Page 44
User manual 5448X000 Version 1 June-06
3. Edit keys (
,
,
,
, Ce, Enter, Return)
3.a Cursor keys (
,
): Used to shift the cursor up and down while browsing data and
increase or decrease data while setting time.
3.b Cursor keys (
,
): Used to switch the displayed data while measuring
DNA/Protein and switch the objects while setting time.
3.c Cancel key (Ce): Used to clear off or cancel a previous operation.
3.d Enter key (Enter): Used to confirm and execute the current function, or to finish
parameter setting and quit.
3. e Return key (Return): Used to quit the current function and return to the previous
page.
4. Operation keys (Autozero, GoTo WL , Start/Stop)
4.a Autozero key: Used to set ABS to zero (transmittance 100%). In photometric mode
and quantitative mode, setting ABS to zero(Autozero) is only for current wavelength. In
spectrum mode, it performs baseline calibration in the wavelength 's adjusting range.
4.b Wavelength set key (GoTo WL): Used to set the current wavelength.
4.c Start/stop key (Start/Stop): Used to start or stop reading.
3. TECHNICAL SPECIFICATIONS
Model
4481 Simple beam
4488 Double beam
Photometric system
Simple beam ratio
Double beam ratio
monitoring system
monitoring system
Wavelength range
190 nm-1100 nm
Spectral bandwidth
2 nm
Wavelength display
0.1 nm
Wavelength setting
1nm increment for each scan start and end wavelength's setting
0.1nm increment for GoTo WL setting
Wavelength accuracy
±0.5 nm (with automatic wavelength correction)
Wavelength’s
repeatability
± 0.2 nm
Photometric accuracy
± 0.002 Abs (0-0.5Abs)
± 0.004 Abs (0.5 -1.0 Abs)
± 0.3 %T (0- 100%)
Photometric repeatability
±0.001 Abs (0-0.5 Abs)
±0.002 Abs (0.5- 1.0 Abs)
± 0.15%T (0-100%T)
Stray light
< 0.3 %T (220 nm)
100% T noise
< 0.3% T (at 500nm, 2nm bandwidth, for 2 minutes)
Baseline flatness
± 0.004 Abs (2nm bandwidth , middle scanning ,1100-190nm)
Stability
< 0.002 A/30min (2 hours warming up, 2nm bandwidth, at 500nm)
Version 1 June-06
User manual 5448X000 Page 69
Setting protein factor
Under the self-defining mode, press
(3)
key to display the protein factor setting page.
The system will indicate users to input Protein factor, as shown in picture 46.
At the bottom of this page, enter the
desired protein factor by pressing the
numeric keys
(0~9)
,
(.)
(4.2).
Ce
key (4.3c)
is used to clear off the previous entry.
The system will indicate users to input
the second factor after inputting the first
one. Press
Enter key
(4.3d) to confirm the
numeric entry. If nothing is to be ente-
red, press
Return
key (4.3e) to quit
directly. A 4-digit figure entry is allo-
wed. The range is from -9999 to 9999. If
the figure entered exceeds five digits,
the system will delete what has been
entered. You should start a new entry.
Setting background calibration at 320nm
Under the self-defining mode, press
(4)
key to set background calibration at 320nm.
Two choices are available for this item: YES and NO, which can be selected alternately
each time
(4)
key is pressed.
Setting calibration factor
Under the self-defining mode, press (5) key to display the calibration factor setting
page. The system indicate users to input the calibration factor, as shown in picture 47.
At the bottom of this page, enter the
desired calibration factor by pressing
the numeric keys
(1~9)
,
(.)
(4.2).
Ce
key
(4.3c) is used to clear off the previous
entry. Press
Enter
key (4.3d) to confirm
the numeric entry and quit. If nothing is
to be entered, press
Return
key (4.3e) to
quit directly. A 4-digit figure entry is
allowed. The range is from -9999 to 9999.
If the figure entered exceeds four digits,
the system will delete what has been
entered. You should start a new entry.
Quitting parameter setting of DNA/Protein
Press
Return
key (4.3e) to quit DNA/Protein parameter setting and return to the main
menu of DNA/Protein.
14:57
Input Protein Factor
(-9999 - +9999):
DNA / Protein
Parameter
Means:
1
2
Custom
1. WL (A1, A2): 260.0 230.0
2. Df (Df1, Df2):49.10 3.840
3. Pf (Pf1, Pf2):75.80 183.0
4. 320 nm Adj.: No
5. Adj. Factor: 1.000
Picture 46
14:57
Please Input K
(-9999 - +9999):
DNA / Protein
Parameter
Means:
1
2
Custom
1. WL (A1, A2): 260.0 230.0
2. Df (Df1, Df2):49.10 3.840
3. Pf (Pf1, Pf2):75.80 183.0
4. 320 nm Adj.: No
5. Adj. Factor: 1.000
Picture 47
ENGLISH
ENGLISH