Bioer GeneQ Thermal Cycler, Manual, Page: 18 / 33

Gene Q Thermal Cycler Chapter 4 Operation Guide
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Hotlid=off 2008-11-01 10:05:00
100
75
50
25
0
Temp 1c 95.0 2c 55.0 3c 72.0 4 04.0
Time 01:00 02:00 01:45 00:00
Seg. Temp(C) Time Ramp(C/s) +Temp +Time
1c 9 5.0 01:00 #.# +0.0 +0:00
2c 55.0 02:00 #.# +0.0 +0:00
3c 72.0 01:45 #.# +0.0 +0:00
4 04.0 00:00 #.# +0.0 +0:00
Cycle1
：
×10 From 01 to 03
+Seg. +Cycle Delete Back Save/Run
Note
：
The sign “*” at the top of User and File indicates the current running file is default
one, which can be modified in the parameter setting interface.
In this interface, you can edit sections and
segments of the file. A File is comprised by
sections and segments. And the sections
contain many segments, which you can set
cyclers to be run (refer to the figure). Move
cursor by pressing “
Up/Down ” and “ Right
/Left ”, and the position that the cursor
reaches will be displayed in white on a black
background. Press “
Digit & Letter ” to modify
parameter settings. Press “ +/- ” to /-.
 Press “ +Seg. ” to enter segment edit
interface. In every segment, you can set
setting temperature (Temp), duration
(Time),
Ramping rate (Ramp),
temperature increment of every cycler
(+Temp) and time increment of every
cycler (+Time). Press “
Delete ” to delete
the present segment.
 Press “ +Cycle ” to enter segment edit interface, where you can set cycle numbers and initial
cycle segment. The figure shows a cycle from the first segment to the third one, and there are
totally 10 times cycles.
 Press “ +Cycle ” repeatedly to add sections (max.: 5 sections ). Press “ Delete ” to delete the
present segment. Move cursor by pressing “ Up/Down ” to enter the former segment or the
latter.
 Press “ Save ” to enter file save interface.
 Press “ Back ” to go back to File List Interface.
Special Warning:
1. For the new edited PCR program, please save first then run it, otherwise the file file can’t be
run;
2. When setting “Time” as “--:--”, the hotlid will be closed automatically when the block reaches
the target temperature.
File Edit Interface
Relation of sections and segments in a file
File
section 1
Cycle1 ×** From ** to **
Seg. 1
Seg. 2
Seg. 16
section 2
Cycle2 ×** From ** to **
·
·
·
·
·
·
section 5
Cycle5 ×** From ** to **