Applied Biosystems GeneAmp PCR System 9600, User Manual

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♦
Enter the temperature increment in decimal form,
except you do not type a decimal point. For
example, to specify a two-degree increment,
press
2 , then 0 . Then press ENTER .
OR
♦
Press ENTER to accept the default (0.0)
temperature. This will not change the specified
temperature of Setpoint #1 in successive cycles.
The cursor moves to the hold time increment.
3 Press the OPTION key to change the arrow direction,
indicating whether you want an increase or decrease
in the hold time, then press
ENTER .
4 Enter the time by which the hold time will increase or
decrease, or accept the default increment (0:00),
which indicates that the hold time will not change.
Use the numeric keys to enter the time, then press
ENTER .
These two displays are used to set the temperature
and hold time for the second setpoint (Setpt #2) and
change the time and temperature values in
successive cycles, if desired. For a three-
temperature PCR, the default temperature is 55
degrees C. For a two-temperature PCR, the default
is 68 degrees C.
To change these displays, use the same procedure
as described for Setpoint #1.
Setpt #2 55.0C
Hold for 0:30
Setpt #2 55.0C
Change time/temp? NO
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