YOKOGAWA CQ1, Руководство пользователя, Страница: 21 / 268

<1. Function Overview>
1-1
IM 80J01A01-01E
1 Function Overview
1.1 About the CQ1
CQ1 is designed to quantitatively measure biological information from
image data of each cell at high reproducibility. It enables acquisition of
such information as cell functions, signal transduction, cell mobility
(such as invasion) or morphological information from quantified image
data after image processing, which is rather difficult to obtain by
conventional flow cytometetric analysis. Different from flow
cytometetric analysis in which cells are washed away, CQ1 measures
the cells in their culture vessels such as microplate, thus it is possible
to analyze the same cells repeatedly or follow their temporal changes.
Features of CQ1
●
High precision quantification of morphological information without
detaching off the cells
It is possible to precisely quantify biological function or characteristics
of each cell in a natural situation without breaking cell mass or
detaching cell layers from culture dish. In addition to two-dimensional
information, such as the area, various three-dimensional information,
such as volume, surface area, cell number and location, granule
location within each cell, fluorescent intensity, can be well visualized
and displayed as graphs.
●
Live cell observation
Our proprietary confocal scanner unit, CSU series, is a confocal
scanner which can be attached on an optical microscope to enable
confocal observation. The best features of the CSU are the capabilities
of high-speed confocal imaging with a minimal level of cellular
photo-damage and photo-bleaching. Equipped with the CSU, the CQ1
enables three-dimensional and multi-color live cell observation. CQ1 is
suitable for quality control, inspection and experiments of the studies in
cell engineering field, since you don’t have to spoil the cells after
observation.
●
Highly reproducible data
Highly reproducible data can be acquired by stabilizing excitation laser
power with the power monitor function, and also by periodical
calibration to eliminate effects of any other variations.