YSI 2900 series, Руководство по эксплуатации и обслуживанию

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8.2.12 Sucrose
This is a direct reading of Sucrose in solution at the enzyme sensor. Three enzymes are co-immobilized in the YSI
Sucrose Membrane: Invertase, Mutarotase, and Glucose Oxidase. Through this chain of reactions the moles of hydrogen
peroxide liberated is directly proportional to the moles of sucrose.
S H
2
O
Invertase
α
-D-G (Fructose)
α
-D-Glucose
Mutarotase
β
-D-Glucose
β
-D-G O
2 Glu Oxidase
H
2
O
2
+ D-Glucono-
δ
-Lactone
System Buffer YSI 2357
Calibrator Std YSI 2780
Linearity Std YSI 2778
Membrane YSI 2703
Membrane
Color Blue
Detection
Range
0.1–25.0 g/L
Calibration
Point 5.0 g/L
Linearity Check
Point 25.0 g/L
Sample Size
25
µ
L
End Point
30 sec
Precision
(CV,n=10)
2% or 0.02 g/L,
whichever is
greater
Linearity
±
2%
(0.1 to Cal Point)
±
5%
(Cal Point to Range
Max)
Typical Working
Life 10 days
Note: See Appendix B – Concentration
Unit Conversion if concentration unit
conversion is required.
Special Considerations:
•
If sample dilution is required, use reagent water or YSI
buffer.
•
The sample must be glucose-free , or at least contain
levels low enough not to interfere with the sucrose
reading. Since the sucrose membrane contains glucose
oxidase, glucose will produce a probe signal.
•
See 8.2.16 Simultaneous Glucose and Sucrose for
sucrose specifications when measuring glucose and
sucrose simultaneously.