June 11, 2015, 715004752 Rev. A
Page 102
To rebuild the flow cell:
1. Using the plastic tweezers, remove the new clear plastic gaskets from the Flow
Cell Rebuild Kit, and inspect them for dirt.
2. Drop one clear, plastic gasket into the groove at the bottom of the entrance lens
end of the flow cell body.
3. Inspect the entrance lens, and use nitrogen to blow dust off, if necessary.
4. Using plastic tweezers, drop the entrance lens into the flow cell body,
curved-side facing up.
5. With the tan-colored entrance lens holder curve facing down, use the torque
wrench to tighten the entrance lens screw into the flow cell body to 0.9 N•m
(128 in-oz, or 8 in-lb).
6. Flip the flow cell body over to the exit window side.
7. Using plastic tweezers, inspect the second new gasket for cleanliness.
8. Drop the clear plastic gasket into the groove at the bottom of the cuvette lens
end of the flow cell body.
9. Inspect the exit window for cleanliness. Using nitrogen, clean the exit window, if
necessary.
10. Using plastic tweezers, place the exit window into the flow cell body.
11. With the tan-colored exit window holder facing down, use the torque wrench to
tighten the exit window screw into the flow cell body to 0.9 N•m (128 in-oz, or
8 in-lb).
Tip:
To ensure complete compression of the gaskets, after replacing the exit
window and torquing the exit window screw, and after replacing the cuvette lens
and torquing the cuvette lens screw, you must flip the flow cell body and torque
the entrance lens screw to 0.9 N•m (128 in-oz, or 8 in-lb) a second time.
12. Flip the flow cell body again, and torque the entrance lens screw to 0.9 N•m
(128 in-oz, or 8 in-lb).
13. Flip the flow cell body again to the cuvette lens end, and torque the exit lens
screw again to 0.9 N•m (128 in-oz, or 8 in-lb).
14. With the curved side of the cuvette lens facing up, replace the lens in its screw
mount.
15. With gloved fingers, place the split ring onto the cuvette lens, and press it firmly
until it is flush on all sides.
16. Using the cuvette tool, turn the cuvette lens screw into the exit-lens- mount end
of the flow cell body.
17. Torque the cuvette lens screw to 0.9 N•m (128 in-oz, or 8 in-lb).
18. Follow the procedures in the next section to refit the flow cell in the flow cell
assembly.
Содержание 2489
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