PREVI™ Color Gram
User’s Manual
2-1
2 . G e n e r a l
F u n c t i o n a l d e s c r i p t i o n
The
PREVI™ Color Gram
is designed to Gram stain specimen smears on microscope slides.
A Gram stain aids in the presumptive diagnosis of the illness, and it gives preliminary
classification of the causative agent.
This microprocessor-controlled slide stainer is dual purpose. Additionally, the
PREVI™ Color Cytocentrifuge Rotor
allows to quickly sediment sample cells onto
microscope slides for staining or other purposes.
The chemical mechanism of Gram differentiation is based on the differential cell wall
permeability to the Crystal Violet-Iodine complex. The Gram stain yields a specimen in which
the Gram-positive organisms are purple to black and the Gram-negative organisms are pink
to red.
K e y f e a t u r e s
PREVI™ Color Gram
’s features are:
•
minimized reagent consumption
•
fast staining
•
high volume staining productivity (12 or 30 slides at a time)
•
CLEAN cycle to purge each spray nozzle with ethanol or methanol
•
9 operator-selected decolorizer cycles*
•
separate reservoir, delivery tube, pump and spray nozzle for each reagent
•
operator-selectable ethanol or methanol fixation function to fix samples.
The correct accessory must be used for each function.
The
PREVI™ Color Cytocentrifuge Rotor
is available from bioMérieux as an option offering
additional features.
*
program no. 2 and 3 recommended by bioMérieux.
I n t e n d e d u s e
The
PREVI™ Color Gram
is an
in vitro
diagnostic medical device for professional use only.
The
PREVI™ Color Gram
has been designed to perform automated Gram staining of human
sample micro-organisms for
in vitro
diagnostics with bioMérieux reagents only.
The
PREVI™ Color Gram
has been qualified with the following cell suspensions:
•
blood
•
bronchoalveolar liquid (BAL)
•
cerebrospinal fluid (CSF)
•
feces
•
sputum
•
vaginal swab
•
urine.
WARNING!
bioMérieux
disclaims
any responsibility for the use of the PREVI™ Color Gram with
biological cell suspensions other than those mentioned above.