Technical description
InnuPure C16
Edition 06/2015
15
4
Technical description
4.1
Basic system design
The InnuPure C16 is a flexible and efficient extraction system for the fully automated isola-
tion and purification of nucleic acid. The system, developed and produced in Germany, has
been designed for low sample throughput. A large selection of different source materials can
be used. The system combines a unique fluid handling technology with an extremely fast
walk-away principle.
The InnuPure C16 is equipped with pre-installed extraction protocols to prevent time-
consuming programming. The high degree of flexibility offered by the InnuPure C16 enables
the parallel isolation of DNA and RNA from up to 16 samples. The work-intensive process of
sample lysis which so far mostly had to be performed separately is now part of the automatic
extraction process (dependent on the source material).
The nucleic acids to be isolated adsorb at surface-functionalized magnetic particles. The
necessary extraction chemicals are added manually as necessary and enable the purifica-
tion of ultra-pure nucleic acids with excellent yields.
The extraction principle effectively prevents cross contamination, a problem often occurring
in vacuum-based extraction methods. The isolated nucleic acids can then be used immedi-
ately for further downstream applications.
Different specially optimized extraction kits are available for the InnuPure C16:
isolation of genomic DNA
viral or bacterial nucleic acids
processing of forensic samples
processing of food samples
processing of plant samples
PME-Extraction of cell free fluids
Extraction with bisulfite conversion
Depending on the application, the extractions take between 28 and 128 minutes.
4.2
Design, connections, indications
The InnuPure C16 is a stand-alone device. The operation and device control take place via
the control unit HID-Pro 320 attached to the front. Sample processing follows pre-installed
protocols tailored to the extraction kits. The kits include all reagents necessary for extraction,
permitting extraction to run fully automated. During operation the front door of the InnuPure
C16 is closed. For the status control of the device the touch sensor has been equipped with
an LED strip. This illuminates dependent on the operating state green or red or indicates the
use of the touch sensor by alternating green/red flashing.