YSI 2900 series, Руководство по эксплуатации и обслуживанию

Страница: 108 / 166

107
8.2.6 L-Glutamine
This is a direct reading of glutamine in solution at the enzyme sensor. Two enzymes are co-immobilized in the YSI
Glutamine Membrane: Glutaminase and Glutamate Oxidase. Through this chain of reactions the amount of hydrogen
peroxide liberated is directly proportional to the amount of glutamine.
L-Glutamine
Glutaminase
L-Glu NH
3
L-Glu O
2 Glut Oxidase
H
2
O
2
+
α
-Ketoglu NH
3
System Buffer
YSI 2357
Calibrator Std
YSI 2736
Linearity Std
YSI 2737
Membrane
YSI 2735
Membrane
Color
Magenta
Detection
Range 30–1169 mg/L
Calibration
Point 731 mg/L
Linearity Check
Point 1169 mg/L
Sample Size
20
µ
L
End Point 30 sec
Precision
(CV,n=5) 4% or 15 mg/L,
whichever is
greater
Linearity
±
4%
(30 to Cal Point)
±
5%
(Cal Point to Range
Max)
Typical Working
Life 5 days
Note: See Appendix B – Concentration
Unit Conversion if concentration unit
conversion is required.
Special Considerations:
•
If sample dilution is required, use reagent water.
•
The sample must be glutamate-free , or at least contain
levels low enough not to interfere with the glutamine
reading. Since the glutamine membrane contains
glutamate oxidase, glutamate will produce a probe
signal. See 8.2.18 Simultaneous L-Glutamate and L-
Glutamine to measure glutamate and glutamine
simultaneously.
•
Specs shown are for simultaneous measurement of
glutamine and glutamate.