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Ankom TDF, Operator'S Manual

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Ankom TDF Operator'S Manual Download Page 7

 

Operator’s

 

Manual

 

 

 

 

 

 

 

 

 

Rev

 

A

 

4/30/12

  

   

pg.

 

7

 

 

External

 

Components

 

–

 

Front

 

View

 

 

 

 

 

 

 

Touch

 

Screen

 

Display

 

Filtrate

 

Cup

 

(#1

 

of

 

6)

 

Waste

 

Tray

 

Clamp

 

Bar

 

D

 

Clamp

 

Bar

 

C

 

Heating

 

Paddle

 

(#2

 

of

 

6)

 

Mixing

 

Pad

 

(#3

 

of

 

6)

 

SDF

 

Station

 

(#6

 

of

 

6)

 

IDF

 

Station

 

(#4

 

of

 

6)

 

SDF

 

Delivery

 

Nozzle

       

(#1

 

of

 

6)

 

Clamp

 

Bar

 

A

 

Clamp

 

Bar

 

B

 

Control

 

Buttons

 

Hook

 

for

 

SDF

 

Bag

 

(#2

 

of

 

6)

 

IDF

 

Delivery

 

Nozzle

     

(#1

 

of

 

6)

 

Control

 

Panel

 

Front

 

Cover

 

(Clear)

 

Summary of Contents for TDF

Page 1: ...This page intentionally left blank Operator s Manual Rev A 4 30 12 2052 O Neil Rd Macedon NY 14502 Telephone 315 986 8090 Fax 315 986 8091 www ankom com ...

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Page 3: ... 15 Analysis Options using the ANKOMTDF Dietary Fiber Analyzer 15 IDF SDF Analysis 17 TDF Analysis 30 Protein Determination 43 Ash Determination 46 Enhance your productivity 49 Understand the Status Screen 53 Fault Handling 56 Diagnostics Mode 59 Periodic Maintenance 71 Troubleshooting 71 Appendix A Reagents AOAC 991 43 AACC 32 07 01 NMKL 129 2003 72 Appendix B Storage Procedure 74 Appendix C Part...

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Page 5: ...g you to achieve the best possible results Please review the entire manual before you begin operating this product Warranty ANKOM Technology warrants the ANKOMTDF Dietary Fiber Analyzer against any defects in workmanship or material for one year after the original date of purchase This warranty does not include damage to the instrument resulting from neglect or misuse During the warranty period sh...

Page 6: ... capture the appropriate fiber particles while allowing all non fiber components to pass through The instrument will use an IDF bag and an SDF bag when configured to recover IDF and SDF residue The instrument will use an IDF Flow Thru bag and an SDF bag when configured to recover TDF residue The automated fiber recovery is achieved by first digesting samples within bags using enzyme treatments Dep...

Page 7: ...lay Filtrate Cup 1 of 6 Waste Tray Clamp Bar D Clamp Bar C Heating Paddle 2 of 6 Mixing Pad 3 of 6 SDF Station 6 of 6 IDF Station 4 of 6 SDF Delivery Nozzle 1 of 6 Clamp Bar A Clamp Bar B Control Buttons Hook for SDF Bag 2 of 6 IDF Delivery Nozzle 1 of 6 Control Panel Front Cover Clear ...

Page 8: ...nents Right Side View Control Panel 95 EtOH Container HCl Container Enzyme Containers Nitrogen Supply Port 100 psi 6 9 bar Front Cover Arm Power Switch Front Cover 78 EtOH Container Buffer Container Distilled Water Container Front Cover Arm Chemical Container Holder HCl Container Holder ...

Page 9: ...re Sensors Waste Valves 1 6 Main Electrical Cabinet Clamp Bar Cylinder 1 of 8 In Line Heater t Manifold Water Buffer 78 EtOH 95 EtOH Supply Valves IDF Inlet Valve Stations 4 5 6 SDF Inlet Valve Stations 4 5 6 IDF Inlet Valve Stations 1 2 3 SDF Inlet Valve Stations 1 2 3 Enzyme Supply Valves HCl Supply Valve ...

Page 10: ...ts when operating this instrument Do NOT heat seal or place filter bags in an oven until all acetone has evaporated Handle Sodium Azide with caution only after reviewing MSDS Do NOT add Sodium Azide to a solution until the pH has been adjusted Failure to observe this caution may be hazardous to your health WARNING Attempts to override safety features or to use this instrument in a manner not speci...

Page 11: ...om and a sleeve that forms the body of the container Remove the top and the sleeve of the instrument shipping container Lift the instrument off of the shipping container bottom and place it on a surface that is firm and level in an area near a Nitrogen Supply and a drain or chemical waste container Waste fluids are drained from the instrument using gravity Therefore position the instrument so that...

Page 12: ...led Water 1 Buffer 1 78 EtOH 1 95 EtOH Chemical Container Holders 1 single container holder 1 three container holder 3 Connect the Chemical Container Holders to the feet of the instrument Remove the nuts from the outside of the left and right feet of the instrument Slide the small single container holder onto the bolts on the left foot and slide the large three container holder onto the bolts on t...

Page 13: ...ointing away from you slide the tray under the front of the instrument until it can t go back any farther The side ridges of the tray will contact the front face of the steel frame 6 Connect the Drain Tube Connect and secure the Drain Tube to the Waste Tray and run it to either a drain or a waste container avoiding twists in the tube If necessary cut the Drain Tube and use the elbow connector to m...

Page 14: ...ions 8 Connect the Chemical Containers to the corresponding tubes The Chemical Containers and associated tubes are all labeled and color coded for easy connection using push on connectors Make sure that you hear a click sound when you connect the tubes to the containers 9 Connect the Enzyme Containers to the corresponding ports The Enzyme Containers connect into labeled and color coded ports on th...

Page 15: ...d SDF residue or to recover TDF residue To maximize productivity this instrument also allows a user to start new procedures IDF SDF and TDF before the previous ones have completed The following describes these analyses along with their specific filter bag configurations Analysis Name Analysis Description Upper Bag Lower Bag IDF SDF This analysis is used to determine IDF and SDF residue separately ...

Page 16: ...Operator s Manual pg 16 Rev A 4 30 12 This page intentionally left blank ...

Page 17: ... manual To match the enzyme requirements of the approved method dilute the standard enzymes referred to in the method with a solution of 25 glycerol and 0 02 Sodium Azide in Distilled Water as follows Enzyme Dilution Alpha amylase Megazyme Dilute 1 25 ml of amylase to 25 ml Protease Megazyme Dilute 2 5 ml of protease to 25 ml Amyloglucosidase AMG Megazyme Dilute 5 0 ml of AMG to 25 ml Hazardous Ma...

Page 18: ... Bag Weigh Holder should be placed in the center of the scale for best results 5 Weigh Diatomaceous Earth DE DE is used during fiber analysis to enhance the flocculation and filtration of the SDF fraction Place ca 1 g of DE in each of six tared tins and record the weights 6 Weigh Samples Place 0 5 0 05 g of sample in each of six tared tins and record the weights 7 Install SDF filter bags on the AN...

Page 19: ... IDF bag can fit inside the top of the SDF bag 7 4 With the bag being held by the Delivery Nozzle center it horizontally between the lines on the back part of Clamp Bar C 7 5 Repeat steps 7 2 7 4 for all six stations 7 6 Re install Clamp Bar D 7 7 Flatten the bags to remove any wrinkles With fingers away from the clamp bars press the Clamp Bar D button on the control panel to pinch the bags just a...

Page 20: ...part of Clamp Bar A For proper mixing during the IDF process the IDF bags must be horizontally centered over the Heating Paddles and between the Centering Lines on the back part of Clamp Bar A 9 4 Place at least 20 mm 0 75 inches of the filter section of the IDF bag inside the top of the SDF bag to allow for the flow of liquid into the SDF bag after the IDF process is complete 9 5 Repeat steps 9 1...

Page 21: ...y important to keep it below the tip of the Delivery Nozzle so that it can be properly rinsed 10 2 Rinse the tin with no more than 3 ml of Distilled Water to ensure complete transfer 10 3 Repeat steps 10 1 10 2 for all IDF stations 10 4 Re install Clamp Bar A 11 Perform the IDF SDF procedure using the ANKOMTDF Dietary Fiber Analyzer The Control Panel on the ANKOMTDF Dietary Fiber Analyzer uses Tou...

Page 22: ...cedure The following screen will be displayed 11 4 Notice that the procedure you selected is now displayed in the top right corner of the screen along with the function Make sure that your SDF bags are installed then press the check mark button The following screen will be displayed The Clamp Bar D button on the Control Panel is now disabled until the SDF process completes SDF Bags installed AOAC ...

Page 23: ...your IDF bags are installed then press the check mark button The following screen will be displayed 11 7 Make sure that your samples have been placed in your IDF bags then press the check mark button The following screen will be displayed Samples in IDF Bags AOAC 991 43 IDF SDF IDF Bags installed AOAC 991 43 IDF SDF Diatomaceous Earth added in SDF Bags AOAC 991 43 IDF SDF ...

Page 24: ...amples take different amounts of time to filter the above screen allows you to set your filter times To change any of the times shown on the screen press the specific gray button A number pad will be displayed that will allow you to enter the time that you want When all of the times shown on the screen are what you want press the check mark button The following screen will be displayed Filter minu...

Page 25: ... test to check for leaks in the tubing For the first run after a power up cycle or for the first run after the instrument has sat idle for twelve hours the following status screen will be displayed If the fluid lines are already charged properly or when the line charge is complete the following status screen will be displayed Status Overall Progress Action in progress IDF Time remaining Lines Char...

Page 26: ...nsed to avoid loss of sample If you add acid or base to adjust the pH you must mix the solution in order to get an accurate pH reading To manually mix the solution follow the steps below 12 1 Move the pH probe from side to side 12 2 Using your fingers press the outside of the bag just above the clamp bar multiple times to mix the solution 12 3 When you have completed the pH measurement process pre...

Page 27: ...he bags on the Rinse Stand by sliding the back part of each bag under the pinch mechanism Keep the top of the bag open 13 3 Using a wash bottle squirt acetone into each bag making sure that all residue is rinsed down into the filter 13 4 Repeat step 13 3 so that each bag gets rinsed a total of two times 13 5 Allow acetone to evaporate from the bags Hazardous Materials Do NOT heat seal or place bag...

Page 28: ... bag before determining the protein content 14 7 Place each bag in the Drying Rack 15 Dry the IDF and SDF filter bags 15 1 Make sure your oven is 105 C at the location where the bags are placed 15 2 Place the Drying Rack with the filter bags in the oven and dry to constant weight about 90 minutes 15 3 When dry remove all IDF and SDF filter bags from the oven and place them in separate desiccant po...

Page 29: ...nk Filter Bag g 21 Calculate the SDF value SDF R1 R2 2 P A B M1 M2 2 fF1 fS1 D1 fF2 fS2 D2 2 P1 A2 D2 B M1 M2 2 Where M1 M2 Original wt of duplicate samples adjusted for pre treatment fat and sugar losses g R1 R2 Residue for duplicate samples g fF Final Filter Bag with residue g fS Initial Filter Bag g D Original wt of Diatomaceous Earth g P Protein of residue and bag g A Ash of residue and bag g ...

Page 30: ...Operator s Manual pg 30 Rev A 4 30 12 This page intentionally left blank ...

Page 31: ...hemicals and enzymes is located in Appendix A of this manual To match the enzyme requirements of the approved method dilute the standard enzymes referred to in the method with a solution of 25 glycerol and 0 02 Sodium Azide in Distilled Water as follows Enzyme Dilution Alpha amylase Megazyme Dilute 1 25 ml of amylase to 25 ml Protease Megazyme Dilute 2 5 ml of protease to 25 ml Amyloglucosidase AM...

Page 32: ... Bag Weigh Holder should be placed in the center of the scale for best results 5 Weigh Diatomaceous Earth DE DE is used during fiber analysis to enhance the flocculation and filtration of the SDF fraction Place ca 1 g of DE in each of six tared tins and record the weights 6 Weigh Samples Place 0 5 0 05 g of sample in each of six tared tins and record the weights 7 Install SDF filter bags on the AN...

Page 33: ... Thru bag can fit inside the top of the SDF bag 7 4 With the bag being held by the Delivery Nozzle center it horizontally between the Centering Lines on the back part of Clamp Bar C 7 5 Repeat steps 7 2 7 4 for all six SDF stations 7 6 Re install Clamp Bar D 7 7 Flatten the bags to remove any wrinkles With fingers away from the clamp bars press the Clamp Bar D button on the control panel to pinch ...

Page 34: ...ing the digestion process the IDF Flow Thru bags must be horizontally centered over the Heating Paddles and between the Centering Lines on the back part of Clamp Bar A 9 4 Place at least 20 mm 0 75 inches of the bottom of the IDF Flow Thru bag inside the top of the SDF bag to allow for the flow of solution into the SDF bag after the digestion process is complete 9 5 Repeat steps 9 1 9 4 for all si...

Page 35: ...e so that it can be properly rinsed 10 2 Rinse the tin with no more than 3 ml of Distilled Water to ensure complete transfer 10 3 Repeat steps 10 1 10 2 for all IDF stations 10 4 Re install Clamp Bar A 11 Perform the TDF procedure using the ANKOMTDF Dietary Fiber Analyzer The Control Panel on the ANKOMTDF Dietary Fiber Analyzer uses Touch Screen technology To operate the machine you will press ide...

Page 36: ...ssay The following screen will be displayed 11 4 Notice that the procedure you selected is now displayed in the top right corner of the screen along with the function Make sure that your SDF bags are installed then press the check mark button The following screen will be displayed The Clamp Bar D button on the Control Panel is now disabled until the SDF process completes SDF Bags installed AOAC 99...

Page 37: ...ow Thru bags are installed then press the check mark button The following screen will be displayed 11 7 Make sure that your samples have been placed in your IDF Flow Thru bags then press the check mark button The following screen will be displayed Samples in IDF Flow Thru Bags AOAC 991 43 TDF IDF Flow Thru Bags installed AOAC 991 43 TDF Diatomaceous Earth added in SDF Bags AOAC 991 43 TDF ...

Page 38: ... be displayed 11 10 Because different samples take different amounts of time to filter the above screen allows you to set your filter times To change any of the times shown on the screen press the specific gray button A number pad will be displayed that will allow you to enter the time that you want When all of the times shown on the screen are what you want press the check mark button The followi...

Page 39: ...t to check for leaks in the tubing For the first run after a power up cycle or for the first run after the instrument has sat idle for twelve hours the following status screen will be displayed If the fluid lines are already charged properly or when the line charge is complete the following status screen will be displayed Status Overall Progress Action in progress IDF Time remaining Lines Charging...

Page 40: ...easily rinsed to avoid loss of sample If you add acid or base to adjust the pH you must mix the solution in order to get an accurate pH reading To manually mix the solution follow the steps below 12 1 Move the pH probe from side to side 12 2 Using your fingers press the outside of the bag just above the clamp bar multiple times to mix the solution 12 3 When you have completed the pH measurement pr...

Page 41: ...ng a wash bottle spray acetone into each bag making sure that all residue is rinsed down into the filter 13 4 Repeat step 13 3 so that each bag gets rinsed a total of two times 13 5 Allow acetone to evaporate from the bags Hazardous Materials Do NOT heat seal or place bags in an oven until all acetone has evaporated 13 6 With your Heat Sealer set between 3 and 4 seal each bag just above the filter...

Page 42: ...DF residue See the Ash Determination section of this manual for recommended procedures 18 Calculate the TDF value TDF R1 R2 2 P A B M1 M2 2 fF1 fS1 D1 fF2 fS2 D2 2 P1 A2 D2 B M1 M2 2 Where M1 M2 Original wt of duplicate samples adjusted for pre treatment fat and sugar losses g R1 R2 Residue for duplicate samples g fF Final Filter Bag with residue g fS Initial Filter Bag g D Original wt of Diatomac...

Page 43: ...erial from the filter bag and run only the filter through the Kjeldahl instrument To determine the protein content within the IDF residue follow the procedure below 1 Place a clean blank sheet of paper down on the bench 2 Working on top of the clean sheet of paper cut the filter bag just above the seal at the top of the filter making sure that any loose residue from the bag is collected on the bla...

Page 44: ...ping the residue in the filter material 8 With your Heat Sealer set to 6 press the arm down for 3 to 4 seconds to seal the filter 9 Label the folded sealed filter 10 Repeat steps 1 9 for all IDF bags that will be used for determining protein content 11 Run a Kjeldahl procedure on each filter using 17 ml of H2SO4 10 g of K2SO4 and 40 mg of CuSO4 5H2O in a system with 250 ml tubes that have a 42 mm ...

Page 45: ...ress the arm down for 3 to 4 seconds to seal the polypropylene edge of the filter bag 4 Label the folded sealed filter 5 Repeat steps 1 4 for all SDF bags that will be used for determining protein content 6 Run a Kjeldahl procedure on each filter bag using 27 ml of H2SO4 10 g of K2SO4 and 30 mg of CuSO4 5H2O in a system with 250 ml tubes that have a 42 mm diameter Pellets can be used if an equival...

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Page 47: ...n fold the bag in thirds from side to side 2 With your Heat Sealer set to 6 press the Heat Sealer arm down for 3 to 4 seconds to seal the clear polypropylene part of the bag just above the white filter material 3 Label the folded sealed filter bag and place it in a labeled ashing crucible 4 Repeat steps 1 3 for all IDF bags to be ashed 5 Place covered ashing crucibles with filter bags in a furnace...

Page 48: ... bag fold the bag upward leaving at least 5 mm at the top of the clear polypropylene that is not folded Then fold the bag in thirds from side to side 2 Set your Heat Sealer to 6 and press the Heat Sealer arm down for 3 to 4 seconds to seal the unfolded polypropylene to the folded part of the bag 3 Label the folded sealed filter bag and place it in a labeled ashing crucible 4 Repeat steps 1 3 for a...

Page 49: ...ns When a digestion process completes one of the following screens is displayed showing that the process in the top bag is complete At this time you can remove the IDF or IDF Flow Thru bag from the instrument then press the Start New Procedure button on the screen The following screen will be displayed Select a Procedure AOAC 991 43 IDF SDF TDF Status Overall Progress Action in progress IDF Time r...

Page 50: ... Press the Clamp Bar B button on the Control Panel to pinch the bag just above the filter for IDF bags 2 Check Fluid Levels To ensure that you have enough fluids to run a complete IDF SDF or TDF procedure you must begin with fluid levels above the Minimum Level lines on the chemical containers and at least 10 ml of each enzyme Add fluids and enzymes as necessary If a precipitation process is still...

Page 51: ...nswer the questions 5 3 When you have answered all of the questions one of the following screens will be displayed 5 4 The instrument is now completely set up and ready to automatically run another digestion process Press the START button to begin Depending on the procedure you are beginning and the procedure that is currently in process one of the following status screens will be displayed Press ...

Page 52: ...empty into the SDF bag until you verify that a new SDF bag has been installed This verification occurs when you press the SDF bag installed button on the display after the current precipitation process completes AOAC 991 43 TDF Status pH required Yes Overall Progress Action in progress IDF Time remaining Checking tube integrity Please wait XX XX XX SDF Time remaining SDF Action XX XX XX Time remai...

Page 53: ... the word Yes is displayed in box 1 until the pH has been checked at which time the word Complete is displayed Otherwise the word No is displayed in this box 2 Function and Procedure Box 2 displays AOAC 991 43 along with the most recent procedure that you have started either IDF SDF or TDF For example AOAC 991 43 IDF SDF is displayed when the most recent procedure started is an IDF SDF If you star...

Page 54: ...is only displayed when an ABORT operation is allowed 11 FAULT Button 11 is displayed if a fault occurs within the instrument When button 11 is pressed the Fault Screen is displayed This screen has information about all faults that have not been cleared See the Fault Handling section of this manual for more information 12 Display Temperatures When button 12 is pressed temperatures of the In line He...

Page 55: ...ation bypass Button 15 is displayed when a top or bottom process begins its filter action or when a bottom process begins its precipitation phase During the filter actions the button reads Filter bypass During a precipitation phase the button reads Precipitation bypass When pressed button 15 instructs the instrument to stop the filter or precipitation action and start the next action If you mistak...

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Page 57: ...a number in each section of the screen Your computer screen will NOT show these numbers 1 Fault description Box 1 contains the description of a fault that has recently occurred 2 Reset fault after it has been repaired Button 2 is only displayed when a low input pressure fault occurs When button 2 is pressed the fault is reset 3 Next fault Multiple faults can occur during a procedure Pressing butto...

Page 58: ...evice that heats the fluid before delivery is reporting a temperature that is lower than the specified range for the SDF EtOH95 Delivery phase IDF process is aborted SDF process has not yet started Run diagnostics Replace sensor or heater E9 In line Heater Cooling Failure The device that heats the fluid before delivery is reporting a temperature that is higher than the specified range for the Wate...

Page 59: ...When you press the Pressures button on the Diagnostics screen the following screen is displayed The readings shown on this screen for the input pressure and the lines to all six stations represent the absolute pressure These values will dynamically change as the pressure changes Pressure Monitor psi xx x Line 1 xx x Line 2 xx x Line 3 xx x Line 4 xx x Line 5 xx x Line 6 xx x Input Pressure Diagnos...

Page 60: ... press the Set Date Time button on the Diagnostics screen the following screen is displayed Press the buttons on the screen above to set the date and time that is displayed on the Select a Function screen shown below Select a Function 06 30 2011 09 47 AM AOAC 991 43 Diagnostics Set Date Time Press button with number to change 14 2011 15 31 MONTH DAY YEAR HOUR MINUTE 10 Temperature Monitor C XX Pad...

Page 61: ...In line Heater on and light the LED located below the Touch Screen Display on the Control Panel After turning on the heaters you can monitor them using the Temperatures diagnostic After completing the Heater Test you should turn all heaters off Faults When you press the Faults button on the Diagnostics screen the Faults screen is displayed See the Fault Handling section of this manual for more inf...

Page 62: ...ayed with the bottom part of the screen showing the status of each tube This diagnostic is automatically executed at the beginning of each new IDF SDF or TDF run Motor Test When you press the Motor Test button on the Diagnostics screen the following screen is displayed Motor Test Set Valves GO Motor Direction FWD 20 RPM 120 RPM 60 RPM 240 RPM Motor Speed 10 0 Amount ml All lines should be charged ...

Page 63: ...isplayed Running the Motor Test could leave fluid in some of the lines After running a Motor Test always run the Clear IDF SDF Lines diagnostic Clear IDF SDF Lines When you press the Clear IDF SDF Lines button on the Diagnostics screen the IDF and SDF lines are cleared of any fluids Before running this diagnostic place small waste cups at each IDF and SDF station under the nozzles and close the Fr...

Page 64: ...e digestion times so that you can run through the normal operation sequence without having to wait for the digestion processes to complete Because the computer control software uses these values during normal operation you must reset them to the factory defaults before running IDF SDF or TDF assays or you may get erroneous results If you start a new procedure with Digestion Times that are differen...

Page 65: ...luid in the lines for the next assay In that case you should use this diagnostic to re fill the lines that ran out Service Mode The Service Mode should only be used by authorized personnel When you press the Service Mode button on the Diagnostics screen the following screen is displayed Service Mode Volume Calibration When you press the Volume Calibration button the following screen is displayed V...

Page 66: ...eps below 1 Label and weigh six small empty glass beakers 2 Press the START button on the screen above The following screen is displayed 3 Install extra plastic containers under all six IDF Delivery Nozzles to catch any fluid currently in the lines With containers in place press the OK button After fluid empties into the containers the following screen is displayed Place extra containers under IDF...

Page 67: ...ll six IDF Delivery Nozzles and press the OK button While the fluids are being delivered the following screen is displayed When the fluid delivery is complete the following screen is displayed Volume Calibration Final fluid delivery in progress Place Calibration containers under IDF Delivery Nozzles Press OK when done OK ...

Page 68: ...hen you press the Temperature Sensor Corrections button on the Service Mode screen the following screen is displayed This diagnostic allows you to set offsets to correct for differences in temperature sensors Corrections to temperature sensors should only be made after installing a new Mixing Pad assembly TDF33 Service Mode IDF Count When you press the IDF Count button on the Service Mode screen t...

Page 69: ... see the number of digestion operations that have been done Expert Mode When Expert Mode is ON all of the question screens in the IDF SDF and TDF procedures are bypassed except for the Check pH manually and Filter minutes OK screens IDF Count Number of IDF runs XX ...

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Page 71: ...containers and filters 1 Inspect the containers and container filters at the end of the tubing inside each container checking for any precipitate particles or foreign matter that may be plugging the filters or settled at the bottom of the container 2 Clean the filters by running them under fast running water 3 Rinse the containers out with water and let air dry as needed Regularly clean the instru...

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Page 73: ...ycerol and 0 02 Sodium Azide in Distilled Water f Diatomaceous earth DE ANKOM XTC or equivalent g MES 2 N Morpholino ethanesulfonic acid No M 8250 Sigma Chemical Co or equivalent h TRIS Tris hydroxymethyl aminomethane No T 1503 Sigma Chemical Co or equivalent i MES TRIS buffer solution 0 05M MES 0 05M TRIS pH 8 2 at 24 C Dissolve 19 52 g MES and 12 2 g TRIS in 1 7 L H2O Adjust pH to 8 2 with 6N Na...

Page 74: ...nes All of the water will run to waste 4 Empty all chemical and enzyme containers 5 Run the Line Charge ALL diagnostic to pump air into the supply lines to clear them Any water in the lines will run to waste 6 To clear the remaining lines from the manifold to waste follow the steps below 6 1 From the Motor Test diagnostic screen press the Amount button and set the number to 10 6 2 From the Motor T...

Page 75: ...F17 Control Panel assy TDF23 Front Clamp Bar assy TDF24 Paddle Bar assy TDF25 1 Burkert Valve assy Water TDF25 2 Burkert Valve assy Buffer TDF25 3 Burkert Valve assy EtOH78 TDF25 4 Burkert Valve assy EtOH95 TDF26 SMC Valve assy TDF27 Pump assy TDF31 Supply Manifold assy ...

Page 76: ... Check Valve assy TDF35 Amylase Container assy TDF36 Protease Container assy TDF37 AMG Container assy TDF38 HCl Container assy TDF39 Buffer Container assy TDF40 Distilled Water Container assy TDF41 78 EtOH Container assy TDF42 95 EtOH Container assy TDF44 Spray Tip assy ...

Page 77: ...k assy TDF51 Rinse Stand assy TDF52 Bag Weigh Holder assy TDF53 Filtrate Cup XTC Diatomaceous Earth DE F08 Solvent Resistant Marker X45 Desiccant Pouch 1915 Heat Sealer 8262 Gasket DF S SDF Filter Bags 50 DF I IDF Filter Bags 50 DF FT Flow thru Filter Bags ...

Page 78: ...mples at one time RF Gas Production System High sensitivity pressure measurement Applications include Biomass to Energy analysis e g Ethanol methane etc Biodegradability Ruminant Nutrition Yeast Activity Beer Wine Fermentation Soil respiration BOD Human Digestion etc Wireless Computer control and data storage Chemicals A wide variety of chemicals used for many different lab operations Pre mixed so...

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