Technical description
20
Edition 06/2015
InnuPure C16
Washing buffer transfer
Washing
Collection of the magnetic particles and transfer of the
excess
Washing of the nucleic acids
The magnetic particles remain in the work
cavity and the washing buffer is then trans-
ferred there. By pipetting different washing
buffers on and off the nucleic acids are
washed.
The number of washing steps and volume
and type of washing buffer depend on the
type of the source material used. In addition,
the buffers differ in the case of DNA or RNA
extraction.
Between each washing step the magnetic
particles are collected with the bound nucleic
acids from the cavity floor. The correspond-
ing wash excesses are pipetted off and re-
turned to their original holding position.
Tip washing
To prevent the carry-over of washing solution
residue, the tips used are flushed between
specific washing steps.
Removal of the washing solution from the 1st work
cavity
Discharge of the washing solution into the 4th work
cavity
Change of the work cavity
Prior to the final washing step the washing
solution is removed completely from the 1st
work cavity and transferred by pipetting over
into the 4th work cavity.
The quality of the already washed nucleic
acids is significantly improved by the transfer
into a clean work cavity. Any lysis residue at
the walls is retained in the "dirty" cavity and
only the already washed nucleic acids bound
to the magnetic particles are transferred on.
Ethanol removal
The removal of ethanol residue at the mag-
netic particles and within the samples takes
place via a drying step. In this step, a heating
is activated in the cavity floor, causing the
ethanol residue to evaporate.
