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Agilent 1260 Infinity II Preparative Autosampler User Manual

Agilent Technologies

6
Optimizing Performance

Optimization for Lowest Carry-over

Using the Automated Needle Wash (Wash Port)

Using an Injector Program

100

General Recommendation to Lowest Carry-Over

101

Fast Injection Cycle and Low Delay Volume

102

Overlapped Injection Mode

102

General Recommendations for Fast Injection Cycle Times

103

Precise Injection Volume

This chapter provides information on how to optimize the module.

Summary of Contents for 1260 Infinity II

Page 1: ...Agilent Technologies Agilent InfinityLab LC Series 1260 Infinity II Preparative Autosampler User Manual ...

Page 2: ...ibed in this document are furnished under a license and may be used or copied only in accordance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or sub contract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 1...

Page 3: ...odule This chapter provides information on how to use the module 4 Installation of the Optional Sample Cooler This chapter describes the installation of the optional sample cooler 5 Preparing the Module This chapter explains the operational parameters of the module 6 Optimizing Performance This chapter provides information on how to optimize the module 7 Troubleshooting and Diagnostics Overview ab...

Page 4: ... for maintenance 11 Identifying Cables This chapter provides information on cables used with the Agilent InfinityLab LC Series modules 12 Hardware Information This chapter describes the detector in more detail on hardware and electronics 13 LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC 14 Appendix This chapter provides addition informati...

Page 5: ... Performance Specifications 30 Specifications of the Sample Cooler 32 3 Using the Module 35 Magnets 36 Turn on off 37 Status Indicators 39 Exchange Drawers 40 Install the External Tray 42 Sample Trays 44 Choice of Vials and Caps 48 Transporting the Sampler with a Sample Cooler Installed 52 Agilent Local Control Modules 54 4 Installation of the Optional Sample Cooler 57 Injector Step Commands 58 Da...

Page 6: ...or Lowest Carry over 98 Fast Injection Cycle and Low Delay Volume 102 Precise Injection Volume 104 Choice of Rotor Seal 106 7 Troubleshooting and Diagnostics 107 Overview of the Module s Indicators and Test Functions 108 Maintenance Functions 110 Step Commands 119 Tests 120 Troubleshooting 121 Troubleshooting Guide for the Sample Transport Assembly 122 Agilent Lab Advisor Software 125 8 Error Info...

Page 7: ...tor Seal 167 Exchange the Metering Seal and Piston 173 Exchange the Gripper Arm 177 Replace the Peristaltic Pump Cartridge 179 Exchange the Wash Port Assembly 182 Replace the Module Firmware 187 10 Parts and Materials for Maintenance 189 Main Assemblies 190 Standard Parts 191 Drawer Assembly 192 External Tray 194 Analytical Head Assembly 900 µL 195 Standard Sampler Accessory Kit 196 Multi Draw Kit...

Page 8: ...N Configuration 231 What You Have to Do First 232 TCP IP parameter configuration 233 Configuration Switches 234 Initialization Mode Selection 235 Dynamic Host Configuration Protocol DHCP 237 Manual Configuration 240 PC and Agilent ChemStation Setup 244 14 Appendix 253 General Safety Information 254 Waste Electrical and Electronic Equipment Directive 261 Refrigerant 262 Radio Interference 264 Sound...

Page 9: ...n Product Description 10 Features 11 Overview of the Module 12 Operating Principle 13 Sampling Sequence 13 Needle Parkstation 18 Hydraulic Box 19 Transport Assembly 21 Leak and Waste Handling 22 Leak Sensor 22 Waste Concept 23 This chapter gives an introduction to the module and instrument overview ...

Page 10: ...mpler is designed for laboratories that need automated sample injection to increase daily throughput of purified sample This autosampler is easy to use reliable and capable of injecting sample volumes from vials in the microliter to milliliter range Figure 1 Overview of the sampler Status indicator Needle assembly Drawer Column shelf Leak drain Power switch ...

Page 11: ...ernal volume means minimum contribution to total internal volume of the system Instrument efficiency Fully automated vial sampler for reliable injection of sample volumes from 0 1 μL to 3 6 mL Support of flow rates up to 100 mL min at 400 bar Laboratory efficiency Maximum capacity of 132 sample vials within the same foot print Flexible and convenient sample handling with different types of sample ...

Page 12: ...icked up by the gripper arm and positioned below the needle station The gripper transport mechanism the needle station and the hydraulic unit are driven by motors Movement is monitored by optical sensors and optical encoders to ensure correct operation The metering device is always flushed after injection to ensure minimum carry over The 1260 Infinity II Preparative Autosampler injection head prov...

Page 13: ...is applied to the column when the injection valve returns to the mainpass position at the end of the sampling sequence The sampling sequence occurs in the following order 1 The injection valve switches to the bypass position 2 The piston of the metering device moves to the initialization position 3 The gripper arm moves from the home position and selects the vial At the same time the needle lifts ...

Page 14: ...mainpass position Injection Sequence Before the start of the injection sequence and during a preparative run the injection valve is in the mainpass position see Figure 2 on page 14 In this position the mobile phase flows through the autosamplers metering device sample loop and needle This ensures that all parts in contact with sample are flushed during the run thus minimizing carry over Figure 2 M...

Page 15: ...ins the valve unit switches to the bypass position see Figure 3 on page 15 Solvent from the pump enters the valve unit at port 1 and flows directly to the column through port 6 Figure 3 Valve in bypass needle in vial metering device aspirates sample volume 9LDO DVK SRUW 1HHGOH VHDW 6DPSOLQJ ORRS 6ROYHQW DVK SXPS 0HWHULQJ GHYLFH ...

Page 16: ...ow the needle The needle moves down into the vial the metering unit draws the required sample volume into the loop and the needle is raised In the next step the needle is washed see Figure 4 on page 16 Figure 4 Outer face of needle getting washed in wash port 9LDO DVK SRUW 1HHGOH VHDW 6DPSOLQJ ORRS 6ROYHQW DVK SXPS 0HWHULQJ GHYLFH ...

Page 17: ... is replaced in the sample tray The wash port flips into the origin position the needle is lowered into the needle seat and the injection valve switches back to mainpass position flushing the sample onto the column see Figure 5 on page 17 Figure 5 Valve switches to main pass sample is transferred towards the LC column 9LDO DVK SRUW 1HHGOH VHDW 6DPSOLQJ ORRS 6ROYHQW DVK SXPS 0HWHULQJ GHYLFH ...

Page 18: ...le Drive The needle movement is driven by a stepper motor connected to the spindle assembly by a toothed belt The circular motion of the motor is converted to linear motion by the drive nut on the spindle assembly The upper and lower needle positions are detected by reflection sensors on the needle station board while the needle in vial position is determined by counting the motor steps from the u...

Page 19: ... drive nut pushes the sapphire piston against the tension of the spring into the analytical head The base of the piston sits on the large bearing of the drive nut which ensures the piston is always centered A ceramic ring guides the movement of the piston in the analytical head The home position of the piston is sensed by an optical sensor on the hydraulic unit board while the sample volume is det...

Page 20: ...jection valve is driven by a stepper motor Only five of the six ports are used port 3 is not used A lever slider mechanism transfers the movement of the stepper motor to the injection valve Two microswitches monitor switching of the valve bypass and mainpass end positions No valve adjustments are required after replacing internal components ...

Page 21: ...ars The opening and closing of the gripper fingers are driven by a stepper motor linked by a toothed belt to the planetary gearing inside the gripper assembly The stepper motor positions are determined by the optical encoders mounted onto the stepper motor housing The encoders monitor the position of the motors continually and correct for position errors automatically e g if the gripper is acciden...

Page 22: ...n the sampler is hidden under the ICC Column Heater or Column Shelf respectively CAUTION Solvent incompatibility The solvent DMF dimethyl formamide leads to corrosion of the leak sensor The material of the leak sensor PVDF polyvinylidene fluoride is incompatible with DMF Do not use DMF NOTE The leak sensor in the sampler is hidden under the ICC Column Heater or Column Shelf respectively ...

Page 23: ...ction 1 Leak and Waste Handling Waste Concept 1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports 5043 1221 for optimal and safe waste disposal If you decide to use your own waste solution make sure that the tubes don t immerse in the liquid ...

Page 24: ...24 Agilent 1260 Infinity II Preparative Autosampler User Manual 1 Introduction Leak and Waste Handling ...

Page 25: ... Technologies 2 Site Requirements and Specifications Site Requirements 26 Physical Specifications 29 Performance Specifications 30 Specifications of the Sample Cooler 32 This chapter provides information on environmental requirements physical and performance specifications ...

Page 26: ...strumentation can result if the devices are connected to a line voltage higher than specified Connect your instrument to the specified line voltage only WARNING The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power Always u...

Page 27: ...ion can lead to electric shock or short circuit Never operate your instrumentation from a power outlet that has no ground connection WARNING Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment Never use a power cord other than the one that Agilent shipped with this instrument Never use the power cords that A...

Page 28: ...ondensation NOTE Agilent recommends that you install the HPLC instrument in the InfinityLab Flex Bench rack This option helps to save bench space as all modules can be placed into one single stack It also allows to easily relocate the instrument to another laboratory WARNING Heavy weight The module is heavy Carry the module at least with 2 people Avoid back strain or injury by following all precau...

Page 29: ...g temperature 4 40 C 39 104 F without chiller up to 55 C 131 F Ambient non operating temperature 40 70 C 40 158 F Humidity 95 r h at 40 C 104 F 1 1 If a sample cooler is included the upper value for humidity can be reduced Please check your lab conditions to stay beyond dew point values for non condensing operation Non condensing Operating altitude Up to 3000 m 9842 ft Non operating altitude Up to...

Page 30: ...ge 0 40 MPa 0 400 bar 0 5801 51 psi Sample viscosity range 0 2 5 cp Sample capacity 132 x 2 mL vial two trays default 100 x 2 mL vial two classic trays optional 36 x 6 mL vials two trays optional Carry over 0 005 50 ppm Method Column Agilent 5 Prep C18 21 2 mm x 50 mm 5 µm 446905 102 Eluent Water ACN 85 15 isocratic Flow rate 30 mL min Temperature RT Detection 273 4 nm Ref 360 100 nm 10 Hz Injecti...

Page 31: ...ntroller area network CAN Local Area Network LAN ERI ready start stop and shut down signals Safety and maintenance Extensive diagnostics error detection and display with Agilent Lab Advisor software Leak detection safe leak handling leak output signal for shutdown of pumping system and low voltages in major maintenance areas GLP features Early maintenance feedback EMF for continuous tracking of in...

Page 32: ...ial GWP see instrument label Table 3 Physical Specification of the Sample Cooler Type Specification Comments Weight 6 kg Dimensions height width depth 205 mm x 340 mm x 370 mm Refrigerant gas HFC 134a 0 042 kg Ozone depletion potential ODP 0 Supply voltage 24 VDC nominal Current 10 A max Ambient operating temperature 4 40 C 39 2 104 F Ambient non operating temperature 40 70 C 20 158 F Operating al...

Page 33: ... in accordance with national and local regulations Please contact your local Agilent Service Center in regard to safe environmental disposal of the appliance or check www agilent com for more info Table 4 Performance Specifications Agilent 1290 Sample Cooler Type Specifications Operating principle High performance low energy consumption micro compressor based cooler with ozone friendly HFC 134a co...

Page 34: ...34 Agilent 1260 Infinity II Preparative Autosampler User Manual 2 Site Requirements and Specifications Specifications of the Sample Cooler ...

Page 35: ... Module Magnets 36 Turn on off 37 Status Indicators 39 Exchange Drawers 40 Install the External Tray 42 Sample Trays 44 Choice of Vials and Caps 48 Transporting the Sampler with a Sample Cooler Installed 52 Agilent Local Control Modules 54 This chapter provides information on how to use the module ...

Page 36: ...36 Agilent 1260 Infinity II Preparative Autosampler User Manual 3 Using the Module Magnets Magnets 1 This stack exemplarily shows the magnets positions in the modules ...

Page 37: ...Agilent 1260 Infinity II Preparative Autosampler User Manual 37 Using the Module 3 Turn on off Turn on off 1 2 Power switch On 3 Turn instrument On Off with the control software 4 Power switch Off ...

Page 38: ...38 Agilent 1260 Infinity II Preparative Autosampler User Manual 3 Using the Module Turn on off 5 ...

Page 39: ...tatus indicators 1 Idle 2 Run mode 3 Not ready Waiting for a specific pre run condition to be reached or completed 4 Error mode interrupts the analysis and requires attention for example a leak or defective internal components 5 Resident mode blinking for example during update of main firmware 6 Bootloader mode fast blinking Try to re boot the module or try a cold start Then try a firmware update ...

Page 40: ... not operate the sampler without drawers installed NOTE Install all drawers for best cooling performance NOTE Do not mix standard and classic drawers Install classic drawer 1 50 to the left classic drawer 51 100 to the right side 1 Open the front door 2 Pull the drawer out 3 Lift the front of the drawer 4 Lift the drawer out ...

Page 41: ...ent 1260 Infinity II Preparative Autosampler User Manual 41 Using the Module 3 Exchange Drawers 5 Remove the drawer 6 Insert the back of the drawer 7 Align the drawer 8 Push in the drawer 9 Close the doors ...

Page 42: ...rnal Tray for 5 x 2 mL Vials G1313 27302 Disposal tube Preparations Turn the sampler off Open the doors Remove the samples NOTE Keep foam and plastic cover in a safe place For best temperature performance and if the external tray is not in use it is best to cover the opening for the external tray with the original parts 1 Lift the foam with a flathead screwdriver 2 Remove the foam ...

Page 43: ... II Preparative Autosampler User Manual 43 Using the Module 3 Install the External Tray 3 Push out the plastic cover Vent for the external tray is open 4 Insert the external tray 5 Install the disposal tube 6 Close the door ...

Page 44: ... is the usage of the classical drawer option 100 x 2 mL This option can t combine with the other drawers p n Description G7129 60010 Drawer for 66 x 2 mL Vials G7129 60110 Drawer for 18 x 6 mL Vials G7129 68210 Drawer Kit 100 x 2 mL Vials Classic G7129 60210 Drawer for 50 x 2 mL Vials Classic Left G7129 60220 Drawer for 50 x 2 mL Vials Classic Right G7129 60000 External Tray for 5 x 2 mL Vials NOT...

Page 45: ...to P1 F11 Left hand Drawer for 18 x 6 mL Vials P1 A1 to P1 C6 Right hand Drawer for 66 x 2 mL Vials P2 A1 to P2 F11 Right hand Drawer for 18 x 6 mL Vials P2 A1 to P2 C6 Drawer for 50 x 2 mL Vials Classic Left Vial 1 50 Drawer for 50 x 2 mL Vials Classic Right Vial 51 100 External Tray 5 position 201 205 Position The disposal tube is installed into the external tray by turning and pushing it into t...

Page 46: ...3 Using the Module Sample Trays Figure 11 Numbering of drawer position left hand Drawer for 18 x 6 mL Vials Figure 12 Numbering of drawer position right hand Drawer for 18 x 6 mL Vials Figure 13 Numbering of drawer position Drawer for 50 x 2 mL Vials Classic 3 3 3 3 ...

Page 47: ...Agilent 1260 Infinity II Preparative Autosampler User Manual 47 Using the Module 3 Sample Trays Figure 14 Numbering of tray position External tray DVWH LVSRVDO ...

Page 48: ... with the autosampler Crimp Top Vials p n Description 5181 3375 Crimp Top Vial 2 mL clear glass 100 Pack 5183 4491 Crimp Top Vial 2 mL clear glass 1000 Pack 5182 0543 Crimp Top Vial 2 mL clear glass write on spot 100 Pack 5183 4492 Crimp Top Vial 2 mL clear glass write on spot 1000 Pack 5183 4494 Crimp Top Vial 2 mL clear glass write on spot 100 Pack silanized 5181 3376 Crimp Top Vial 2 mL amber g...

Page 49: ...lass write on spot 100 Pack 5183 4506 Snap Top Vial 2 mL amber glass write on spot 1000 Pack 5183 4509 Snap Top Vial 2 mL amber glass write on spot 100 Pack silanized p n Description 5182 0714 Screw Cap Vials 2 mL clear glass 100 Pack 5183 2067 Screw Top Vial 2 mL clear glass 1000 Pack 5183 2070 Screw Top Vial 2 mL clear glass 100 Pack silanized 5182 0715 Screw Top Vial 2 mL clear glass write on s...

Page 50: ...blue aluminum septum clear PTFE red rubber 100 Pack 5181 1216 Crimp Cap green aluminum septum clear PTFE red rubber 100 Pack 5181 1217 Crimp Cap red aluminum septum clear PTFE red rubber 100 Pack p n Description 5182 0550 Snap Cap clear polypropylene septum clear PTFE red rubber 100 Pack 5182 3458 Snap Cap blue polypropylene septum clear PTFE red rubber 100 Pack 5182 3457 Snap Cap green polypropyl...

Page 51: ...d rubber 100 Pack 5182 0718 Screw Cap green polypropylene septum clear PTFE red rubber 100 Pack 5182 0719 Screw Cap red polypropylene septum clear PTFE red rubber 100 Pack 5182 0720 Screw Cap blue polypropylene septum clear PTFE silicone 100 Pack 5182 0721 Screw Cap green polypropylene septum clear PTFE silicone 100 Pack 5182 0722 Screw Cap red polypropylene septum clear PTFE silicone 100 Pack ...

Page 52: ... water inside the thermostat can safely flow into the leak funnel If condensate removal is done improperly you can harm the electronic of the module Otherwise no special precautions are needed for the modules WARNING Heavy weight The module is heavy Carry the module at least with 2 people Avoid back strain or injury by following all precautions for lifting heavy objects Ensure that the load is as ...

Page 53: ...on Also ensure there is no vial in the gripper arm Install the transport protection Remove all vials from the drawers The condensed water inside of the sample cooler is removed CAUTION Mechanical damage of the module If the transport assembly is not parked and not protected by the transport foam the module could be damaged due to excessive shock of the shipping container during transport Always pa...

Page 54: ...onitoring of Agilent Infinity II Prime LC Modules Excellent usability and ease of use through a user interface specifically tailored for mobile devices simple intuitive touch enabled and visual controllable High flexibility through a modern Bring your own device approach Connection between LC module and mobile device either wireless via WLAN or wired over USB cable with full package Convenient erg...

Page 55: ... 1200 Series 1200 Series SL and 1100 Series Excellent readability and usability by large colored display with background light high resolution and contrast Convenient ergonomic operation either handheld or at the stack with newly developed secure attachment Handheld or attached to a module in a stack to facilitate operator preferences The 1200 Infinity Series Instant Pilot provides Easy automation...

Page 56: ...56 Agilent 1260 Infinity II Preparative Autosampler User Manual 3 Using the Module Agilent Local Control Modules ...

Page 57: ... the Optional Sample Cooler Injector Step Commands 58 Damaged Packaging 59 Install the Sample Cooler 60 Using the Sample Cooler in an Infinity II Sampler 65 Control Interface 66 Control 67 Temperature Mode 68 Online Signal Monitor 69 This chapter describes the installation of the optional sample cooler ...

Page 58: ... sample Command also positions the vial at the seat and lifts the needle to the upper position Draw Metering device draws the defined injection volume Command also positions the vial at the seat lifts the needle and lowers the needle into vial Command can be done more than once maximum draw volume of 100 µL cannot be exceeded Use Plunger Home to reset the metering device Needle Up Lifts the needle...

Page 59: ...tely Inform your service representative that the instrument may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged Notify your Agilent sales and service office about the damage An Agilent service representative...

Page 60: ...of the module Before dismounting the sample cooler For new installation wait at least 30 min before switching on the compressor of the sample cooler Make sure the power cords are disconnected Make sure there is no condensate inside the module NOTE If the sample cooler is disconnected from the power supply you should wait for at least five minutes before replugging and switching on the compressor a...

Page 61: ...the serial number of the sample cooler must be DEBAT02001 or higher NOTE For optimal cooling performance of the autosampler all drawers have to be installed 1 Ensure that the power switch on the front of the module is OFF switch stands out 2 Ensure that the power cable is removed from the instrument 3 Open the 4 screws on the rear of the module 4 Remove the sample cooler mainframe cover ...

Page 62: ...ug Do not use the sample cooler if it is not operating correctly or has been damaged Disconnect it from the power supply and call your local service center Remove the power cable from the module before opening the cover Do not connect the power cable to the module while the covers are removed If the sample cooler is disconnected from the power supply you should wait for at least five minutes befor...

Page 63: ...oler CAUTION Damage to the cables Do not bend or pinch the cables Fit in the sample cooler perfectly 7 Slide in the whole unit 8 Tighten the 4 screws which holds the sample cooler unit in place 9 Use a bubble level to check the leveling of the sampler NOTE The sample cooler should be operated in a proper horizontal position 10 PP ...

Page 64: ... Cooler 11 12 NOTE Check leak waste handling for further info CAUTION Damage to the sample cooler Wait at least 30 min before switching on the compressor of the sample cooler This allows the refrigerant and system lubrication to reach equilibrium 13 Connect the power cable to the power connector at the rear of the module P P PP ...

Page 65: ... loading the Agilent CDS the module should appear as an active item in the graphical user interface GUI Within the autosampler GUI there are active areas If the mouse cursor is moved across the icons the cursor will change see the following figure ALS configuration is displayed when moving with the mouse cursor over the syringe The information provides ALS related information like Part number ALS ...

Page 66: ...Preparative Autosampler User Manual 4 Installation of the Optional Sample Cooler Using the Sample Cooler in an Infinity II Sampler Control Interface A right click into the active area will open a menu to show the Control Interface ...

Page 67: ...e Cooler section of the Control dialog box is available when you have a cooler installed It allows you to switch the cooler on and off manually Select On to switch on the cooler Specify the required temperature in the adjacent field Note that the specified temperature must be at least 5 C below ambient for proper temperature control Select Off to switch off the cooler ...

Page 68: ... current method The sample temperature can not be directly controlled only the temperature of the air stream from the thermostat If the air stream has the correct temperature during method execution it may have been stored at higher temperatures with previous methods As a result we are now introducing two modes which can be configured in the dashboard Constant temperature mode this mode uses the c...

Page 69: ...the Sample Cooler in an Infinity II Sampler Online Signal Monitor The online signal monitor for the chiller is also available and allows you to monitor the WPS1A temperature signals The Online Plot Window should look like this If the change button is pressed you can select the Chiller signal WPS1A in the online plot ...

Page 70: ...he doors are opened frequently warm and or humid air enters the workspace of the autosampler Try to limit opening the door drawers in extremely hot or humid weather Temperature rise Due to the warm air temperatures generated during heating from a cold to a warm chiller set point it is possible to see moisture forming on the shelves and the vials This moisture is caused by the warm air coming into ...

Page 71: ...off a Remove all sample containers or vials from the autosampler b Let the autosampler temperature stabilize to ambient temperature open the door far enough for air to get in c Clean the drawers of the sample hotel or the cold reservoir underneath the drawers in the sampler wipe them down and dry them well Drawer Alarm only for the Multisampler The Drawer Alarm feature sounds an alarm when the sam...

Page 72: ...72 Agilent 1260 Infinity II Preparative Autosampler User Manual 4 Installation of the Optional Sample Cooler Using the Sample Cooler in an Infinity II Sampler ...

Page 73: ...dling 74 Preparing the Module 75 Solvent Information 76 Capillary Color Coding Guide 82 Swage Fittings 83 Flow Connections to the sampler 85 Setting up the Sampler 87 Control Settings 90 Method Parameter Settings 91 Injector Programm 95 Module Configuration View 96 This chapter explains the operational parameters of the module ...

Page 74: ...erature in the sample compartment at least 25 K below the boiling point of the solvent used Do not operate the instrument in an explosive atmosphere Do not use solvents of ignition Class IIC according IEC 60079 20 1 for example carbon disulfide Reduce the volume of substances to the minimum required for the analysis Never exceed the maximum permissible volume of solvents 8 L in the solvent cabinet...

Page 75: ...of solvents see Solvent Information on page 76 Priming and Purging the System When the solvents have been exchanged or the system has been turned off for a certain time for example overnight oxygen will re diffuse into the solvent channel Therefore priming and purging of the system is required before starting an application Table 6 Choice of Priming Solvents for Different Purposes Activity Solvent...

Page 76: ...nt materials like flow cells valve materials etc and recommendations in subsequent sections Recommended Wash Solvents water ethanol methanol water acid especially for basic compounds water base especially for acidic compounds water acetonitrile Material Information Materials in the flow path are carefully selected based on Agilent s experiences in developing highest quality instruments for HPLC an...

Page 77: ...ical compatibility of materials PEEK PEEK Polyether Ether Ketones combines excellent properties regarding biocompatibility chemical resistance mechanical and thermal stability PEEK is therefore the material of choice for UHPLC and biochemical instrumentation It is stable in the specified pH range for the Bio inert LC system pH 1 13 see bio inert module manuals for details and inert to many common ...

Page 78: ...ability for most common inorganic solvents including acids and bases in a pH range of 1 to 12 5 It is compatible to many organic solvents used in chromatographic systems like methanol acetonitrile and isopropanol It has limited stability with aliphatic aromatic and halogenated hydrocarbons THF phenol and derivatives concentrated acids and bases For normal phase applications the maximum pressure sh...

Page 79: ...tic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF Titanium Ti Titanium is highly resistant to oxidizing acids for example nitric perchloric and hypochlorous acid over a wide range of concentrations and temperatures This is due to a thin oxide layer on the s...

Page 80: ... of flow cells with sapphire windows is recommended Gold Gold is inert to all common HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia Zirconium Oxide ZrO2 Zirconium Oxide is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Platinum Iridium P...

Page 81: ... with HFIP it is best to dedicate a particular chamber to this solvent not to switch solvents and not to let dry out the chamber For optimizing the life of the pressure sensor do not leave HFIP in the chamber when the unit is off The tubing of the leak sensor is made of PVDF polyvinylidene fluoride which is incompatible to the solvent DMF dimethyl formamide Sapphire Ruby and Al2O3 based ceramics S...

Page 82: ...82 Agilent 1260 Infinity II Preparative Autosampler User Manual 5 Preparing the Module Capillary Color Coding Guide Capillary Color Coding Guide Figure 15 Syntax for capillary description ...

Page 83: ...t over the end of the tubing 3 Carefully slide the ferrule components on after the nut and then finger tighten the assembly while ensuring that the tubing is completely seated in the bottom of the end fitting 4 Use a column or injection valve to gently tighten the fitting which forces the ferrule to seat onto the tubing NOTE Don t overtighten Overtightening will shorten the lifetime of the fitting...

Page 84: ...ttings Figure 16 Examples of incorrect fittings 0L LQJ FKDPEH OHDNV ZLOO RFFXU HUUXOH FDQQRW VHDW SURSHUO I GLPHQVLRQ LV WRR ORQJ I GLPHQVLRQ LV WRR VK D GHDG YROXPH RU PL LQJ FKDPEHU ZLOO RF NOTE The first time that the swagelock fitting is used on a column or an injection valve the position of the ferrule is permanently set If changing from a column or an injection valve to another the fitting m...

Page 85: ...nect it to the peristaltic pump 4 Route the drainage of the washport outlet to the waste container 5 Prime the wash tubing 6 Install the capillary from the pump outlet into the port 1 of the injection valve Preparations Module is installed in the system Use an appropriate solvent based on the sample and mobile phase chemistries The composition of the wash solvent should be the most solubilizing co...

Page 86: ...5 Preparing the Module Flow Connections to the sampler 7 Install the capillary from port 6 of the injection valve to the ICC or MCT The correct plumbing is shown in the figure below and is attached to the module 8 Prime and purge the complete sampler DVWH ROXPQ 3XPS ...

Page 87: ... on Injection volume Sample location A right click into the Active Area will open a menu with the option to adjust the following parameters Show the Control User Interface special module settings See Control Settings on page 90 Show the Method User interface same as via menu Instrument Set up Instrument Method Setup G7129B See Method Parameter Settings on page 91 Injector Program When you activate...

Page 88: ... Error text Error Red Not ready yellow Ready green Pre run Post run purple Run blue Idle green Offline dark gray Standby light gray EMF Status shows Run Ready Error state and Not Ready text or Error text Offline gray Ok No Maintenance required green EMF warning Maintenance might be required yellow EMF warning Maintenance required red Table 7 The Vialsampler User Interface ...

Page 89: ...Agilent 1260 Infinity II Preparative Autosampler User Manual 89 Preparing the Module 5 Setting up the Sampler Figure 17 ChemStation Method and Run Control ...

Page 90: ...igured Mark the Turn on Cooler check box to specify that the cooler is switched on automatically when the instrument is switched on Cooler The section is available when a cooler is installed and configured and the Constant temperature mode is selected Select On to switch on the cooler Specify the required temperature in the adjacent field The specified temperature must be at least 5 C below ambien...

Page 91: ...osampler User Manual 91 Preparing the Module 5 Setting up the Sampler Method Parameter Settings The method parameter settings are available via right click on the active area of the grafical user interface Figure 19 Method parameter settings ...

Page 92: ...is enabled only if you have selected Flush Port Specify a flush time in seconds between 1 and 100 in steps of 1 Location enabled only if you have selected a Wash Vial Allows you to specify a wash location in the format Vial X where X is the vial number Specify well locations in the format P Plate Row Character Column Number for example P1 A1 The values of Row Character and Column Number depend on ...

Page 93: ...ed for an injection cycle For viscous samples in combination with multidraw use a slow Eject Speed Wait Time After Draw ensures that the temporary vacuum which originates from the drawing of liquid from the sample vial dissipates The needle stays first on the seat for the specified time then after drawing sample from the vial remains there for the specified time Needle Height Offset a vertical off...

Page 94: ...obtain the desired degree of flushing to prevent sample carry over Overlapped Injection Mode provides faster throughput of samples by allowing the preparation of the next injection while the current injection is being completed The following options can be selected from the drop down list Off to switch off overlapped injection Select When sample is Flushed Out to specify that the sample can be pre...

Page 95: ...njector program comprises a series of numbered lines each specifying an operation that the autosampler carries out sequentially When you activate a pretreatment injector program it replaces the standard injection cycle The following functions are available Draw Eject Mix Inject Move Wait Valve Needle Wash Remote Wait For Repeat End Repeat Comment ...

Page 96: ...ngs are available via menu Instrument Show ConfigUI Figure 20 Configuration view NOTE For additional help and support Highlight the desired area and press the F1 key A help screen will open with additional information and documentation about the topic NOTE The vialsampler configuration is done in the module dashboard context menu not in the instrument configuration ...

Page 97: ...e Wash Wash Port 98 Using an Injector Program 100 General Recommendation to Lowest Carry Over 101 Fast Injection Cycle and Low Delay Volume 102 Overlapped Injection Mode 102 General Recommendations for Fast Injection Cycle Times 103 Precise Injection Volume 104 Draw and Eject Speed 104 Choice of Rotor Seal 106 This chapter provides information on how to optimize the module ...

Page 98: ...centration carry over may become noticeable Using the automated needle wash enables the carry over to be minimized and prevents also contamination of the needle seat Using the Automated Needle Wash Wash Port The automated needle wash can be programmed either as method parameter enable needle wash or the needle wash can be included into the injector program When the automated needle wash is used th...

Page 99: ...an use an injector program and an additional wash vial with an appropriate and stronger solvent for cleaning The wash vial should contain solvent in which the sample components are soluble and the vial should not be capped If the wash vial is capped small amounts of sample remain on the surface of the septum which may be carried on the needle to the next sample Injector Program with Needle Wash Th...

Page 100: ...libration time to ensure that the bypass grove is filled with the start concentration of the mobile phase Otherwise the separation could be influenced especially if microbore columns are used Example Outside wash of needle in vial p2 A1 before injection Injector program Draw x x y μl from sample WASH vial location p2 A1 Inject Wait equilibration time see text above Valve bypass Wait 0 2 min Valve ...

Page 101: ...owing procedure can be used to clean the needle seat In LabAdvisor go to Maintenance Change Needle this will set the needle into home position Remove the safety cover of the sampler Be carefully not to risk an injury by the uncovered needle Pipette an appropriate solvent on to the needle seat The solvent should be able to dissolve the contamination If the contamination is unknown use 2 or 3 solven...

Page 102: ...njection cycle starts but waits with switching to mainpass until the actual run is finished You gain the sample preparation time when using this process Switching the valve into the bypass position reduces the system delay volume the mobile phase is directed to the column without passing sample loop needle and needle seat capillary This can help to have faster cycle times especially if low flow ra...

Page 103: ...es Add at last run a blank if overlapped injection is used To reduce the injection time the detector balance has to be set to OFF NOTE For the last injection of the sequence with overlapped injections it has to be considered that for this run the injection valve is not switched as for the previous runs and consequently the injector delay volume is not bypassed This means the retention times are pr...

Page 104: ...injection volumes 2 μL If the pump has no degasser it is recommended to use an Agilent 1260 Infinity degasser Also using the automated needle wash see Optimization for Lowest Carry over on page 98 between injections reduces carry over to a minimum further improving the injection volume precision Draw and Eject Speed Draw Speed The speed at which the metering unit draws sample out of the vial may h...

Page 105: ... value speeds up the injection cycle by shortening the time the metering unit requires to eject solvent at the beginning of the injection cycle when the piston returns to the home position An EJECT statement in an injector program also uses the eject speed setting which is configured for the autosampler A faster eject speed shortens the time required to run the injector program When using viscous ...

Page 106: ...table for the majority of applications However for applications using mobile phases with pH below 2 3 or above 9 5 the Vespel seal may degrade faster leading to reduced seal lifetime PEEK Seal for 1260 injection valve only The 1260 injection valve has a sealing material made of PEEK This material has high chemical resistance and versatility It is suitable for application using mobile phases within...

Page 107: ...ge Metering Seal or Piston 113 Park Arm 114 Change Gripper 116 Automatic Referencing 117 Sampler Gripper Verification Test 118 Step Commands 119 Tests 120 Sample Cooler Function Test 120 Troubleshooting 121 Troubleshooting Guide for the Sample Transport Assembly 122 Intermittent lock ups with or without vial in the gripper fingers 123 Poor alignment 124 Agilent Lab Advisor Software 125 Overview ab...

Page 108: ...lic failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Maintenance Functions The maintenance functions position the needle arm gripper assembly and metering device for easy access when doing maint...

Page 109: ...the Module s Indicators and Test Functions Step Commands The step functions provide the possibility to execute each step of the sampling sequence individually The step functions are used primarily for troubleshooting and for verification of correct autosampler operation after repair see Step Commands on page 119 ...

Page 110: ...ance positions can be selected from the Service Diagnostic Maintenance Positions menu in the Diagnosis display User Interface The functions for the control software are Change Needle Sample Loop or Needle Seat The needle moves slowly down and positions the needle arm for easy access to the needle the sample loop or the needle seat Change Metering Seal or Piston relieves the tension on the metering...

Page 111: ...igin position Next The needle arm moves in the desired position and guide the user to the next procedure step Move Down The needle arm moves slowly down Move Up The needle arm slowly moves up WARNING For the needle exchange procedure the needle arm moves slowly down while the safety cover is removed Risk of personal injury due to moving needle Keep fingers off the moving needle Wear safety goggles...

Page 112: ...bly first Then replace the needle seat assembly After the exchanging of the needle seat reinstall the new needle assembly See Exchange the Needle Assembly on page 154 and Exchange the Needle Seat Assembly on page 159 5 Next positions the needle to the top level Flap the safety wash port back in its origin position 6 Reinstall the safety cover and complete the procedure 7 Select Next to move the ne...

Page 113: ... for the control software are Start Draws the piston away from the home position relieving the tension on the spring Next Repositions the piston at the home position Back Completes the procedure Using the Change Seal Function 1 Select Start to move the piston to the maintenance position 2 Exchange the metering seal see Exchange the Metering Seal and Piston on page 173 3 Select Next to move the pis...

Page 114: ...Interface In the control software the Park Arm command is part of the ALS positions that can be selected from the special commands menu in the Instrument Control display The commands for the control software are Park Arm moves the gripper arm to the park position Home moves the gripper arm out of the park position to the home position ...

Page 115: ...e preparation 1 Select Park Arm 2 When the arm is in the park position install Transport Protection G7129 40050 Then the autosampler is ready for shipment and can be switched OFF When Before transporting or shipping the module CAUTION Unsecured Transportation of the module Unsecured transportation of the module may result in mechanical damage to the gripper and transport slider Always secure the a...

Page 116: ...the transport assembly and gripper arm to the position required to change the gripper arm Next Repositions the transport assembly and gripper arm to the home position Back Completes the procedure Using the Change Gripper Function 1 Select Start to move the gripper arm to the maintenance position 2 Exchange the gripper arm see Exchange the Gripper Arm on page 177 3 Select Next to move the gripper a...

Page 117: ...ral tray positions as reference points Because the tray is a rectangle a four point alignment is sufficient to correct all other vial positions within the tray On completion of the procedure the corrected gripper positions are stored in the instrument firmware Start Moves the transport assembly and gripper arm to the position required to install the calibration tools Next Mount the calibration fix...

Page 118: ...erification Description The verification procedure uses several vial positions as reference points to verify the gripper alignment is correct If verification indicates one or more positions are out of alignment the Automatic Referencing procedure should be done Allows you to select a vial position You can select from positions 1 11 39 56 66 101 111 139 156 and 166 ...

Page 119: ... to bypass if it is not already in that position Vial to Seat Moves the selected vial to the seat position Command also lifts the needle to the upper position Needle into Sample Lowers the needle into the sample Command also positions the vial at the seat and lifts the needle to the upper position Draw Metering device draws the defined injection volume Command also positions the vial at the seat l...

Page 120: ...n that the sample cooler is functioning After the test has been started it begins to acquire data from the cooler s PT1000 temperature sensor As soon as the temperature has equilibrated that is the temperature does not change by more than 0 5 Cover a 10 s period the cooler is turned on and measurement is started For the test to succeed three temperature checkpoints must be reached within a specifi...

Page 121: ...ampler is unable to perform a specific step due to a hardware failure an error message is generated You can use the injector steps to do the injection sequence while observing how the instrument responds Automatic Referencing on page 117 summarizes the injector steps and lists the associated error messages and probable causes of step failures ...

Page 122: ...h or without vial in the gripper fingers with error messages Many times the sampler is being used very heavily motor overtemp 0 or 1 or 2 or 3 movement failed 0 or 1 or 2 or 3 missing vial 2 Jittery shaky movement in X and or theta axes and or when the needle goes through the gripper arm into the vial with error messages motor overtemp 0 or 2 movement failed 0 or 2 3 Poor alignment seen during via...

Page 123: ...0 see Exchange the Gripper Arm on page 177 4 Start automatic referencing in Lab Advisor For this procedure it is mandatory to use the calibration tool On completion of the procedure the corrected values are stored in the instrument firmware 5 Exchange Transport assembly Sample Transport Assembly G7129 60600 6 Exchange the Autosampler Main Board Autosampler Specific Board G7129 65800 or the fusion ...

Page 124: ...ly G1313 60010 see Exchange the Gripper Arm on page 177 3 Exchange Transport assembly Sample Transport Assembly G7129 60600 4 Exchange the Autosampler Main Board Autosampler Specific Board G7129 65800 or the fusion board Fusion Board G4200 65880 5 For all three steps step 2 3 and 4 you have to do an automatic referencing again to store the correct values in the instrument firmware again WARNING Pe...

Page 125: ...every Agilent 1200 Infinity Series and Agilent InfinityLab LC Series instrument The Lab Advisor Advanced features can be unlocked by purchasing a license key and include real time monitoring of instrument actuals all various instrument signals and state machines In addition all diagnostic test results calibration results and acquired signal data can be uploaded to a shared network folder The Revie...

Page 126: ...126 Agilent 1260 Infinity II Preparative Autosampler User Manual 7 Troubleshooting and Diagnostics Agilent Lab Advisor Software ...

Page 127: ...r 132 Leak Sensor Open 133 Leak Sensor Short 134 Compensation Sensor Open 134 Compensation Sensor Short 135 Fan Failed 135 Sampler Error Messages 136 Defective Gripper Assembly 136 Valve Failed 136 Needle Movement Failed 137 Missing Vial 137 Initialization Failed 138 Metering Home Failed 139 Motor Temperature 140 Initialization with Vial 141 Vial in Gripper 141 Missing Wash Vial 142 Invalid Vial P...

Page 128: ...essages 144 Cooler Voltage Failure 144 Cooler PCB Error State 144 Cooler temperature below or above target temperatur 145 Cooler Overpressure Failure 145 Cooler Condensate Error 146 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions ...

Page 129: ...et a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started if the error has been resolved for example liquid from a leak has bee...

Page 130: ... not ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required Probable cause Suggested actions 1 Leak detected in another module with a CAN connection to the system Fix the leak in the external instrument before restarting the mo...

Page 131: ...ystem configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 Not ready condition in one of the instruments connected to the remote line Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis 2 Defective remote cable Exchange the ...

Page 132: ...nt This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 3 Leaking rotor seal or needle seat Exchange the rotor seal or seat capillary 4 Defective metering seal Exchange the metering seal Make sure the leak sensor is...

Page 133: ...he leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the Power Switch board Ensure the leak sensor is connected correctly 2 Defective leak sensor Exchange the leak sensor 3 Leak sensor incorrectly routed being pinched by a metal compo...

Page 134: ... NTC on the power switch board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Exchange the leak sensor 2 Leak sensor incorrectly routed being pinched by a m...

Page 135: ... The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated This limit is given by 2 revolutions second for longer than 5 seconds Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable c...

Page 136: ...ls to reach the mainpass position or if the microswitch does not close the error message is generated Probable cause Suggested actions 1 Mechanical obstruction Ensure unobstructed movement of the transport assembly 2 High friction in the transport assembly Exchange the sample transport assembly 3 Defective motor assembly Exchange the sample transport assembly 4 Defective sample transport assembly ...

Page 137: ...onitors the gripper motor encoder If a vial is present the closing of the gripper fingers is limited by the vial However if no vial is present the gripper fingers close too far This is sensed by the processor encoder position causing the error message to be generated Probable cause Suggested actions 1 Defective or dirty position sensor Exchange the needle station board 2 Defective motor Exchange t...

Page 138: ...rocessor monitors the position sensors and motor encoders to check for correct movement If one or more of the movements is not successful or is not detected the error message is generated Probable cause Suggested actions 1 Mechanical obstruction Ensure unobstructed movement of the transport assembly 2 Defective needle station board Exchange and reset the needle station board 3 Defective transport ...

Page 139: ...f the piston If the piston fails to move to the home position or if the sensor fails to recognize the piston position the error message is generated Probable cause Suggested actions 1 Blockage in the flow path Change needle or needle seat 2 Defective sensor Exchange the optical sensor cable 3 Broken piston Exchange the metering piston and seal 4 Defective metering drive motor Exchange the metering...

Page 140: ...ers The processor monitors the current drawn by each motor and the time the motor is drawing current The current drawn by the motors is dependent on the load on each motor friction mass of components etc If the current drawn is too high or the time the motor draws current is too long the error message is generated Probable cause Suggested actions 1 Mechanical obstruction Ensure unobstructed moveme...

Page 141: ...ted Vial in Gripper Error ID 34308 The gripper arm attempted to move with a vial still in the gripper During specific stages of the sampling sequence no vial should be held by the gripper The autosampler checks if a sample vial is stuck in the gripper by closing and opening the gripper fingers while monitoring the motor encoder If the gripper fingers are unable to close the error message is genera...

Page 142: ...transport assembly flex board are used to automatically check which sample trays are installed coding on tray If the vial position does not exist in the current sample tray configuration the error message is generated Probable cause Suggested actions 1 No wash vial in the position defined in the method Install the wash vial in the correct position or edit the method accordingly Probable cause Sugg...

Page 143: ...nto the position The valve position sensor monitors the position of the valve If the valve fails to switch into the position mainpass or bypass or if the sensor fails to recognize the valve position the error message is generated Probable cause Suggested actions 1 Defective rotor seal stator head Exchange the rotor seal stator head 2 Defective injection valve Exchange the injection valve ...

Page 144: ...efective Probable cause Suggested actions 1 Blown fuses defective cable Check the power cable from the ALS to the sample cooler Check if a fuse is blown LED status on the specific mainboard 2 Voltage issue in the sample cooler Replace the sample cooler first then check the specific mainboard Probable cause Suggested actions 1 Blown fuses defective cable Check the power cable from the ALS to the sa...

Page 145: ... sample cooler pressure sensor has detected an overpressure Probable cause Suggested actions 1 Condensate inside of the chiller Check if the drainage is ok 2 Defective cable Check the signal cable from the ALS to the sample cooler 3 Defective temperature sensor Replace the sample cooler Probable cause Suggested actions 1 Overpressure in the chiller Switch off the instrument and wait for 15 min Res...

Page 146: ... actions 1 Blocked drainage Switch off the instrument and remove the condensate tubings Place a beaker underneath the waste outlet Check the waste container There should be no kinked and sealed tubing For further information see Install the Sample Cooler on page 60 2 Defective condensate sensor Switch the sample cooler off on and check if after 8 min the error shows up again Replace the condensate...

Page 147: ...e 152 Remove and Install Doors 153 Exchange the Needle Assembly 154 Exchange the Needle Seat Assembly 159 Exchange the Sample Loop Assembly 164 Exchange the Rotor Seal 167 Exchange the Metering Seal and Piston 173 Exchange the Gripper Arm 177 Replace the Peristaltic Pump Cartridge 179 Exchange the Wash Port Assembly 182 Replace the Module Firmware 187 This chapter describes the maintenance of the ...

Page 148: ...enance Introduction to Maintenance The module is designed for easy maintenance Maintenance can be done from the front with module in place in the system stack Status indicator Needle assembly Drawer Column shelf Leak drain Power switch NOTE There are no serviceable parts inside Do not open the module ...

Page 149: ... product user guides or use of the products in violation of applicable laws rules or regulations Use your Agilent products only in the manner described in the Agilent product user guides WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened Do not remove the cover of the module Only certified persons are authorized to carry out r...

Page 150: ...good laboratory practice The volume of substances should be reduced to the minimum required for the analysis Do not operate the instrument in an explosive atmosphere CAUTION Safety standards for external equipment If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external...

Page 151: ...rvice representatives on a contractual basis For information regarding the maintenance inspection contract contact your Agilent representative The following pages describe the maintenance simple repairs of the module that can be carried out without opening the main cover Table 11 Overview of maintenance Procedure Typical interval minimum Notes Change needle needle seat 30000 needle into seat movem...

Page 152: ...case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module Do not use an excessively damp cloth during cleaning Drain all solvent lines before opening any connections in the flow path ...

Page 153: ...ired Description Flat screwdriver Parts required p n Description G7129 68702 Door Assy Preparations Finish any pending acquisition job and return any plate on the workspace back to the hotel 1 Press the release buttons and pull the front door out 2 For the Installation of the front door insert the hinges into their guides and move the door in until the release buttons click into their final positi...

Page 154: ... shutoff valves WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow g...

Page 155: ...re select Service Diagnostics in the system screen Tools Maintenance Positions Change Needle Loop click Start and wait until the needle assembly is in maintenance position 2 Open the front door 3 Unscrew the safety cover screw WARNING Sharp needle Uncovered needles may cause injuries Do not touch the tip of the needle 4 Remove the safety cover ...

Page 156: ...use injuries Do not change the needle seat at this point Do not touch the tip of the needle 5 Turn or flap the wash port to the left 6 In Lab Advisor use Next to move the needle in the down position The needle will move slowly downwards and will stop approximately 2 mm above the seat 7 Disconnect the sample loop from the needle 8 Loosen the fixing screw ...

Page 157: ...n the needle in the seat then tighten the screw firmly 2 11 Align the needle in the seat needle may be bent slightly manually if not aligned properly 12 Reconnect the sample loop to the needle NOTE Do not overtighten the fitting NOTE Incorrect positioning and installation of the needle loop connection can result in damaging and breaking the sample loop 1 2 ...

Page 158: ...13 In Lab Advisor use Next to lift the needle slowly into the up position 14 Turn or flap the wash port to the right 15 Install the safety cover 1 and fix the screw 2 16 Select Next to move the needle into the needle seat 17 Select Back to finish the procedure 18 Close the doors 19 Perform a pressure test 1 2 ...

Page 159: ...uisition job and in order to avoid leaks stop the pump running and remove the tubings from the solvent bottles If available close the shutoff valves WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator Do not open the safety cover of the needle station during normal operation Wear safety goggles and safety gloves when removing the needle assembly 1 In th...

Page 160: ...njuries Do not touch the tip of the needle 4 Remove the safety cover WARNING Sharp needle Uncovered needles may cause injuries Do not change the needle seat at this point Do not touch the tip of the needle 5 Turn or flap the wash port to the left 6 In Lab Advisor use Next to move the needle in the down position The needle will move slowly downwards and will stop approximately 2 mm above the seat ...

Page 161: ...mbly 7 Disconnect the sample loop from the needle 8 Loosen the fixing screw 9 Lift out the needle 10 Disconnect the seat capillary fitting from the injection valve port 5 11 Use a small flat head screwdriver to ease out the needle seat 12 Insert the new needle seat assembly Press the seat firmly into position ...

Page 162: ...ion valve 14 Insert the needle 1 Align the needle in the seat then tighten the screw firmly 2 15 Reconnect the sample loop to the needle NOTE Do not overtighten the fitting NOTE Incorrect positioning and installation of the needle loop connection can result in damaging and breaking the sample loop 16 In Lab Advisor use Next to lift the needle slowly into the up position 1 2 ...

Page 163: ...ce 9 Exchange the Needle Seat Assembly 17 Turn or flap the wash port to the right 18 Install the safety cover 1 and fix the screw 2 19 Select Next to move the needle into the needle seat 20 Select Back to finish the procedure 21 Close the doors 22 Perform a pressure test 1 2 ...

Page 164: ...g and remove the tubings from the solvent bottles If available close the shutoff valves WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator Do not open the safety cover of the needle station during normal operation Wear safety goggles and safety gloves when removing the needle assembly 1 In the Instant Pilot start the maintenance mode and select Change ...

Page 165: ...mple Loop Assembly 3 Unscrew the safety cover screw WARNING Sharp needle Uncovered needles may cause injuries Do not touch the tip of the needle 4 Remove the safety cover 5 Check if the needle is in maintenance position 6 Disconnect the loop capillary from the metering device and from the needle ...

Page 166: ... needle loop connection can result in damaging and breaking the sample loop 8 In Lab Advisor use Next to lift the needle slowly into the up position 9 Turn or flap the wash port to the right 10 Install the safety cover 1 and fix the screw 2 11 Click Next until the needle assembly is moved to the needle seat 12 Select Back to finish the procedure 13 Close the doors 14 Perform a pressure test 1 2 ...

Page 167: ...equired p n Description 5068 0268 Rotor Seal 5068 0267 Stator Preparations Finish any pending acquisition job and in order to avoid leaks stop the pump running CAUTION Reduced life time of the injection valve Component cleanliness is crucial for the life time of the injection valve Replace the rotor seal in a clean environment CAUTION Removing the stator head The stator face is held in place by th...

Page 168: ... Manual 9 Maintenance Exchange the Rotor Seal 1 Open the front door 2 Remove all capillaries from the injection valve with a 1 4 inch wrench 3 Loosen each fixing bolt two turns at a time Remove the bolts from the head 4 Remove the stator head and stator ring ...

Page 169: ...ith the appropriate solvent If more stringent cleaning is required use a sonicator Inspect the remaining valve components for contamination Clean them as necessary If the stator head is scratched replace the valve 6 Install the new rotor seal and isolation seal Ensure the metal spring inside the isolation seal faces towards the valve body NOTE Make sure that the rotor sealing surface with its engr...

Page 170: ...the 12 o clock position Ensure the ring sits flat on the valve body 8 Install the stator head Tighten the bolts alternately two turns at a time until the stator head is secure NOTE Do not over tighten the screws The screws hold the assembly together and do not affect the sealing force The sealing force is automatically set as the screws close the stator head against the valve body ...

Page 171: ...anual 171 Maintenance 9 Exchange the Rotor Seal 9 Reconnect the capillaries and tubes to the valve ports The correct plumbing is shown below and on the safety cover of the module 10 Slide the waste tube into the waste holder in the leak tray DVWH ROXPQ 3XPS ...

Page 172: ...172 Agilent 1260 Infinity II Preparative Autosampler User Manual 9 Maintenance Exchange the Rotor Seal 11 Close the doors 12 Perform a pressure test ...

Page 173: ... 3 mm12 cm long 01018 23702 Insert tool Cleaning tissue and appropriate solvent like isopropanol or methanol Parts required p n Description 0905 1294 Metering seal 900 µL G4267 60462 Piston Assembly 900 µL Preparations Finish any pending acquisition job and in order to avoid leaks stop the pump running 1 In the Tools section of the Agilent Lab Advisor software select Service Diagnostics Maintenanc...

Page 174: ...on 3 Disconnect the two capillaries from the metering device 4 Remove the two fixing bolts 5 Pull the metering head assembly away from the sampler 6 Remove the two fixing bolts from the base of the metering head assembly 7 Disassemble the metering head assembly 8 Use a seal insert tool to carefully remove the seal ...

Page 175: ...Reassemble the metering head assembly Carefully insert the piston into the base The closed side of the metering head must be on the same side as the lower one of the two capillary drillings 13 Install the fixing bolts Tighten the bolts securely Tighten the bolts alternately two turns at a time until the stator head is secure The housing must flush with the metering head 14 Install the metering hea...

Page 176: ... Reinstall the capillaries 16 In the Tools section of the Agilent Lab Advisor software system screen exit Service Diagnostics Maintenance Positions Change Metering Device click Next and wait until the metering device is in Home position 17 Select Back to finish the procedure 18 Close the doors 19 Perform a pressure test ...

Page 177: ...quisition job 1 In the Local Controller start the maintenance mode and select Change Gripper function OR In the Tools section of the Agilent Lab Advisor software select Service Diagnostics Maintenance Positions Change Gripper click Start and wait until the gripper is in maintenance position 2 Open the front door 3 Identify the slit below the gripper motor and the gripper arm release button 4 Rotat...

Page 178: ...aper clip in place press the gripper release button and rotate the gripper arm to the right 7 The gripper arm will come off 8 Replace the gripper arm by holding the paper clip in place pushing the gripper arm into the holder and rotating the gripper arm to the left 9 In the Local Controller close Change gripper OR In the Agilent Lab Advisor software select Change gripper Next click Next and wait u...

Page 179: ... the solvent to the peristaltic pump to avoid syphoning effects WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent...

Page 180: ...eristaltic pump cartridge 3 Pull the cartridge forward off the motor shaft 4 Disconnect the tubing coupler leading to the wash port and the tubing coupler coming from the solvent bottle 5 Push the new cartridge onto the motor shaft until the clips click into place 6 Connect the wash port tubing to the upper tubing of the new cartridge use sand paper to get a good grip on the tubing ...

Page 181: ...81 Maintenance 9 Replace the Peristaltic Pump Cartridge 7 Connect the inlet filter of the solvent bottle Use the syringe to draw enough solvent for completely filling of the peristaltic pump tubing before continuing to prime the peristaltic pump 8 Close the doors ...

Page 182: ...void leaks stop the pump running and remove the tubings from the solvent bottles If available close the shutoff valves 1 In the Local Controller start the maintenance mode and select Change needle seat function OR In the Agilent Lab Advisor software select Service Diagnostics in the system screen Tools Maintenance Positions Change Needle Loop click Start and wait until the needle assembly is in ma...

Page 183: ...le 5 Remove the safety cover WARNING Sharp needle Uncovered needles may cause injuries Do not change the needle seat at this point Do not touch the tip of the needle 6 Turn or flap the wash port to the left 7 In Lab Advisor use Next to move the needle in the down position The needle will move slowly downwards and will stop approximately 2 mm above the seat 8 Disconnect the sample loop from the nee...

Page 184: ...Port Assembly 9 Loosen the fixing screw 10 Lift out the needle 11 Disconnect the wash tubing from the peristaltic pump 12 Release the spring loaded pin 13 Lift the flapper out of the bracket Be careful not to squeeze the wash tubing 14 Reconnect the wash tubing fitting to the new wash port assembly ...

Page 185: ...must be latched into the housing Check the movement of the flapper 16 Install and reconnect the wash tubing to the peristaltic pump 17 Insert the needle 1 Align the needle in the seat then tighten the screw firmly 2 18 Reconnect the sample loop to the needle NOTE Do not overtighten the fitting NOTE Incorrect positioning and installation of the needle loop connection can result in damaging and brea...

Page 186: ...to lift the needle slowly into the up position 20 Turn or flap the wash port to the right 21 Install the safety cover 1 and fix the screw 2 22 Select Next to move the needle into the needle seat 23 Select Back to finish the procedure 24 Close the doors 25 Purge the wash port Check if solvent will be delivered from the peristaltic pump 1 2 ...

Page 187: ...ic Information There is no specific information for this module When The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if a new module with newer firmware is added to a system or if...

Page 188: ...188 Agilent 1260 Infinity II Preparative Autosampler User Manual 9 Maintenance Replace the Module Firmware ...

Page 189: ...0 Parts and Materials for Maintenance Main Assemblies 190 Standard Parts 191 Drawer Assembly 192 External Tray 194 Analytical Head Assembly 900 µL 195 Standard Sampler Accessory Kit 196 Multi Draw Kit 197 Additional Part List 198 This chapter provides information on parts for maintenance ...

Page 190: ...r 66 x 2 mL Vials OR 1 G7129 60110 Drawer for 18 x 6 mL Vials OR 1 G7129 68210 Drawer Kit 100 x 2 mL Vials Classic G7129 60210 Drawer for 50 x 2 mL Vials Classic Left G7129 60220 Drawer for 50 x 2 mL Vials Classic Right 2 5067 4277 2pos 6port Injection Valve prep 600 bar 3 G1313 60010 Gripper assembly 4 G7129 60083 Analytical Head Assembly 900 µL 5 5065 4445 Peristaltic pump with Pharmed tubing 2 ...

Page 191: ...ts Standard Parts p n Description 5065 4445 Peristaltic pump with Pharmed tubing 5063 6506 Finger caps x3 1 1 Reorder gives pack of 15 G7157 60515 Loop for Prep Autosampler G7157 87101 Seat Assy High Flow G7157 87201 Needle High Flow slotted indicated by a white ring 5067 4277 2pos 6port Injection Valve prep 600 bar ...

Page 192: ...66 x 2 0 mL Vials Figure 23 Drawer for 18 x 6 mL Vials Item p n Description G7129 60010 Drawer for 66 x 2 mL Vials G7129 60110 Drawer for 18 x 6 mL Vials G7129 68210 Drawer Kit 100 x 2 mL Vials Classic 1 G7129 60210 Drawer for 50 x 2 mL Vials Classic Left 2 G7129 60220 Drawer for 50 x 2 mL Vials Classic Right G7129 68210 Drawer Kit 100 x 2 mL Vials Classic ...

Page 193: ...r Assembly Figure 24 Drawer Kit 100 x 2 mL Vials Classic 1 Drawer for 50 x 2 mL Vials Classic Left 2 Drawer for 50 x 2 mL Vials Classic Right NOTE It is required that all drawers are put into correct positions that is classical drawer 1 50 must be on the left side and classical drawer 51 100 on the right side ...

Page 194: ...inity II Preparative Autosampler User Manual 10 Parts and Materials for Maintenance External Tray External Tray Figure 25 External tray p n Description G7129 60000 External Tray for 5 x 2 mL Vials G1313 27302 Disposal tube ...

Page 195: ...cal Head Assembly 900 µL Figure 26 Analytical head assembly 900 µL Item p n Description G7129 60083 Analytical Head Assembly 900 µL 1 G7129 27790 Analytical Head 900 µL 2 0905 1294 Metering seal 900 µL 3 5001 3764 Support seal assembly 900 µL 4 G7129 60006 Adapter Assembly Analytical Head 5 0515 0850 Screws 6 G4267 60462 Piston Assembly 900 µL ...

Page 196: ...essory Kit Standard Sampler Accessory Kit p n Description 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste 5181 1519 CAN cable Agilent module to module 1 m G1313 44101 Finger Cap 5500 1155 Tube Connector 90 degree ID 6 4 5500 1254 Tube connector 180 5500 1251 Capillary ST 0 12 mmX 400 mm SL SL 5043 1013 Tubing Clip ...

Page 197: ... extends Injection volumes up to 500 μL or to 1500 μL The Kit contains the following items p n Description G1313 87307 Seat capillary 500 µL 0 5 mm id G1313 87308 Seat capillary 1500 µL 0 9 mm id 5022 6515 Union ZDV NOTE The internal volume of the seat capillary is 400 µL or 1400 µL With the sample loop the maximum draw volume of 500 µL or 1500 µL can be reached ...

Page 198: ...or Maintenance Additional Part List Additional Part List p n Description G7129 68001 Insulation Kit G7129 68000 Air guide kit G7129 60014 Calibration Tool 5023 2540 Adapter ICC Diffusor G7116 67110 MCT temperature verification kit G7129 40050 Transport Protection G7129 20025 Gripper adapter ...

Page 199: ...r User Manual Agilent Technologies 11 Identifying Cables Cable Overview 200 Analog Cables 202 Remote Cables 204 CAN LAN Cables 208 RS 232 Cables 209 USB 210 This chapter provides information on cables used with the Agilent InfinityLab LC Series modules ...

Page 200: ...escription 35900 60750 Agilent 35900A A D converter 01046 60105 Analog cable BNC to general purpose spade lugs p n Description 5188 8029 ERI to general purpose 5188 8044 Remote Cable ERI ERI 5188 8045 Remote Cable APG ERI 5188 8059 ERI Extension Cable 1 2 m 5061 3378 Remote Cable to 35900 A D converter 01046 60201 Agilent module to general purpose 5188 8057 Fraction Collection ERI remote Y cable p...

Page 201: ...e shielded 7 m for point to point connection p n Description RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m p n Description 5188 8050...

Page 202: ...og Cables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 35900 A D converters p n 35900 60750 35900 Pin Agilent module Signal Name 1 Not connected 2 Shield Analog 3 Center Analog ...

Page 203: ...Cables 11 Analog Cables Agilent Module to BNC Connector Agilent Module to General Purpose p n 8120 1840 Pin BNC Pin Agilent module Signal Name Shield Shield Analog Center Center Analog p n 01046 60105 Pin Pin Agilent module Signal Name 1 Not connected 2 Black Analog 3 Red Analog ...

Page 204: ...ension Cable 1 2 m D Sub15 pin male female p n 5188 8029 pin Color code Enhanced Remote Classic Remote Active TTL 1 white IO1 START REQUEST Low 2 brown IO2 STOP Low 3 green IO3 READY High 4 yellow IO4 POWER ON High 5 grey IO5 NOT USED 6 pink IO6 SHUT DOWN Low 7 blue IO7 START Low 8 red IO8 PREPARE Low 9 black 1wire DATA 10 violet DGND 11 grey pink 5V ERI out 12 red blue PGND 13 white green PGND 14...

Page 205: ... 5188 8045 ERI to APG Connector D_Subminiature 15 pin ERI Connector D_Subminiature 9 pin APG p n 5188 8045 Pin ERI Signal Pin APG Active TTL 10 GND 1 1 Start Request 9 Low 2 Stop 8 Low 3 Ready 7 High 5 Power on 6 High 4 Future 5 6 Shut Down 4 Low 7 Start 3 Low 8 Prepare 2 Low Ground Cable Shielding NC ...

Page 206: ...provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Table 12 5188 8057 ERI to APG and RJ45 p n 5188 8057 Pin ERI Signal Pin APG Active TTL Pin RJ45 10 GND 1 5 1 Start Request 9 High 2 Stop 8 High 3 Ready 7 High 4 Fraction Trigger 5 High 4 5 Power on 6 High 6 Shut Down 4 High 7 S...

Page 207: ... 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low p n 01046 60201 Wire Color Pin Agilent module Signal Name Active TTL White 1 Digital ground Brown 2 Prepare run Low Gray 3 Start Low Blue 4 Shut down Low Pink 5 Not connecte...

Page 208: ...o be connected to Agilent modules CAN or LAN connectors CAN Cables LAN Cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection ...

Page 209: ...scription RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m ...

Page 210: ...osampler User Manual 11 Identifying Cables USB USB To connect a USB Flash Drive use a USB OTG cable with Mini B plug and A socket p n Description 5188 8050 USB A M USB Mini B 3 m PC Module 5188 8049 USB A F USB Mini B M OTG Module to Flash Drive ...

Page 211: ...5 Rear View of the Module 216 Serial Number Information 216 Interfaces 217 Overview Interfaces 219 ERI Enhanced Remote Interface 221 USB Universal Serial Bus 224 Setting the 6 bit Configuration Switch 225 Special Settings 227 Instrument Layout 229 Early Maintenance Feedback EMF 230 This chapter describes the detector in more detail on hardware and electronics ...

Page 212: ...properties are the complete communication capabilities CAN LAN USB and RS 232 memory management ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN USB and RS 232 memory management ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into com...

Page 213: ...ct number for example 1315B for the G1315B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 650 is revision 6 50 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Figure 27 Firmware ...

Page 214: ...heir initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original f...

Page 215: ...he module from a computer through a LAN connection This connector is activated and can be configured with the configuration switch With the appropriate software the USB connector may be used to control the module from a computer through a USB connection The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module Th...

Page 216: ...ation The serial number information on the instrument labels provide the following information RQILJXUDWLRQ VZLWFK 3RZHU VRFNHW 1 5 1 86 0LQL 3RUW CCXZZ00000 Format CC Country of manufacturing DE Germany JP Japan CN China X Alphabetic character A Z used by manufacturing ZZ Alpha numeric code 0 9 A Z where each combination unambiguously denotes a module there can be more than one code for the same ...

Page 217: ...d RS 232 Analog APG A ERI E Special Pumps G7104A C 2 No Yes Yes 1 A G7110B 2 Yes Yes No No E G7111A B G5654A 2 Yes Yes No No E G7112B 2 Yes Yes No No E G7120A 2 No Yes Yes 1 A G7161A B 2 Yes Yes No No E Samplers G7129A B C 2 Yes Yes No No E G7167B C G5667A 2 Yes Yes No No E G7157A 2 Yes Yes No No E Detectors G7114A B 2 Yes Yes No 1 E G7115A 2 Yes Yes No 1 E G7117A B C 2 Yes Yes No 1 E G7121A B 2 Y...

Page 218: ...o No No Requires a host module with on board LAN with minimum FW B 06 40 or C 06 40 or with additional G1369C LAN Card G1364E F G5664B 2 Yes Yes No No E THERMOSTAT for G1330B Others G7116A B 2 No No No No No Requires a HOST module via CAN G7122A No No No Yes No A G7170B 2 No No No No No Requires a host module with on board LAN with minimum FW B 06 40 or C 06 40 or with additional G1369C LAN Card T...

Page 219: ...are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card USB The USB interface replaces the RS 232 Serial interface in new FUSION generation modules For details on USB refer to USB Universal Serial Bus on page 224 Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the m...

Page 220: ...ipating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as standard TTL levels 0 V ...

Page 221: ...by start key on any module Receiver is the autosampler 2 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 3 READY H System is ready for next analysis Receiver is any sequence controller 4 POWER ON H All modules connected to system are switched on Receiver is any mo...

Page 222: ...mable input output pins In addition it provides 24 V power and 5 V power and a serial data line to detect and recognize further add ons that could be connected to this interface This way the interface can support various additional devices like sensors triggers in and out and small controllers etc Figure 29 Location of the ERI interface example shows a G7114A B VWD 5 ...

Page 223: ...olled 1 Wire Data Future Use This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI device The firmware can detect the connected type of device automatically and update information in the device if required Pin Enhanced Remote 1 IO 1 START REQUEST 2 IO 2 STOP 3 IO 3 READY 4 IO 4 POWER ON 5 IO 5 NOT USED 6 IO 6 SHUT DOWN 7 IO 7 START 8 IO 8 PREPARE 9 1 wire DATA...

Page 224: ...Use Available by firmware command defined turn on off For devices that need higher power Class 0 0 5 A maximum 12 W Class 1 1 0 A maximum 24 W Class 2 2 0 A maximum 48 W Class depends on hosting module s internal power overhead If a connected device requires more power the firmware detects this overcurrent detection and provides the information to the user interface Fuse used for safety protection...

Page 225: ...ctronics Switch settings provide configuration parameters for LAN and instrument specific initialization procedures All modules with FUSION electronics Default is ALL switches DOWN best settings Default IP address for LAN 192 168 254 11 For specific LAN modes switches 4 5 must be set as required For boot resident cold start modes switches 1 2 or 6 must be UP Figure 30 Location of Configuration swi...

Page 226: ... COM settings are taken from non volatile memory 0 n a 2 2 not assigned Always keep these switches on position 0 off n a LAN Init Mode n a Use Default IP Address3 3 Default IP Address is 192 168 254 11 0 0 0 0 0 Use Stored IP Address 0 0 0 1 0 Use DHCP to request IP Address4 4 Host Name will be the MAC address 0 0 1 0 0 Test 1 System n a n a n a ColdStart Boot Main System Keep Data 0 0 0 0 0 Boot ...

Page 227: ...old start can be used to bring the module into a defined mode with default parameter settings Boot Main System Revert to Default Data The instrument will boot to main mode and changes to the module s default parameter May be also required to load resident firmware into the module Boot Resident System Revert to Default Data The instrument will boot to resident mode and changes to the module s defau...

Page 228: ...uration Switch Table 16 Boot Resident Forced Coldstart SW1 SW2 SW3 SW4 SW5 SW6 Init Mode 1 0 0 0 0 0 Boot Main System Keep Data 1 1 0 0 0 0 Boot Resident System Keep Data 1 0 0 0 0 1 Boot Main System Revert to Default Data 1 1 0 0 0 1 Boot Resident System Revert to Default Data Note The setting 0 down is essential ...

Page 229: ...his pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations...

Page 230: ...n the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need...

Page 231: ...guration Switches 234 Initialization Mode Selection 235 Dynamic Host Configuration Protocol DHCP 237 General Information DHCP 237 Setup DHCP 238 Manual Configuration 240 With Telnet 241 PC and Agilent ChemStation Setup 244 PC Setup for Local Configuration 244 Agilent ChemStation Setup 247 This chapter provides information on connecting the module to the Agilent ChemStation PC ...

Page 232: ... address can be found on a label at the rear of the module underneath the configuration switch see Figure 32 on page 232 Figure 31 MAC Label 2 Connect the instrument s LAN interface see Figure 32 on page 232 to the PC network card using a crossover network cable point to point or a hub or switch using a standard LAN cable Figure 32 Location of LAN interfaces and MAC label NOTE This chapter is gene...

Page 233: ...rk based DHCP Server using the so called Dynamic Host Configuration Protocol This mode requires a LAN onboard Module or a G1369C LAN Interface card see Setup DHCP on page 238 by manually setting the parameters using Telnet by manually setting the parameters using the Local Controller The LAN interface differentiates between several initialization modes The initialization mode short form init mode ...

Page 234: ...figuration Switches The configuration switch can be accessed at the rear of the module Figure 33 Location of Configuration switch example shows a G7114A B VWD The module is shipped with all switches set to OFF as shown above RQILJXUDWLRQ VZLWFK NOTE To perform any LAN configuration SW1 and SW2 must be set to OFF ...

Page 235: ...ion mode Using Stored is selected the parameters are taken from the non volatile memory of the module The TCP IP connection will be established using these parameters The parameters were configured previously by one of the described methods Figure 34 Using Stored Principle Table 17 Initialization Mode Switches SW1 SW2 SW3 SW4 SW5 SW6 Init Mode 0 0 0 0 0 0 Use Default IP Address 0 0 0 0 1 0 Use Sto...

Page 236: ...e He may then close the session select the initialization mode Using Stored power on again and establish the TCP IP connection using the new parameters When the module is wired to the PC directly e g using a cross over cable or a local hub separated from the local area network the user may simply keep the default parameters to establish the TCP IP connection FWLYH 3DUDPHWHU HIDXOW 3DUDPHWHU NOTE U...

Page 237: ...rameters obtained become the active parameters immediately They are not stored to the non volatile memory of the card Besides requesting the network parameters the card also submits its hostname to the DHCP Server The hostname equals the MAC address of the card e g 0030d3177321 It is the DHCP server s responsibility to forward the hostname address information to the Domain Name Server The card doe...

Page 238: ...1 Note the MAC address of the LAN interface provided with G1369C LAN Interface Card or Main Board This MAC address is on a label on the card or at the rear of the main board e g 0030d3177321 On the Local Controller the MAC address can be found under Details in the LAN section Figure 37 LAN Setting on Instant Pilot 2 Set the Configuration Switch to DHCP either on the G1369C LAN Interface Card or th...

Page 239: ...oftware e g OpenLAB CDS ChemStation Edition Lab Advisor Firmware Update Tool and use MAC address as host name e g 0030d3177321 The LC system should become visible in the control software see Note in section General Information DHCP on page 237 Table 20 LC Modules with 8 bit configuration switch B firmware configuration switch at rear of the instrument SW 6 SW 7 SW 8 Initialization Mode ON OFF OFF ...

Page 240: ... non volatile memory of the module It never affects the currently active parameters Therefore manual configuration can be done at any time A power cycle is mandatory to make the stored parameters become the active parameters given that the initialization mode selection switches are allowing it Figure 38 Manual Configuration Principle 7 1 7 6HVVLRQ RQWURO 0RGXOH 6WRUHG 3DUDPHWHU 1RQ 9RODWLOH 5 0 ...

Page 241: ...lect Run Type cmd and press OK 2 Type the following at the system DOS prompt c telnet IP address or c telnet host name Figure 39 Telnet Starting a session where IP address may be the assigned address from a Bootp cycle a configuration session with the Handheld Controller or the default IP address see Configuration Switches on page 234 When the connection was established successfully the module res...

Page 242: ...urn 5 Use the and press Enter to list the current settings Table 21 Telnet Commands Value Description displays syntax and descriptions of commands displays current LAN settings ip x x x x sets new ip address sm x x x x sets new subnet mask gw x x x x sets new default gateway exit exits shell and saves all changes Figure 42 Telnet Current settings in Using Stored mode information about the LAN inte...

Page 243: ...oring parameters Figure 44 Closing the Telnet Session Figure 43 Telnet Change IP settings change of IP setting to Initialization mode is Using Stored active TCP IP settings stored TCP IP settings in non volatile memory connected to PC with controller software e g Agilent ChemStation here not connected NOTE If the Initialization Mode Switch is changed now to Using Stored mode the instrument will ta...

Page 244: ...d Agilent ChemStation Setup PC Setup for Local Configuration This procedure describes the change of the TCP IP settings on your PC to match the module s default parameters in a local configuration see Table 18 on page 236 1 Open the Local Area Connection Properties and select Internet Protocol TCP IP Then click on Properties ...

Page 245: ...nt 1260 Infinity II Preparative Autosampler User Manual 245 LAN Configuration 13 PC and Agilent ChemStation Setup 2 You may enter here the fixed IP address of the module or use the Alternative Configuration ...

Page 246: ...ty II Preparative Autosampler User Manual 13 LAN Configuration PC and Agilent ChemStation Setup 3 We will use the direct LAN access via Cross over LAN cable with the module s IP address 4 Click on OK to save the configuration ...

Page 247: ...2 setup for the 1290 Infinity system using the 1290 Infinity DAD G4212A as the interfacing module The setup works in the same way for all other systems 1 Open the ChemStation Configuration Editor 2 Select from the menu Configure Instruments 3 Select Modular 3D LC System 4 Give the Instrument a name NOTE The LAN must be connected to detector due to high data load on communication to Control Softwar...

Page 248: ...248 Agilent 1260 Infinity II Preparative Autosampler User Manual 13 LAN Configuration PC and Agilent ChemStation Setup 5 Click on OK 6 Select LC System Access Access Point and click on Add ...

Page 249: ...k on OK The Configuration Editor shows now the new instrument 8 If required change under Configure Path the folder locations 9 Save the current configuration via File Save 10 Exit the Configuration Editor 11 Start the Agilent ChemStation During first startup or when the system configuration has changed a notification shows up ...

Page 250: ...t could be configured You may select the module manually from the list We use the Auto Configuration mode Click on Yes 13 Enter the IP address or the Hostname of the module with the LAN access 14 Click on OK The selected module is shown now in the right window with serial number In addition all other modules connected via CAN to the detector are shown as well ...

Page 251: ...the ChemStation loading 16 You may see the details of the module by selecting the module and clicking on Configure Under Connection Settings you may change the IP Hostname of the module may require a re start of the ChemStation After successful load of the ChemStation you should see the module s as active item in the graphical user interface GUI ...

Page 252: ...252 Agilent 1260 Infinity II Preparative Autosampler User Manual 13 LAN Configuration PC and Agilent ChemStation Setup Figure 45 Screen After Successful Load of ChemStation ...

Page 253: ...ent 255 Ground Solvent Lines 256 Do Not Operate in an Explosive Atmosphere 256 Do Not Remove the Instrument Cover 256 Do Not Modify the Instrument 256 In Case of Damage 257 Solvents 258 Symbols 259 Waste Electrical and Electronic Equipment Directive 261 Refrigerant 262 Radio Interference 264 Sound Emission 265 Solvent Information 266 Agilent Technologies on Internet 267 This chapter provides addit...

Page 254: ...s assumes no liability for the customer s failure to comply with these requirements Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards General Do not use this product in any manner not specified by the manufacturer The protective features of this product may be impaired...

Page 255: ...and compliance with safety or EMC regulations Make all connections to the unit before applying power NOTE Note the instrument s external markings described under Symbols on page 259 WARNING Missing electrical ground Electrical shock If your product is provided with a grounding type power plug the instrument chassis and cover must be connected to an electrical ground to minimize shock hazard The gr...

Page 256: ...ification to the product Return the product to an Agilent Sales and Service Office for service and repair to ensure that safety features are maintained WARNING Presence of flammable gases or fumes Explosion hazard Do not operate the instrument in the presence of flammable gases or fumes WARNING Instrument covers removed Electrical shock Do Not Remove the Instrument Cover Only Agilent authorized pe...

Page 257: ...ty Information In Case of Damage WARNING Damage to the module Personal injury for example electrical shock intoxication Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel ...

Page 258: ...elow the boiling point of the solvent used Do not operate the instrument in an explosive atmosphere Do not use solvents of ignition Class IIC according IEC 60079 20 1 for example carbon disulfide Reduce the volume of substances to the minimum required for the analysis Never exceed the maximum permissible volume of solvents 8 L in the solvent cabinet Do not use bottles that exceed the maximum permi...

Page 259: ...oto protocol For specifications of refrigerant charge capacity carbon dioxide equivalent CDE and global warming potential GWP see instrument label Confirms that a manufactured product complies with all applicable European Community directives The European Declaration of Conformity is available at http regulations corporate agilent com DoC search htm Manufacturing date Power symbol indicates On Off...

Page 260: ...y from devices and objects that could be damaged by strong magnetic fields Indicates a pinching or crushing hazard Indicates a piercing or cutting hazard Table 22 Symbols WARNING A WARNING alerts you to situations that could cause physical injury or death Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that...

Page 261: ...ctronic appliances starting with 13 August 2005 NOTE This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation...

Page 262: ... 14 Appendix Refrigerant Refrigerant The refrigerant HFC 134a is used only in the Agilent Infinity II Sample Cooler Table 23 Physical properties of refrigerant HFC 134a Molecular weight 102 Critical temperature 101 1 C Critical pressure 40 6 bar Boiling point 26 5 C ...

Page 263: ... aid instructions After inhalation move the affected person to fresh air keep him warm and allow him to rest If necessary he should be supplied with oxygen If he has stopped breathing or is breathing erratically he should be given artificial respiration In the case of cardiac arrest carry out heart massage Send for a doctor immediately Moreover it must be noted that HFC 134a must always be extract...

Page 264: ...y Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises ...

Page 265: ... s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position 70 dB Sound Pressure Lp 70 dB A At Operator Position Normal Operation According to ISO 7779 1988 EN 27779 1991 Type Test ...

Page 266: ...osphoric acid or phosphate buffer which are less corrosive against stainless steel halogenated solvents or mixtures which form radicals and or acids for example 2CHCl3 O2 2COCl2 2HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol chromatographic grade ethers which can contain peroxides fo...

Page 267: ...ative Autosampler User Manual 267 Appendix 14 Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com ...

Page 268: ... compensation sensor short 135 condensation 28 configuration switche 234 D defect on arrival 59 DHCP general information 237 setup 238 dimensions 29 doors install 153 153 remove 153 153 draw speed 104 104 DRAW 58 104 119 drawer 44 drawers exchange 40 E eject speed 104 104 EJECT 104 electrical connections descriptions of 215 electronic waste 261 EMF early maintenance feedback 230 environment 26 err...

Page 269: ...up 244 TCP IP parameter configuration 233 using default 236 using stored 235 what you have to do first 232 232 leak sensor open 133 leak sensor short 134 leak sensor incompatibility 22 line frequency 29 line voltage 29 local configuration 244 lost CAN partner 131 low volume injections 104 M MAC address 232 main assemblies 190 mainpass 14 maintenance functions change metering seal 113 change piston...

Page 270: ... sample drawers 44 sampler transport 52 sampling sequence 13 sensor leak 22 22 serial number information 216 setup ChemStation 247 shipping 114 shutdown 130 site requirements 26 power cords 27 solvents 266 sound emission 265 special interfaces 221 special settings boot main 227 boot resident 227 specification physical 29 stator 20 step commands 119 step failures 121 step functions 109 T TCP IP par...

Page 271: ...Agilent 1260 Infinity II Preparative Autosampler User Manual 271 Index ...

Page 272: ...cal reference information about the Agilent 1260 Infinity II Preparative Autosampler G7157A The manual describes the following Introduction site requirements and specifications using the module optimizing performance troubleshooting and diagnostics error information maintenance parts and materials hardware information LAN configuration safety and related information ...

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