Applied Biosystems GeneAmp PCR System 9600, User Manual

Page: 88 / 296

3-9
A Look at the CYCL Program
In the following steps, you will look at the main displays in the
CYCL program. In each display, you will see the default
parameters (e.g., times and temperatures) that make up a
common PCR cycle. Using the
STEP key, you will quickly step
through all the displays and accept the default values. Keep in
mind, however, that default values can be changed, which you
will do later on in this tutorial.
1 The first screen in the CYCL program should still be on your
display:
This display sets the number of setpoints or incubation
temperatures in one cycle. The cursor marks the default value, 3,
which specifies three-temperature PCR cycling.
2 Press STEP . This accepts the default value of three set-points and
moves you to the next display:
This display sets the ramp time, temperature, and hold time for
Setpoint 1 — typically used for template denaturation.
The temperature of the first PCR setpoint is 94 degrees C and is
held for 30 seconds.
3 Temperature PCR
Cursor
Setpt #1 Ramp 0:00
94.0C Hold 0:30
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