Ankom TDF, Operator'S Manual

Page: 17 / 78

Operator’s Manual
Rev A 4/30/12 pg. 17
IDF/SDF Analysis
An IDF/SDF analysis measures the amount of IDF and SDF within a given sample. This requires the use of ANKOM DF-I and
DF-S filter bags. When starting a new run, the ANKOM
TDF
Dietary Fiber Analyzer must have bags installed at all stations (a
total of twelve bags).
To enhance the productivity of your instrument, you can begin the IDF process for
a new run while the SDF process of a previous run is finishing. See the “Enhance
your productivity” section of this manual for more details.
To perform an IDF/SDF analysis, follow the steps below.
1. Prepare chemicals and enzymes.
When using the ANKOM
TDF
Dietary Fiber Analyzer for the AOAC 991.43, AACC 32-07.01, and NMKL 129, 2003
methods use the chemicals and enzymes referred to therein. For your convenience the list of chemicals and enzymes is
located in Appendix A of this manual.
To match the enzyme requirements of the approved method, dilute the standard enzymes referred to in the method with a
solution of 25% glycerol and 0.02% Sodium Azide in Distilled Water as follows.
Enzyme Dilution
Alpha amylase (Megazyme) Dilute 1.25 ml of amylase to 25 ml
Protease (Megazyme) Dilute 2.5 ml of protease to 25 ml
Amyloglucosidase (AMG – Megazyme) Dilute 5.0 ml of AMG to 25 ml
Hazardous Materials –
Handle Sodium Azide with caution only after reviewing
MSDS. Do NOT add Sodium Azide to a solution until the pH has been adjusted.
To ensure that you have enough fluids to run a complete IDF/SDF procedure, you must begin with fluid levels above the
Minimum Level lines on the chemical containers and at least 10 ml of each enzyme. Add fluids and enzymes as
necessary.
Do NOT leave the enzyme ports on the instrument open to the air or the enzymes
in the valves may dry up and plug the ports.
2. De ‐ fat and de ‐ sugar your samples as needed according to the AOAC 991.43, AACC 32 ‐ 07.01, or
NMKL 129, 2003 methods.
3. Label the filter bags using a Solvent Resistant Marker.