AMA Instruments The GC 5000 BTX, Operating Manual, Page: 126 / 152

Operating Manual GC 5000 BTX; Version 5 – 11/2010 124
How to prepare and cut a capillary column in the right way:
1. Cut the ends square with none of the polyimide coating projecting
beyond the ends.
2. Inspect the ends with a 20X magnifier to make sure the cut is square.
3. In case the end is not square, make a small scratch about 1-inch from
the end with a diamond-tipped or ceramic scriber.
4. Therefore hold the column between the thumb and the forefinger, one
hand on either side of the scratch.
5. Place the scratch away from you and apply slight pressure with your
thumbs.
The column should snap easily and cleanly.
Note : Avoid making any scratches elsewhere on the column as this can
cause your column to break.
How to repair a broken column by using a capillary column
connector:
Universal capillary connectors provide a convenient method to connect a
capillary column together without any introduction of dead volume or active
sites.
1. One end at a time, firmly press the end of each column into the opening
in the capillary column connector.
A complete and uniform ring should be visible where the polyimide of
the column is in contact with the glass from the fitting.
2. If not, remove the column, re-cut the column, and re-install.
A leak-tight connection is formed between the polyimide coating and
the fused silica connector.