructions or hazardous materials U n p a c k i n g p r o c e d u r e Open the 2 boxes and remove the protection foam packaging Check the c...

Page 16: ...ty of condensation causing a hazardous situation Keep the waste container tube straight and as short as possible 2 meters 79 inches maximum The waste container tube should be positioned at a lower lev...

Page 17: ...certain the switch is OFF O Check that the voltage label complies with the power supply Insert the electric cord into the power socket Plug the power cord into the mains AC power Turn the power switc...

Page 18: ...distilled water Place each bottle in the appropriate position Open the new bottle record the letter on the cap and keep it for future use e g long term storage Right side panel with 5 prime buttons fo...

Page 19: ...re the 1 st use of the instrument WARNING Never operate a dry pump for more than 10 seconds Nozzle checking Remove each spray nozzle with the nozzle tool by turning the counter clockwise Note the loca...

Page 20: ...les Replace each spray nozzle by hand and then with the nozzle tool by turning clockwise Pattern and volume testing For first use please refer to both of the following tests The Pattern test page 4 3...

Page 21: ...PREVI Color Gram routine process This routine process flow diagram describes the necessary stages for the routine use of the instrument Details are given in the following sections Key Orange line run...

Page 22: ...agent delivery line using the following instructions WARNING Never operate a dry pump for more than 10 seconds The CLEAN cycle is a two stage process that re primes reagents into the nozzles then uses...

Page 23: ...RIZER 3 FULL CAROUSEL PRIME 15 Perform a PATTERN test Note If a CLEAN cycle is aborted a warning message will be displayed until the CLEAN cycle is completed P a t t e r n t e s t Press Press DECOLORI...

Page 24: ...old a sheet of white paper in front of nozzle A Press Check the quality of the pattern If the pattern is not correct see Nozzle handling page 5 4 Repeat steps 3 4 and 5 for all the other nozzles DF DR...

Page 25: ...rrous metal objects on the lab bench These can be attracted to the magnets on the bottom of the carousel and cause damage if spun free of the magnets during spinning of the carousel Slides must be loa...

Page 26: ...the slides into the carousel The slides must be inserted diametrically opposite one to the other to balance the carousel Be sure that slides are loaded in balanced pairs If an odd number of slides ne...

Page 27: ...PREVI Color Gram User s Manual 4 7 Replace the lid by pressing the button and lowering the lid over the indexing posts Release the button and press the lid handle until it clicks into place Place the...

Page 28: ...te container is full empty it see Emptying the waste container page 4 13 WARNING bioM rieux recommends the use of the default DECOLORIZER setting IMPORTANT Use as an emergency stop when required e g a...

Page 29: ...accordance with good laboratory practice guidelines and local regulations Remove the carousel from the bowl Remove the carousel lid by pressing the button and lifting the lid away Carefully remove ea...

Page 30: ...st before restarting the instrument This stage requires the use of 10 ml of alcohol divided equally between each nozzle Be sure the stainer light is ON Place the empty carousel in the instrument and c...

Page 31: ...fixation mode and not the NORMAL mode ALCOHOL fixation in normal mode is shorter and may lead to specimen loss The ALCOHOL FIXATION is OFF by default Press Press Press See Starting a staining cycle pa...

Page 32: ...bove the maximum safety level When the waste container is full empty it see Emptying the waste container page 4 13 IMPORTANT Do not transfer residual reagent from a used bottle to a fresh bottle Doing...

Page 33: ...container once a week see Maintenance and cleaning page 5 1 O t h e r s e t t i n g s WARNING The information contained in this section is intended for customers who wish to use this equipment with ap...

Page 34: ...ser s Manual Press Press Enter the required DECOLORIZER level Example for a very thick specimen press See Starting a staining cycle page 4 8 DECOLORIZER 3 FULL CAROUSEL PROGRAM 1 DECOLORIZER 2 FIXATIO...

Page 35: ...ded setting is Medium A setting of reagent C High and reagent B High will produce darker Gram positive organisms A setting of reagent C Low and reagent B Low will produce lighter Gram positive organis...

Page 36: ......

Page 37: ...ve staining runs If the PREVI Color Gram is not used for more than one week the long term storage procedure must be applied see Long term storage page 5 16 P r e v e n t i v e m a i n t e n a n c e p...

Page 38: ...and can be switched off Spray and wipe the interior of the bowl and the nozzles with ethanol 70 Clean the instrument lid with ethanol 70 Check that the maintenance procedures have been completed by f...

Page 39: ...hat the maintenance procedures have been completed by following the Preventive maintenance chart page 7 1 and then sign with your initials WARNING WASTE DISPOSAL Dispose of used or unused reagents as...

Page 40: ...on are required IMPORTANT If the compression screw cannot be easily loosened use light penetrating oil and a 5 8 inch wrench to loosen the nozzle Perform the clean see CLEAN alcohol purge stage page 4...

Page 41: ...ml centrifuge tube position DF Note the reagent letter on the tube Fill with 25 ml of cleaning solution and cap the tube Shake the tube at least 10 times Soak the parts as long as possible from 10 min...

Page 42: ...ur thumb to prevent nozzle loss Inspect nozzle parts Remove any material in the swirl cone grooves by sliding a piece of paper along the edge edge Rinse parts in the tube with distilled water Rinse wi...

Page 43: ...ression screw Hold all the parts in a vertical position until reassembly is completed Return the nozzle to its original location Repeat instructions 1 to 8 for each nozzle Prime each reagent channel P...

Page 44: ...the nozzle connected to the air locked pump Screw the nozzle adapter of the unclogging tool included in the nozzle maintenance kit into the nozzle holder Withdraw the unclogging tool plunger to creat...

Page 45: ...be withdrawn further to accept more fluid Do not pull the plunger completely out of the barrel of the tool Remove the nozzle adapter tubing and express the collected fluid into the stainer bowl Reins...

Page 46: ...t must be performed weekly Press Press DECOLORIZER 3 FULL CAROUSEL 1 PATTERN TEST 2 VOLUME TEST 3 B LINE FLUSH 4 PRIME LINES VOLUME TEST PRESS ANY PRIME BUTTON Hold a volume test tube small tube in pl...

Page 47: ...ance range too low loosen the nozzle slightly too high tighten the nozzle slightly And repeat the test Tolerance Tube Minimum Maximum A 10 ml 12 ml B C 9 ml 11 ml DF DR E 9 ml If the volume is in the...

Page 48: ...uture use empty the contents of the tubes into the bowl clean the tubes rinse the tubes with distilled water waste liquid thrown in the bowl put the tubes back into their original place in the main te...

Page 49: ...IMPORTANT A carousel must be in place during the procedure otherwise it will generate an error and will not continue the process Remove the nozzle B Perform a B line flush by pressing Press DECOLORIZE...

Page 50: ...in the B line position and press Note pump approx 100 ml of the cleaning solution through the B line Allow to sit for at least 1 hour up to 12 hours if possible Note in the meantime centrifuge mode c...

Page 51: ...ote pump approx 400 ml of distilled water through the B line Load 200 ml of Iodine and press Reinstall the nozzle B Press B LINE FLUSH DI WATER B 30 LOAD 500 ML DI WATER ON B PRESS RUN B LINE FLUSH IO...

Page 52: ...1 PATTERN TEST 2 VOLUME TEST 3 B LINE FLUSH 4 PRIME LINES PRIME LINES PRESS ANY PRIME BUTTON L o n g t e r m s t o r a g e If the PREVI Color Gram is inactive for more than a week perform the followin...

Page 53: ...r methanol in the line Remove and clean the nozzles see Disassembly page 5 4 and Cleaning page 5 5 IMPORTANT Leave flushing fluids in the reagent lines during storage Do not run reagent lines dry Flus...

Page 54: ...ctious it should be disinfected in accordance with any local applicable regulations CAUTION For disinfection solutions and procedure see Disinfecting and or sterilizing page 5 19 Note These decontamin...

Page 55: ...o the instrument Failure to use slides in good condition can lead to breakage during the cycle Make certain there are no small ferrous metal objects in the bowl These can be attracted to the magnets o...

Page 56: ...in a biological safety hood or well ventilated area Spray the inner bowl and inner lid with a disinfection solution Repeat the spray treatment every 2 or 3 minutes Leave the solution on surfaces for...

Page 57: ...amage the internal electronics Repeat wipe down every 2 or 3 minutes for about 10 minutes total Remove the decontamination solution by thoroughly wiping surfaces with a cloth soaked in water Immerse o...

Page 58: ...rable to its original packaging Shipping conditions Temperature 0 to 40 C Humidity 80 non condensing Disinfection declaration The following disinfection declaration MUST be printed and completed by th...

Page 59: ...ndicate a serious internal problem For continued protection replace only with fuses of the type and rating indicated on the rear panel of the stainer Put the power switch in position O Disconnect the...

Page 60: ......

Page 61: ...r cord from the power entry module then opening the fuse door For continued protection replace only with fuses of the type and rating indicated on the rear panel of the stainer CAUTION Fuse failure ma...

Page 62: ...use A small puddle of stain around the drain opening at the rear of the bowl or a few drops of stain around the bottom of the bowl is not unusual and will not cause a problem If the bowl is filling wi...

Page 63: ...at the tubing is not cracked or deformed If the problem persists please contact your bioM rieux representative STAINING The stainer fails to spray reagent during a staining cycle and or continues to r...

Page 64: ...he slides in the correct positions as indicated by the markings on the carousel If the problem persists please contact your bioM rieux representative Abnormal staining on entire surface of some slides...

Page 65: ...Try to make your smears as thin as possible for a given specimen to minimize fixation problems Be sure to use clean premium quality slides If you are using the instrument s alcohol fixation function...

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Page 67: ...PREVI Color Gram User s Manual 7 1 7 Preventive maintenance chart...

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